GAMSAT Biology Crash Course #2 | Cell Structure, Cell Cycle & DNA | Section 3 For NSBs

[@wafaaadam5611]

Hello Jesse, I think that for q4 it would only be true if you were to count Interphase as part of mitosis, however since it is only part of the cell cycle and not mitotic division the GFP and BFP should remain the same throughout mitosis as the DNA is replicated in the synthesis stage of the cell cycle. Thanks so much for the great content, keep it up!

[@jesseosbourne]

Hey Wafaa! That's a good call Given the question states during mitosis, the 'amount' of DNA would not be varying as replication has already occurred before mitosis began so it should be constant given we are talking about total abundance in the sample. As a note, if it stated that abundance was measured as an average per cell, then the answer would be that it varies as the DNA per cell would 'halve' in that replicated DNA would be divided into the two daughter nuclei during late mitosis. You know what's weird? When I was in first year of my undergrad (2011) we were taught that replication occurs during prophase and not interphase. It was my first time learning bio and it made quite the impression to the point I still have to google it to double check which it is haha. No idea whether the scientific consensus was different then and I'm old, I was taught wrong, or I just wasn't paying attention in those lectures and got it mixed up myself 😂 I think in the video, I'm actually slipping back into old habits of assuming replication in prophase

[@wafaaadam5611]

@Jesse, thanks for letting me know. Tbh I also had to double check to ensure I understood. Knowledge is so fluid that new research can either support or disprove previous ones. Thanks for all your hard work!! I highly appreciate it and I'm sure many do too!

[@tnn1791]

thank you so much Jesse, wish I found your videos earlier, I used MCAT textbooks to study for GAMSAT and on the actual test date I was overwhelmed by the information from questions and didn't have enough time to answer. Your videos are very helpful!

[@Esther13young]

Jesse, I am so thankful I found your channel. You are an excellent teacher and I love how you teach not only content but also how to think about a question. Thank you SO SO much! (Med school would be better with you in it)

[@jesseosbourne]

This is so great to hear, Esther! Really glad that you've found these helpful :) Best of luck with the study!

[@gaymer_aus2371]

Thanks so much for your videos, they're an amazing resource. Your website is amazing, I kept waiting for a paywall and nothing. Thanks so much! Going for my first sitting in March 2022 and I've left the prep till this month. Thanks so much!

[@jesseosbourne]

Thanks man! Hopefully these videos and the resources page will help speed up the study process for you :)

[@sofiajimenezsanchez]

We don't deserve you Jesse!!!! Thank you for yet another great video, you absolute legend!

[@jesseosbourne]

Thank you, best of luck Sofia!

[@blad3wing2]

You are now my favourite youtuber! Excellent material and clear explanations.

[@jesseosbourne]

Really appreciate this, and glad it's been helping!

[@crazztexxart998]

46:24 A forms 2 hydrogen bonds with T C forms 3 hydrogen bonds with G

[@Chatswithlulu]

You are AMAZING! I am so thankful for your incredible content - I love your work and appreciate it immensely!!!

[@arminadouzandeh5859]

your actually so good! thank you for helping us out

[@jesseosbourne]

Not a problem. Thanks for watching, Armina!

[@kamaldhillon8223]

I think this community loves you so much. “Stay blessed”.

[@jesseosbourne]

Thanks Kamal! I appreciate this and the very supportive and positive comments section we have on this channel 💙

[@NabellaEarth-Reda]

your videos are so great! been a bit lost with what content to study and what to focus on, the Des O'Neil books really confused me. Your videos have definitely saved me lately so cheers

[@jesseosbourne]

Yeah Des can be pretty overwhelming at times as they can sometimes be dependent on knowledge of outside info and formulas. Glad this stuff has helped!

[@manirashti5433]

Amazing Jesse, Thanks for the effort to make the life easier like me. Cheers 👍

[@moonlighting9785]

Thank you so much. It was a very informative video

[@didisaythankyou]

well done

[@user-yr5tx7wr9x]

Amazing videos! I discovered you a couple months ago when I started looking into affordable tutoring. Your videos seemed too good to be true! I'm sitting the GAMSAT for the second time in March and I hope with your videos I can finally get into med! When do you think you'll release your intermediate level videos? I'd find them super helpful!

[@jesseosbourne]

So great to hear that these videos have been able to make such an impact I your study (and probably save you some cash in the process! haha) Yep, I have a chemistry/redox set of questions coming this week and will aim to do one set of questions a week for the next few weeks to build up some intermediate level stuff ahead of March!

[@brendanhon5891]

Thanks again Jesse

[@surekanrajendran652]

I always get caught out with understanding the wording and it's so frustrating! Like in question 2 when it mentions 'for a DNA sample binding the GFP carrying probe', I assumed this meant the wavelength measured on the graph would double. Maybe it was seeing the word binding that sent me off on a tangent but it made me lean toward the last answer option thinking the peak would be at roughly 11.

[@jesseosbourne]

It’s definitely frustrating when you realise you’ve misinterpreted the question. It’s half the battle of gamsat, I feel haha You’ll always learn something from each mistake though.

[@evolvewithme3314]

Hi Jesse, for Q3 why is C the correct option when B also had the peak near 500nm? Love the videos by the way, thank you for the detailed explanations!

[@kdaray8010]

Amazing bro!!!

[@jesseosbourne]

Thanks man, glad you liked it!

[@serenek3083]

Hello, if 3' AGTCC 5' is the "target sequence" of the probe, does that not mean that the probe binds to this sequence? therefore shouldnt the answer to question 1 be B?

[@jesseosbourne]

Hmm that's a good call, I probably shouldn't have used the phrase "target sequence" as I believe I meant that this sequence was part of the probe itself. I'll adjust the wording of the question but with that interpretation yes you'd be right!

[@serenek3083]

@@jesseosbourne thank you for your response!

[@furbytvtv]

Nice shirt! He scrubs up well 🕺🏼

[@jesseosbourne]

colour gang! 🎨

[@iarfiel]

Hi Jesse. I have been working through your videos and questions (thank you so much by the way, these have been a huge help in refreshing my memory and re-learning a lot of topics in English) in preparation for GAMSAT September sitting (almost there!). In regards to Q4. I listened to your explanation and saw the pinned comment. When I first saw the question, my answer was C - Abundance for both GFP and BFP would not change, and my reasoning was that even though DNA amount in the cell could be varying throughout the mitotic division, the amount of probes will not, hence as they had already binded to the maximum amount possible when introduced, they could not express more GFP/BFP when more DNA is intrododuced as they have reached their max expression potential. Have I completely overthought this or was my thinking incorrect? Thanks!

[@jesseosbourne]

Hey Sandrina, another really good point! Your reasoning is also right, particularly because int he actual stem I wrote that abundance is defined as the hybridisation of probe to DNA so without more of the probe, doubling the DNA would have no effect on the amount of DNA-probe binding given he amount of probe is still unchanged so this is an alternative explanation to why C is the answer! Great thinking Hopefully my later questions were a little less messy than this one 😅 Good luck for September!

[@user-vy8vm5kj7t]

Hi Jesse great video thank you! Just one question, for Q3, why does the BFP complementary DNA base pair sequence stay from left to right, but for GFP, you reversed it to be from right to left? I understand that technically this is because the carbon numbers were reversed but I still don't get how to understand this fully to differentiate in future. Thanks! :)

[@jesseosbourne]

Hi Paula, DNA when bound together into a double stranded molecule run 'antiparallel' meaning they run in opposite directions to one another in terms of their 3' (free sugar) and 5' (free phosphate) ends. Rather than using left to right and right to left, you want to describe direction as 3' to 5' or 5' to 3' as the molecule can simply be rotated around and suddenly left and right ends gets very confusing. You'll notice that in the answers in Q1 they are written 5' to 3' but in Q3 the big sequence given is flipped around 3' to 5' . The GFP sequence is the same as in Q1 however it appears reversed because the whole molecule is flipped around in Q3 (that's one of the tricks to look out for in this stem). So in general always be on the lookout for the 3' and 5' ends to orient yourself as this is a common trap in DNA questions. Hope this helps!

[@menghowlee332]

Hey Jesse! Loving the crash courses! Just wanted to ask if it would be beneficial to learn the connections/network of the endomembrane system, and details of it - I.e. receptor mediated Endocytosis, translocation and SRP’s, etc. Or would this already be taught in the stem of questions?

[@jesseosbourne]

Nope, that'd be too specific. If it's not in the crash course then it'd be outside of what I think is relevant knowledge

[@menghowlee332]

@@jesseosbourne noted! Thank you for the guidance. Truly appreciate it.

[@StephanieSalvatore-r5i]

Chicken scratches .. HAHAHA! That't too good, hahah

[@joycehoughton379]

Any advice for September gamsat sitters? 2022

[@jesseosbourne]

I'd say the slow and steady approach is the best method with 6 months of preparation time. A little bit of study most days over a long period of time is much more sustainable and effective than 12 hours a day for a month. In terms of study tasks: Diagnostic test (ACER Practice Questions) --> Content Review --> Practice Questions & Reflection --> Timed Practice --> Full Exam Simulation For Section 2: Write essays --> Get feedback --> Research unfamiliar topics and concepts --> Read essays --> Write essays --> Get feedback and repeat

[@megaadidas98]

@@jesseosbourne Hey i've been going through your content and love all the information you've been giving, thank you so much, Quick question what advice/study method would you give for 3 month crash course with someone non science background, i have an undergrad/masters in law and feel comfortable with s1,s2 but with no science background what would you advice, thank you again

[@jesseosbourne]

Hey @Mozart W, pretty much the same advice as I've given above. being NSB you might run into more issues with getting to grips with the speed at which you process the stems because of some jargon but the depth of the theory is very shallow in most cases. If you find you're struggling to understand what it's telling you, then it might be worth reviewing that section of the Crash Courses or use Khan Academy videos to strengthen your understanding before jumping back into the questions. Best thing to do is use the practice questions to learn about 'flexible' skills that could be developed and used not only in that stem but in others as well rather than just aiming to 'get the question right'. Best of luck!

[@megaadidas98]

@@jesseosbourne Thank you so much for the advice, I've been going through your crash course, Looking forward to more videos! Is it possible you could go through the structure of how to go through a question within the time limit, tactical approach please. Thank you again

[@aditiganguly9794]

👍