Mass Spectrometry-Based Proteomics | 2021 EMSL Summer School

Yuqian Gao, a chemist at Pacific Northwest National Laboratory, presents on mass spectrometry (MS)-based proteomics as the method of choice for studying proteins in complex mixtures. This talk covers how proteins can be identified and quantified through various techniques of MS-based proteomics, including discovery proteomics and targeted proteomics, as well as how post-translational modifications can be identified in MS-based proteomics.

[@quynhkhoapham841]

For the discovery proteomics, with a bottom-up approach, the first step after the denaturation is to digest a mixture of proteins in samples to obtain a mixture of peptides. Then, the mixture of peptides is analyzed under LCMS, leading to MS and MS/MS spectrum of peptides. I wonder how we can identify the structure of proteins in the original samples because the MS and MS/MS spectrum obtained with the bottom-up approach are the spectrum of all peptides generated from all the original proteins not the spectrum of all peptides generated from a single protein.

[@chikaugwuodo7899]

This is extremely helpful. Thank you, Dr. Gao!

[@nailaimtiaz3617]

Awesome please make video on illumina sequencing

[@hedayathabibzai6570]

wow it was really helpful presentation Thanks a lot.

[@abdalahmakaranga8335]

Wonderful