thank you so much for your presentation. God bless you
@George-rq1yp Жыл бұрын
Excellent! You know how to explain stuff!
@hengzhao3082 жыл бұрын
Th!is is a wonderful series of teaching about gene expression analysis! Thank you so much, Mike!
@muratseker64063 жыл бұрын
thank you for the videos they are quite helpful
@muratseker64063 жыл бұрын
for some reason I come across your channel by chance. KZbin algoritms were not helpful
@jems68403 жыл бұрын
Thank you for this wonderful tutorial. I do have a question though : When I run GO analysis for my upregulated DEGs ( p < 0.05), 99% of the GO terms associated with specific genes have padjusted value greater than 0.05 and a lot of them have pvalue of 1 , Why does this happen? If I am interested in a Gene X in a treatment group that is differentially upregulated but the GO terms have p-value > 0.05 how do I interpret it ?
@mikevandewege30073 жыл бұрын
So your genes may be upregulated, but there may not be any specific functions or pathways that are biased among upregulated genes. The adjusted p-value corrects for multiple tests. A p-value of 0.05 still means 5% of tests will be significant by chance, sooo we try to filter out the most likely false positives. Hope that helps.
@khanmohdsarim82112 жыл бұрын
After StringTie I have locus tag and read count, which I have used for Deseq2, Kindly inform me how to convert these Locus tag to gene id because Panther database is not recognizing Locus tag ID and giving all unmapped result. Thanks
@PhanCanhTrinhPlus2 жыл бұрын
Hi Mike, I am trying to use Panther for Klebsiella pneumoniae with my protein list. Is it possible to use for K. pneumoniae. I tried many time but I cannot get any result. Please help me if you know. Thank you very much! (example ID: M7PZH9 M7QKV7 M7PNJ0 M7QE19 M7PB39 M7PY01 M7P355 M7QCU5 )
@alessiaartusi523311 ай бұрын
Hi, is there some package or function to plot the results?
@mikevandewege300711 ай бұрын
For those results specifically, not really. However, I have used an R package (clusterProfiler) that helps make nice enrichment plots.
@ds5375 Жыл бұрын
To save you 34 minutes. This video does NOT show you how to download and parse the files.
@nitinshukla9993 жыл бұрын
mike I need more tutorials videos .. what next from here. please update
@mikevandewege30073 жыл бұрын
Recognize this was all just for my bioinformatics class during covid that i'm leaving up. I really have no intention of making new videos. Were you looking for anything specific?
@lidiaryabova2 жыл бұрын
@@mikevandewege3007 Dear Mike, I found your explanation really really good .If you pan to do something , please make video on gene annotation with different methods please ..
@saravalero66792 жыл бұрын
why if I introduce 20 genes, there are 20000 results??
@vishweshbharadiya67582 жыл бұрын
Oh mann no statistically significant results uhhhh 😫😫