From Ender 3 to DIY Syringe Pumps (Microfluidics capable)

[@v_saggiomo]

all the files and instructions are here: github.com/Vsaggiomo/Ender3-syringe-pumps/blob/main/README.md

[@ehtashamrahat1473]

That's wonderful Vittorio. This is Rahat from MKK lab. This detailed instructions will be very helpful during the experiment

[@SJMNin]

That's amazing. Thanks a lot for the detailed instructions!. It is so kind of you that you share that experience! Congratulations!

[@v_saggiomo]

thanks :) let me know if you build a set

[@Engineering_Science]

Very creative got your idea working using off the shelf parts.

[@raniersepulveda4626]

We are building one right now. Thanks for sharing.

[@zimboiii9025]

Awesome stuff my man!

[@markgrayson4460]

Curious can this be used to fill the syringes? Possibly with a thicker fluid or even grease or paste?

[@v_saggiomo]

Withdraw yes, that’s possible. Paste and thick liquids I guess not as the motors are not that powerful

[@yamantayyar]

Thank you for the great work. Wondering if you can use different syringe volumes in different channels at the same time? Do you have an example on this? Cheers!

[@v_saggiomo]

Yes, it is possible to use different syringes on different pumps, you just need to change the M92 commands, and yes it is possible to use different flow rates for each channel. From our paper: ________ Multiple syringes can be used at the same time: G1 X0.5 Y0.5 Z0.5 F1 Will deliver, at the same time, 0.5 mL of liquid from the pumps on X, Y, and Z at a speed of 1 mL/min For using different flow speed for different pumps, the total amount of liquid should be calculated taking in account that all the pumps will reach the end at the same time. For example: G1 X2 Y1 Z2 F1 Will deliver the set amount of liquid at different flow speed, using 1 mL/min on Y, and 2 mL/min on X and Z. __________

[@aishwaryasinha579]

Hello from India. Firstly, a great video... and such amazing creativity... wow! I'm a doctor, and I'm also associated with an NGO here. Was planning on donating an infusion pump for a long time now... but it was too costly. If anyone is interested in selling one of these to me, I'd love to connect with them to discuss further details. Thanks. 👍🏻😊

[@ashwinkumarnarasimhan4604]

Can you tell me how could you manage to view those droplets using cell phone.? Looking for such a unique model for my microfluidics work. You knocked it. Thankyou so much.

[@v_saggiomo]

this is quite a "big" microfluidic device (1mm) so the flow is quite slow and ca be recorded at 30fps without any problem

[@ashwinkumarnarasimhan4604]

Hey.. thankyou so much for the information. 1mm is the channel dimensions.? Also how do you program to set for 10uL/min.? I mean the G-Code.

[@v_saggiomo]

@@ashwinkumarnarasimhan4604 yes, 1mm channel dimension. The gcode for 10uL/min would be something like G1 X1 F0.010 F is the speed in mL/min. but better read the GitHub and the preprint for a better explanation: github.com/Vsaggiomo/Ender3-syringe-pumps

[@phoema80]

Ciao Vittorio, credi che il tuo approccio possa essere usato invece per fare una stampante con siringa? usare i controlly della stampante per un motore NEMA17 o bisogna riprogrammare la stampante?

[@v_saggiomo]

sicuramente, ci sono gia' vari modelli in giro. Il piu' semplice è quello di usare l'extruder motor come syringe pump: www.biorxiv.org/content/10.1101/2021.03.10.434735v3.full.pdf ma ci sono anche modelli piu' complessi dove la siringa sta direttamente sopra il printhead: www.hardware-x.com/article/S2468-0672(17)30082-2/fulltext www.hardware-x.com/article/S2468-0672(18)30087-7/fulltext cerca "bioprinters"

[@phoema80]

@@v_saggiomo sei un grande grazie

[@karelchramosta1378]

Hey, recently I tried to build the pumps using the dual+50ml adapters, but the holes for the rod are not aligned, is there any fix exept making holes in the 3d printed parts?

[@v_saggiomo]

it'a a standard Ender 3? we (and other groups have rebuild it without any problem), are you sure you are using the right axis? can you drop me an email with some pictures?

[@karelchramosta1378]

@@v_saggiomo there is no problem with bought parts, I sent you an email with photos of the problem.

[@rover1102]

Would this work on an ender 5?

[@v_saggiomo]

well, yes in general terms (programming, motor steps and so on), not sure if the 3D printed adapted parts are the same

[@fikretkus106]

I really don't understand. Did you really waste the whole 3D printer to make a syringe pump?

[@v_saggiomo]

yup, a single syringe pump costs 400€, this 3d printer costs 150€ and you can make 4 syringe pumps...

[@fikretkus106]

@@v_saggiomo Thank you for your kind reply. I've got your point but still you don't need to waste the printer. All you need a NEMA 17 step motor, 2 steel rod, 2 lmek 08 uu linear bearing, 2 8mm bearing, a motor coupler, a lead screw with its nut and the arduino. The other parts could be printed. All of them takes 28.27 € acoording to prices in Turkey. I tried it and it works fine. By the way 400 € looks like a good price :) I can deliver hundreds of them for 200€ with a high quality metal parts and touch screen :)

[@v_saggiomo]

yes, of course you can, but that's not the point. The point is that in a single box you have everything for making them