Gene knock Out | Neomycin | G418- Casette I Experimental | PYQ included | CSIR-NET JRF |GATE | DBT

[@BaaYo]

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[@BaaYo]

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[@sakeenabatool1002]

Literally ma'am, I was just searching on this topic. And I got your notification

[@BaaYo]

Glad it was helpful!

[@aadilbhat4309]

Very Nicely Explained Thank you ❤

[@Maitri24]

You are excellent superb fabulous fantabulous awesome Teacher a big big big thank You dear mam

[@bhavikapawar1999]

Thank you mam for explaining this concept 👍🏻

[@mayurransing5205]

2 minutes silence for those students who don't find this type of fabulous Explaination😁😁

[@deepakNikash2]

Right

[@crazycoins8923]

Thank u mam Your way of teaching superb And you make tough topics easy magically

[@Zama528]

Home work pura hua mam 😀 Thank you ❤️🙏

[@firdousahmad6000]

Wonderful explanation mam... Answers of the given sequence of questions will be 2,3 and 4 respectively

[@BaaYo]

🙂

[@maheshswami5226]

It was very nice video mam , hamesha ki tarah👌👌👌🙏🙏

[@BaaYo]

Thank you Mahesh

[@kapilgrover7767]

Best teaching

[@Reettu153]

Thank You ma'am for these series. I literally afraid of the unit 13. Finally it reduces some.

[@BaaYo]

You can do it!

[@Raj-mr9vg]

Bro you are good doctor lol

[@KShiv2024]

Very nicely explained. Thanks ma'am

[@BaaYo]

Thanks you

[@Studentkaksh]

Wow mam Best explanation 👌🏻👌🏻👌🏻❤❤

[@ankitasamal8067]

Loved your video maam❣️I was searching for this video.

[@nasreenshaik19]

Answer will be B ,C ,D respectively , thank you ma'am for this awesome lecture and saving me before exams 🥲

[@sandeepkalita7125]

Ma'am, keep up your great work. Best wishes.

[@manikbrahemi7468]

Best explanation

[@abhishekrana5933]

Transgenesis or Geneknock out mai kya distiction h mam ?

[@mahnoormehar12]

Assalam o alikum! Mam plz crisper Cas9 par b Aik vedio bnaye plz

[@amitsahoo82]

Mam please explain resistance and sensitivity

[@chinmayeemohanty9488]

6-b,140-c,135-d

[@atulsaini5493]

Thanku mam for this class

[@tushargupta8301]

Thank you so much Ma'am for the informative video. I had a question regarding transfer of gene as said after electroportion vector gets to the genome and the genes get switch, so how the tk+ gene went in the ectopic and not in ideal cell...

[@meeramishra8213]

Mam G148resistance ke liye kya responsible he????

[@harini8193]

Ma'am.. I've a doubt.. in ectopic insertion why the "tk" gene got inserted along with the gene of interest ..

[@deepakNikash2]

Well done mam

[@hi-bg6uv]

6-D, 135-C, 140-B. Thank you mam😍

[@BaaYo]

try again.. their is some mistake

[@renukamaurya1966]

Thank you ma'am ❣️

[@hi-bg6uv]

6-B ---bcoz cell survive in the presence of G14 & ganciclovir 140-C :- no transition 135 - D :- not crrct bcoz it is non homologous define Thank you for crrecting me mam😍 again video dekha acche se thrn qusn solve kiye..phle jldbaaji krdi thi😋

[@BaaYo]

Perfect ❤️👏👏👏👏👏

[@hi-bg6uv]

@@BaaYo 😄

[@chemistmuhammadbaqir7249]

Please one vedio on monthly calibration of FTIR Nicole iS5 thermo performance

[@GhulamHussain-bw9tj]

Pls specify its application. Which product u r analysing in ftir?

[@BaaYo]

noted

[@nesabfiroz1249]

Mam 6. Answer b kaise ho rha please explain

[@senjutinath3931]

6.b 104.3 135.2 and 4

[@VarshaThakur-x7t]

What's the use of tk gene in vector mam

[@BaaYo]

It is helping in selection of negative cells. Jin cells me random integration hai vo tk gene express krti hai

[@VarshaThakur-x7t]

Thank you mam

[@rituparnakarmakar6723]

Nice nice nice, thank you so much🥰

[@BaaYo]

😍

[@harshvinayak2428]

Question1 :- B Question2 :- c Question3 :- D

[@vaishnavibhardwaj9970]

Mam I do not understand the last few steps having some confusion Please clear I'm preparing for an exam Confusion is that you said at 20.13 that one gene is delete and 1 is functional i understand about the deleted gene but which gene is functional here I mean we take only 1 gene here than which one is functional getting confused here please clear

[@ranishaw7545]

6- B, 140- C, 135- D. Mam check the answers

[@BaaYo]

Correct 👏👏

[@akhilthota3860]

@@BaaYo i dint understand why 140 - 3 is the correct answer. Can you please explain mam

[@rononoazoro1808]

@@akhilthota3860 he made a construct that was sensitive to neomycin ( so the neomycin gene will be outside of the homologous terminal region and will be left outside even the recombination is taking place ) and resistant to ganciclovir ( meaning the TK gene will also be outside) hence answer is 3 as both the gene will be outside of the homologous terminal regions

[@manharkrishnaojha5252]

Ma'am gene targeting topic pr video bna dijye

[@doraemon6853]

Ques: 6_D, 140_C, correct or not? 135 nhi samajh aarha ma'am help me

[@chemistmuhammadbaqir7249]

A. O. A Dear respected Mam

[@khushbubedi1426]

Thanku

[@BaaYo]

🤩

[@susmitabose7230]

2 and 4 are correct answers

[@de2jx]

Thank you maam.

[@jyotibagda3990]

6-b 134-3 135-4

[@rudra7847]

Maam 140 no question 3 ans kaise hoga

[@triptisingh8137]

Thank you ma'am 🙏

[@BaaYo]

Tripti Please share with your batchmates also

[@triptisingh8137]

@@BaaYo sure mam

[@pinkiyadav853]

Ques1 (2) Ques2 (3) Ques3 (4)

[@BaaYo]

well done

[@naveenshankar4493]

Add english subtitles guys...

[@rachanasinha2113]

Ma'am can't understand the last question,why the answer is option D? Please help

[@BaaYo]

They have asked for incorrect statement. did you notice that?

[@rachanasinha2113]

@@BaaYo Yes ma'am. I am not able to understand the neo gene insertion site.

[@nehaphogat6648]

1:-B 2:- c 3:-d

[@poojagujjer118]

Thanks🌹🌹 mam

[@BaaYo]

❤️

[@maheshswami5226]

Mam, tk ko outside kyo rakha hua h?

[@BaaYo]

correct and ectopic insertion ko identify krne me help kreta hai . Agar vo cassette ke beech me hoga to vo bhi neo ke sath switch krega aur cell ke genome me integrate ho jayega aur express hoga. to cell ganciclovir sensitive ho jayegi. Hope its clear

[@maheshswami5226]

@@BaaYo thank you pyari mam🥰🥰🙏🙏

[@dhartibandarwar1917]

Ans B C D

[@a2z440]

🙏🙏💕💕

[@diyashideb2731]

6B 140C 135-4. These are the answers maam. Are they correct? Thank you so much maam for ur efforts🙏❤️

[@BaaYo]

[@harishbhatia3754]

Hlo didi aapse kuch guide leni thi

[@BaaYo]

btaiye

[@harishbhatia3754]

@@BaaYo muje bhul gye kya didi

[@akankshasinha5063]

140 ) 3

[@akankshasinha5063]

6. 2

[@akankshasinha5063]

135. 4

[@rajakkhan3701]

can we contact you