Mam, How is the complementary strand to the target sequence removed before hybridization? Because it may happen that during hybridization or cooling, the target strand again bind to the complementary sequence instead of probe
@sakshamsiddhu47373 жыл бұрын
Ma'am why point mutations can't be detected by fish?.....and why pcr can't be used for large gene mutations?
@muktisharma69783 жыл бұрын
One single point mutation SNP on DNA cannot be hybridized by labeled probe, because we are using the whole intact chromosomes..and still SNP DNA can also hybridized and denatured it.... In case of multiple mutations, PCR will detect easily with use of multiple probes...allele specific probes...
@vidhigualian90133 жыл бұрын
Thnk u mam great explanation 🙏
@dr.poonamagrawal61963 жыл бұрын
Most welcome 😊
@simhadriMD4 жыл бұрын
I am very thankful to you Ma'am.
@dr.poonamagrawal61964 жыл бұрын
So nice of you
@nakulsampat16024 жыл бұрын
Thanks a lot for excellent explanation
@dr.poonamagrawal61964 жыл бұрын
Glad you liked it
@tukombemuleya96893 жыл бұрын
Thank You Ma'am for your great explanation. this is Great
@dr.poonamagrawal6196 Жыл бұрын
You are most welcome
@sheraziz1473 жыл бұрын
Thank you Mam but I need some academic information from you plz