Expression vectors: how to choose, or customize, vectors for gene & protein expression

[@sarahnawaz6925]

Hands down the most compact ,precise and well made presentation.🙌

[@weizien5057]

Hands down the BEST cloning lecture I ever seen. Cloning for dummies 101

[@walidel-sharoud8339]

A very interesting, comprehensive presentation of the topic!!

[@goncalofigueiredo5986]

Could you just clarify me in 27:36. When you say n-terminus and c-terminus in the MCS, how could that be possible? Don't they only exist in protein chains? How can the tags be put in n-terminus or c-terminus? Do you put them before translation? But then how do they bind to the resultant protein? So these proteases are a post-translational treatment, how do you insert them into the host? Plasmids?

[@luismatheu4226]

great presentation. ...start with deep expiration

[@AndreasM01]

Nice video-webnimar, clear precise explanations, no more info needed to be added or removed, well done!

[@FootyShorts510]

@Zachary Huxley We dont actually lol

[@wangwang6245]

Thank you so much for such a nice presentation!! very very helpful!!!!

[@LovelyLife365]

Thanks a lot for this very informative presentation

[@nela6188]

You're saving my life

[@mariacristinavillamedina6498]

x2

[@harrycorneliusfleming1399]

@@mariacristinavillamedina6498 x3

[@alejandropikoulasplata5524]

Thank you so much!!

[@biologiaja5795]

Amazing video!!!!

[@paolavillalpandocastro7576]

Amazing video, thanks :)

[@ajaykumardhyani2032]

very useful video...

[@mariacristinavillamedina6498]

Amazing information, thanks a lot!

[@SuperJohnny551]

Amazing Video! Im a neuroscience undergraduate and I am starting to see major effects on how an organism behaves according to certain genetic "markers". The slightly more a protein is made in the cell the greater the effect on the entire organism. Higher concentrations of one protein can have a major effect on the behavior patterns than that of another organism producing less of a concentration. Thank you so much you don't know how much of a help you were. Best wishes!

[@bhavdeep_sharma]

At 3:08 you said cargo might include a functional RNA , in that case transcription alone would be sufficient . But if already contain RNA ,how transcription is sufficient.Please clear the doubt. THANKS

[@randvids6072]

I don't believe RNA is contained in the plasmid. The plasmid is DNA. So if you want a cell to express a microRNA to degrade cellular RNA targets, then you insert the plasmid DNA that is transcribed by the cell into microRNA. That microRNA is not then translated as it is non-protein coding. I think that's the answer you're looking for one year ago!

[@TariqAziz-sg9fl]

would you have ppt on plasmid construction and transfection making the cells and animals cells knock out for specific genes for biological studies

[@MurugeshMurugesh-sk3sj]

How to M13 vector construct using laptop

[@indhumathim3842]

i looking for a vector for my gene . that vector should contain histag in n-terminal , 2 restriction enzymes and there is no extra sequences to be present immediately after the reverse primer .and that vector suitable for ecoli expression system . could you suggest any vector for cloning my gene of interest

[@craig4android]

what is a fusion tag?

[@carlosantoniomontalvonavar743]

Hello !, my project is focused on expression of particular enzymes in a halotolerant bacteria like bacillus okhensis, my main problem is to find an apropiated expression vector (there's no expression vector for B. okhensis, just for B. subtilis). Do you have any datasheet for searching vector for halotolerants?

[@keelayahsleywullumsthegods4493]

DID YOU EVER FIGURE IT OUT?!?!

[@cyrusfirst7531]

Keelay Ahsley Wullums the Godslayer Yeah kind a curious... Did he?

[@rojfarhan9699]

great thank you . how can i contact you please ?

[@sahandkh2458]

thanks

[@markobabic487]

Besides this being the most thorough presentation I've listened to to date this woman has THE sexiest voice ever.

[@FootyShorts510]

Dude i thought i was the only one thinking this LMAO

[@rezakarimi6342]

great

[@kareemrobinson9795]

awesome

[@hamidkiangaikani]

That's a great video but WHY SO FAST?

[@randvids6072]

you can slow playback using the video controls! that helps me - in addition to pausing sometimes.

[@km2052]

nice

[@wisarutsupannafai1599]

Vola!

[@alexklein3357]

translate to spanish please, no any information about this :(

[@jmtholmbo]

I bet you are really cute. You have a pretty voice.