DNA Replication (Prokaryote) INITIATION - Sliding clamp and DNA polymerase loading during INITIATION

[@mcheungtv]

Oh my god, this is TOO good man thank you so much!!!!

[@merttantari7912]

it was so helpful , thank you 🙏🙏

[@beinghimself]

Important question: why are you writing it as if the 3’ end is created first, when we both know there is ssdna looping and the 5’ end is created first because the enzyme is working in the 5’ to 3’ direction

[@beinghimself]

Wait you’re saying 5’3’ but isn’t the helicase supposed to be in the side of the fork where there is a 3’ so it opens in that direction, in that case the upper helicase would be on the right and the lower one on the left. The polymerase of the lagging strand would just move according to the movement of the helicase with a lagging strand that is coiled so that when the fork progresses, the polymerase progresses away when trying to follow the complex, like shown in the famous 3d animation about dna replication, or i’m i misunderstanding the way you’re drawing it kzbin.info/www/bejne/mJbIZ4ONisyDpZosi=SK0PA1cz1429eSDm

[@theCrux]

There are a couple of things to keep in context: 1) The helicase movement looks different for eukaryotes and prokaryotes. 2) The helicases cross eachother at the initiation point (usually going in opposing directions). It may become clear if you sequentially continue with the videos in my playlist on DNA replication.

[@beinghimself]

@@theCrux i suppose that drawings like this are only a simplification of the actual 3d structure, and the strands are already open before the action of the helicases, which is why you didn’t draw them at their corresponding end where they are opening the dna strands, since this is a phase before that?