Homologous Recombination for Double Strand Breaks Part 4

In this video we discuss the use of homologous recombination in the repair of double strand breaks.

[@purpearl1]

I DONT KNOW HOW TO THANK YOU , I WAS SEARCHING FOR CLEAR EXPLANATION OF THIS MACHANISM BUT I FINALLY EVERY HINGS ARE CLEAR TO ME THANK YOU VERY VERY MUCH

[@anasaldarriaga9362]

Thank you very much! You are a great teacher, you help me more than any other. This is my thesis topic and after reading a lot of papers you were the only one who lightered the path of understanding

[@Satkat_Ind3981]

You are a fantastic teacher. The most diffiult thing innsciencek is to make it simple and you have mastered it. Thanks

[@sheMessages]

I had to pause it just to say this vid is amazing!, thank you!!

[@anerolvial]

Loved your video. Found it very useful, since I am now going over CRISPR/Cas. Thank you!

[@crocketmeow]

I like the comprehensive explanations; questions: In prokaryotes, strand invasion is mediated by RecA. The eukaryotic analogue is Rad 51. In prokaryotes, branch migration is mediated by RuvAB. What are the eukaryotic analogues of RuvA/B? In prokaryotes, Holliday junction resolution is mediated by RuvC. What is the eukaryotic analogue of RuvC? In prokaryotes,RuvD cuts the strands horizontally or vertically. What is the eukaryotic analogue of RuvD?

[@annaiahramesh1542]

I am really very much thankful to you for your excellent explanation. But still I don't know how all of a sudden H2A histone protein replaced by H2AX. Because DNA damaging event is random and H2A is canonical histone protein. Another one is recombination part, but it's hard to accept the mechanism how it happens between Homologous chromosomes. Any how right now this is accepted model by the scientific community, let we look forward for more refined version. Once again thank you very much.

[@badmadmat20]

this is delightful! very detailed. love it

[@lilianavertel9789]

You are the best!

[@annaiahramesh]

informative and very nice explanation. Thanks a lot

[@indocampus2002]

thank you very much for your explanation..it really helps me!

[@junxing9680]

Thank you very much, very specific explanation

[@pragha1]

Great video! I have just one question. How does it know when to stop strand extension? Because if the extension is too long it won't be necessarily complimentary to the other 3' overhang.

[@patamon6475]

thanks for your effort

[@dc33333]

excellent speaker

[@ayaqz3144]

thank youu i will pray for you

[@mobin4333]

thanks alot

[@mikesmith3859]

*Knows complex science surrounding DNA repair also elliot *calls pink purple multiple times

[@mattiles5811]

how is the overhang ~1000 bp when the MRN only moved back 100-200bp?

[@Dead1M]

There will be another exonuclease, but a 5'-->3' exonuclease, which cuts away the DNA even further. (On the strand which has the 5' end facing the 'broken' end.)

[@Professorbubblegum]

bless you

[@alialviev1848]

CtIP, not CtLP. But great!

[@salibkh3208]

For everything HE explained and everything he knows I'm 1000% sure he's well aware of how it is named !!!!!! Great is not enough to describe this lecture !!!

[@sdas127]

nice illustration. Ctlp is not correct. It is actually CtIP.

[@emilynischwitz5371]

Thank you. For a second I thought I was losing it.