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In this how-to video, we'll walk you through the essential steps for cleaning the focus electrodes on your LCMS-8060NX system, specifically for Electrospray Ionization (ESI) and Dual Ionization Source (DUIS) units. Overtime the focus electrodes can become soiled showing a decrease in transport efficiency and detection sensitivity. When this is observed, cleaning of the focus electrodes will be needed with the addition of the corona needle if it is a DUIS unit.
Join us as we demonstrate a detailed, step-by-step process for safely and effectively cleaning the focus electrodes. We'll provide practical tips and best practices to ensure thorough cleaning without damaging sensitive components. Whether you're a seasoned LCMS operator or new to mass spectrometry maintenance, our comprehensive guide will empower you to perform this critical task with confidence.
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0:00 - Introduction
0:32 - Instruction Manual Reference
0:45 - Items Needed
0:59 - Remove PEEK tubing
1:09 - Remove Ionization Unit
1:13 - Retracting the ESI Capillary
1:22 - Removing the Ground Plate
1:34 - Removing the Focus Unit
1:40 - Removing Focus Electrodes 1 and 2
1:57 - Polishing the tip of the corona needle
2:03 - Cleaning SOP
2:29 - Attaching the Focus Electrodes
2:44 - Attach the Corona needle
2:03 - Mounting the Focus unit
3:16 - Mounting the Ground Plate
3:31 - Readjust ESI needle.
3:35 - Mount the ionization unit
4:00 - Reconnect LC tubing
4:11 - Conclusion