FIJI (ImageJ): Brightness & Contrast Enhancement

[@wangkukson5894]

Thank you for the great video. If the brightness and contrast affect the pixel intensity, do you use them for presentation or analysis as well? I mean, if you have a Group A image and a Group B image, and if you manipulate the brightness and contrast only for the Group A image. In this case, I think the unintentional bias can affect the data. I wonder what case you usually use the brightness & Contrast enhancement. Thank you.

[@johanna.m.dela-cruz]

Brightness and contrast enhancement is a visualization tool. If you had 2 images where you wanted to show a difference in intensity for instance, you can set a certain brightness level that will be used for both images. This would provide qualitative information.

[@wangkukson5894]

Thank you for your kind reply. You mean the brightness and contrast are used for a qualitative goal, like to show good image contrast for a publication or an article, after you are done with all of the quantitative analysis, right?@@johanna.m.dela-cruz

[@johanna.m.dela-cruz]

@@wangkukson5894 you can also use contrast enhancements for easier segmentation.

[@wangkukson5894]

Thanks a lot. Didn't you set up anything for donation here? you deserve it. @@johanna.m.dela-cruz

[@nichtjes4ever]

Thank you for the video! I have a question: Does changing the gamma factor alter pixel size?

[@johanna.m.dela-cruz]

Hi Laura. Thanks for watching. Changing gamma is a nonlinear adjustment of brightness/contrast. It does not change pixel size. It does, however, change intensity values.

[@nichtjes4ever]

@@johanna.m.dela-cruz thank you!

[@roberto31589]

very good, thanks!

[@johanna.m.dela-cruz]

@roberto31589 Thanks for your support.

[@asmaelaouina7047]

Thank you very much! it was very helpful

[@johanna.m.dela-cruz]

Hi @asmae laouina. I’m glad to be of service. Thanks for watching.