Epigenetics3: Histone Modification and ChIP-seq

This module discusses the ways that we study histone modifications in epigenomes, primarily through chromatin immunoprecipitation (ChIP) and ChIP-Seq analysis. At the beginning of this course module you will be introduced to the basics of ChIP and ChIP-Seq analysis followed by a more practical section on how to prepare for ChIP and how to analyze ChIP-Seq results. Finally, you’ll participate in a hands on ChIP-Seq analysis activity and have the opportunity to complete your own research project, if you choose. Learn about it with hands-on examples: edu.t-bio.info...

[@cherkaouihajar5779]

thanks a lot for this video, I have one question, what is the difference between chip seq and MeDIP seq. thx in advance

[@naganaveen10]

Super clear explaination

[@omicslogic]

Glad it helped

[@rameela1000]

Really good one .very clear and constructive

[@omicslogic]

Glad you liked it

[@nissa2468]

Great video! Thank you!

[@omicslogic]

thank you for the feedback!

[@relaxmusic320]

thks

[@saraabudahab92]

Thank a lot for this video! I really appreciate it, where can I find part 1 and 2?

[@omicslogic]

these courses are available on edu.t-bio.info

[@saraabudahab92]

@@omicslogic I think I will take the courses as soon as my semester ends, thanks a lot!

[@raylancamden6858]

you all probably dont give a damn but does anyone know a method to log back into an instagram account..? I somehow forgot my login password. I would appreciate any assistance you can offer me.

[@jamiealvaro8669]

@Raylan Camden instablaster :)

[@raylancamden6858]

@Jamie Alvaro Thanks for your reply. I got to the site thru google and im trying it out atm. Seems to take quite some time so I will get back to you later with my results.

[@mohammedal-hammadi5085]

Thank you so much, it's pretty clear and good video, and your channel is great.

[@omicslogic]

I appreciate that! learn.omicslogic.com

[@reedliu508]

I like your presentation very much! Very clear understanding. Thanks for your hard work.

[@omicslogic]

Thank you! Please visit our website to join these online courses: edu.t-bio.info

[@fizhaadnannn]

can ChIP seq identify all histone modification? because from what i know the efficient technique to identify histone modification is by using sequencing. so can this method also be used to identify all the histone modification?

[@omicslogic]

ChIP seq stands for Chromatin (Ch) Immunoprecipitation (IP) - sequencing (seq). The process starts with specifically designed antibodies that will bind to the histones. After they are bound, these DNA regions are clipped, then the portion of DNA where histones were bound are sequenced. Analyzing the sequences of those portions can help us understand where specific types of histones were attached to the DNA. Learn more: edu.t-bio.info/course/epigenetics-1/

[@MuhammadImran-dv6dr]

great vedio on epigenetic reguations

[@bromaro]

Hello, I remember reading about how epigenetic changes can be prevented from being reversed if they have a lot of chemical markers on them. However, I cannot find the video that talked about this. Could you elaborate on this idea? Thank you

[@NgocTran-tv4lj]

Do histone active or repressive marks present in normal cells, or only in diseases? Thank you

[@omicslogic]

Also cell differentiation is affected by such histone modification. www.nature.com/articles/pr2006122.pdf?origin=ppub

[@briseboy]

For as long as eukaryotic cells have existed, it is likely that CpG methylation, histones, acetylation and deacetylation, and phosphorylation have been selected for, and active in development and differentiation. While any complex system is subject to errors disrupting what we might cell healthy activity, expect that the processes, however complex, arose through selection for optimal utility.