Estimation of Total Protein by Biuret Method || Total Protein Estimation || Biochemistry Practical

Estimation of Total Protein by Biuret Method - This is the video on estimation of total protein by biuret method with demonstration and discussion of viva questions.
It contains
Introduction - 00:49 minutes
Functions of Plasma Proteins - 01:47 minutes
Normal Concentration of Plasma proteins - 03:20 minutes
Principle of Biuret Method - 04:04 minutes
Operational Procedure - 04:40 minutes
Calculation - 05:34 minutes
Normal range of total protein - 06:06 minutes
Causes of Hyperproteinemia - 06:39 minutes
Causes of Hypoproteinemia - 07:29 minutes
Other methods of protein estimation - 07:55 minutes
Discussion of viva questions - 08:23 minutes
Demonstration of Estimation of Total Protein by Biuret Method - 09:43 minutes
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#Biuretmethod #totalprotein #biochemistry

[@timedullantes571]

Hi Dr Amit Thanks for the video. Can I use Biuret method to estimate total protein of any sample from different sources using the same procedure? What is the concentration of normal saline by the way? what standard protein are you using? Thanks for your response.

[@Biochemistrybasics]

Yes you can do but procedure may require some modifications depending on the sources. Concentration of Normal saline is 0.9 % We prepared protein from bovine egg albumin powder Hope it helps.

[@henrikkeegan2760]

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[@kennethjesiah9946]

@Henrik Keegan Instablaster ;)

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[@Chantellynne]

The pump on the reagent is neat!

[@jeremymoore145]

What an awesome video. Thank you

[@Biochemistrybasics]

My pleasure

[@misbagavali]

You explained very nicely 👌👌

[@Biochemistrybasics]

Thanks dear. Please spare a few minutes and do spread the word about the channel among your batchmates and friends

[@beautyblossoms3148]

Sir ethula black colour precipitata agartha close up la katuga

[@musicmaniacARPITA]

Thank you sir..It's so useful

[@Biochemistrybasics]

My pleasure अर्पिता. Please spare a few minutes and do spread the word about the channel among your batchmates and friends

[@shaziasaleh9810]

You have explained very well thanku Soo much sir

[@Biochemistrybasics]

Most welcome dear

[@shubhamkanjilal5545]

Sir, why is concentration of standard 5 gm/dl? Could you explain?

[@bhoomika.v1052]

U saved me from my internals..Ty so much sir

[@Biochemistrybasics]

Wow. Glad to hear that. Please spread the word about the channel among your batchmates and friends

[@pralaymidya9673]

This is so helpful sir🙏......

[@Biochemistrybasics]

Glad pralay you found it useful

[@medicose..5472]

Tq Sir, for such good demonstration

[@Biochemistrybasics]

Most welcome dear. Please spare a few minutes and do spread the word about the channel among your batchmates and friends

[@medicose..5472]

@@Biochemistrybasics yes sir sure

[@Biochemistrybasics]

@@medicose..5472 🙏💫😊

[@soumyapurohit2098]

Great!

[@Biochemistrybasics]

Thanks soumya. Keep loving biochemistry and do spread the word about the channel 😊

[@bipulbera4362]

Hi Sir give a provisional diagnosis of Estimation of total protein by biuret method.. pls sir 🙏

[@Biochemistrybasics]

Ok

[@sciencetricksbyadoctor6171]

You're the God ❤️❤️❤️

[@Biochemistrybasics]

No dear I am just simple human.

[@Amallllll-j8q]

🙃🙃🙃🙃🙃

[@shreyapatel5390]

Well explained sir

[@Biochemistrybasics]

Thanks shreya

[@salihabdulsalam7446]

Thank you sir

[@Biochemistrybasics]

🙏😊

[@champadhali6746]

Thank you so much sir🙏

[@Biochemistrybasics]

Most welcome dear

[@dijidiljith7794]

Sir very useful video. Can you do a video on assay of triglycerides

[@Biochemistrybasics]

Will do. Thanks 😊

[@ceethepoet4909]

Sir why do we have to keep the wavelength the same and also why do we have to incubate the test tubes for 10 minutes?

[@Biochemistrybasics]

It's end point method dts why we have to keep it in incubation.

[@ceethepoet4909]

@@Biochemistrybasics Okay thank you Sir

[@Biochemistrybasics]

@@ceethepoet4909 🙏😊

[@s.shireen9290]

It takes some time for establishment of bonds between Cu N and C ie for chemical reaction

[@Tanimation_]

​@@ceethepoet4909we keep the wv length constant so that we can compare the value with the standard values

[@villagewallah]

Very nice 😊

[@Biochemistrybasics]

Thanks dear

[@ishita6589]

Sir please make on video about imp practicals in 1st year mbbs from biochemistry

[@Biochemistrybasics]

Hey itisha please see practical playlist... All important practical videos are there

[@mahrukhqamar01]

can I do the same biuret method for my plant sample to find the total protein?

[@Biochemistrybasics]

I doubt.

[@sadiajannat6968]

Can you tell me is the reading in decimal? Or its a whole value? Like is the reading of blank is point 06 or just 06? Same as for Standard is it point 26 or just 26?

[@Biochemistrybasics]

It's in point/decimal

[@sadiajannat6968]

@@Biochemistrybasics Thank you so much, Sir for replying so fast!

[@Biochemistrybasics]

@@sadiajannat6968 most welcome dear

[@starpious9997]

Hi sir Is it compulsory to fill the cuvette more than half? Can't we work with less volume ?

[@Biochemistrybasics]

Yes absolutely.

[@foodtechnologist838]

This method wil determine the total protein content.can I use this method instead of kjeldal method.

[@Biochemistrybasics]

Absolutely

[@aishidey2463]

Sir I am really not understanding how to find the concentration of standard...pls explain it sir...I have my exam on monday

[@Biochemistrybasics]

Concentration of standard is pre-defined. It will be given to you. You have to find the concentration of test

[@allroundcomments4578]

👍👍❤️

[@dr.md.muniruzzaman4202]

sir incubation process, in room temperature or in incubator

[@Biochemistrybasics]

In winter you can use an incubator otherwise in tropical countries room temperature will work

[@user-qs3bo3no5c]

Tq sm

[@Biochemistrybasics]

Most welcome 😊

[@navyaarora3662]

Sir .. i was asked in viva that why do we take blank..what is its answer?

[@Biochemistrybasics]

Blank solution contains all of the components of the solution to be analyzed except for the one compound we are testing for. so that the instrument can set zero out these background reading and only report values for the compound of interest

[@jadenjardiolin819]

May I ask what the answer is for the sample values obtained in the simulation at the end of the video

[@Biochemistrybasics]

You can calculate it by putting the value in formula

[@tapankumarnayak5879]

Sir my total protein serum is 8.5...Its little higher and I am panic... please sir suggest me Is is too bad or nt....

[@Biochemistrybasics]

No nothing to worry

[@anonymoususer7869]

It is too bad . Now u have very less chance to survive

[@mileevasankar22]

@@anonymoususer7869 😂😂

[@nitinkumargupta635]

Can we use this method for fluid protein like CSF protein, Peritoneal Protein Urine protein ?

[@Biochemistrybasics]

No

[@nitinkumargupta635]

@@Biochemistrybasics why sir ?

[@nitinkumargupta635]

@@Biochemistrybasics I mean why we can't do with this method ?

[@Biochemistrybasics]

@@nitinkumargupta635 It's done by pyrogallol red method. If using Biuret method then it involves few additional steps that's why not preferred

[@nitinkumargupta635]

@@Biochemistrybasics okay sir n is this turbidity method or another method ?

[@ranjayyadav3477]

Sir what is blank solution

[@Biochemistrybasics]

A blank solution is a solution containing little to no analyte of interest, usually used to calibrate instruments such as a colorimeter and to set reading zero

[@ranjayyadav3477]

Sir why this test is called serum total protein

[@Biochemistrybasics]

Because it measures total protein content which is present in serum

[@Yudu03]

wht is the value of total protein?

[@Biochemistrybasics]

That you can calculate from the reading by feeding into the formula

[@RJ-nj1zh]

Why we called is there any urea🙃 pls say😂

[@EDUCATIONHELPBYSHADABKHAN]

Thank you sir

[@Biochemistrybasics]

My pleasure