How to make a microfluidic device

[@CuriousMindCheng]

The people who made this video and decided to make it freely available to the public are saints.

[@KUMAR-bq4xn]

Great video. Very detailed and even the small details are included. Thanks for your efforts

[@nefariouscoon]

Thanks for sharing your optimized parameters...it was indeed a great video!

[@anaxprincessx]

Very informative and coherently put. Thank you so much.

[@deepakkukkar6956]

Excellent video. Thanks for sharing the details of various optimization parameters and do's and don'ts.

[@pierangelogobbo6786]

Thanks for sharing your experience guys. Excellent video!

[@RajasPoorna]

Thank you for the detailed tutorial! I am grateful! Now that I know how complicated this process is, I will try to find a different process to make my microfluidics :D

[@Chrisreynolds0724199]

You can do a very similar process with UV resin used in 3d printers. Resolution is not as good, but the process is much easier. Spin coat wafer with resin and cure under UV to make bonding layer. Then spin coat again and put mask over and expose to UV for ~30 seconds. Rinse with ethanol to remove excess then do additional UV cure for ~30 min. Then you are done! Takes about 1 hour total time. The resolution you get with UV curable resin is not as good as photoresist, but we can get 100 um resolution about.

[@sanampudasaini3379]

Excellent video. Thank you for making and sharing the video... Hoping for more videos regarding fabrications, tips, tricks etc... Bonding wire, sputter electrodes and all... Many people find binding wire to be difficult.

[@benh5336]

This is all well and good, but does it work as well as the shrinky dink method?

[@_c_e_]

I Agree. I am a noob but I think the shrinky dink method (KISS) is far easier to develop with in both time and complexity, is resistant to loses of resolution to channel widths, and, is less fraught with turmoil and danger from toxic chemicals. Superior in many other ways too! (I mean, just watch this video in full for the 101 step madness). Nonetheless, this is a very good video describing a complex set of methods to a simple problem. Kind of reminds me of the old etching days of tinkering with pcb's... Now, design in cad, send to china, jump in glee as your product returns from the tech capital of the world in a lovely cardboard box bubble wrapped and certified to the nearest thou of a micron; can totally see this moving there if it has not done so already. Very interesting field.

[@mynameisthis1580]

With shrinky dink, you're freehand drawing structures onto the chip. With PDMS (method in the video), you're designing the structures in autocad. Autocad will give much more precision, and is more easily shareable, than freehand drawing. Secondly, changing features, such as 5um channels to 6um channels is much easier to do when you're talking about autocad. In terms of costs, PDMS chips cost about ~$1 per chip.

[@Mutantcy1992]

@@_c_e_ hey if you're still into this you should check out the ESCARGO method, as it enables you to make channels in PDMS without all the procedure shown in this video. It uses 3D printed ABS.

[@samia-123-c1b]

thank you for sharing this guide with us !

[@amirsharif3913]

thanks for sharing this great video. I enjoyed watching it.

[@ronymurickan8976]

Wonderful.. Well explained

[@arafat877]

Congratulations from Morocco, you have done a great job, could you share with me the list of the machines (uv exposure, plasma, heater ...), chemical products you have used to make this microfluedic device please ? As in Morocco we don't have those things, so I should buy them from outside of Morocco, and if possible a PDF version of this video, thank you in advance.

[@물장난감]

Thank you for detail information👍

[@ozgeerguder731]

Thank you very much, science guys!

[@bd8502]

Very thorough.

[@kdm8568]

This is very helpful video! thanks a lot

[@harkamanghag6877]

Good Job Guys.

[@aunraza3427]

amazing. thanks for sharing it

[@carmeladeluca1153]

really clear.. thanks!

[@yiyangwu1446]

Thank you for sharing, it really helps a lot :)

[@李封灵]

yeh

[@pashasayyad3239]

Thank you for this valuable information. I have one question. Can you please suggest how we can integrate substrate inside the flow cell?

[@Mohamed.wael7]

Fantastic!

[@hootintsai7023]

thanks ,its very useful

[@adetoyesealbert2093]

Thank you for sharing

[@Acute.]

Thank you

[@sankargollu0911]

Nice video. Thank you for sharing. How it is different from microfluidics flexdym?

[@eliokarim6551]

Greattt 👌👌

[@micaheller4345]

What type of needles are those that you can easily attach the tubing to?

[@Mutantcy1992]

A similar kind is the butterfly needle, which is made to be fed by tube. That would require you blunting the tip though.

[@mnquispe7142]

Thank you!!!

[@ashishamlan]

hey, can i get the equipment list and its link to purchase, which are being used in this experiment?

[@makarand309]

Brilliant

[@BigBang4821]

cool vid!

[@figure35]

Thanks

[@teodygumabat4488]

Hi, thank you very much. Can the oxygen/air plasma be replaced with something er.. cheaper?

[@PBGetson]

Check this out. Worlds Smallest Tesla Valve? - Shrinky Dink (Shrink Film) Microfluidics kzbin.info/www/bejne/m3-lmJJnfLWofpI

[@Zaniahiononzenbei]

@@PBGetson Thought Emporium is an absolutely amazing channel. I'm hoping he'll try and make masters with the Shrinky-Dinks, and then produce silicone versions. He has a love for plasma, so I imagine he'll show how to do oxygen plasma bonding later on. :)

[@Chrisreynolds0724199]

Posting for others in case someone else has this question: You can use a bunsen burner flame to plasma clean the PDMS and then bond it to the slide. It is more difficult to do and you may get small amounts of carbon residue in your microfluidic, but it can be done by holding the PDMS close to the flame, but not so close it burns.

[@rohinshyam3659]

What is the name of the film used to create the photoresist Mask?

[@eyedontfollow]

mylar tympan with ink

[@iurirobert9845]

good video, but i am new at microfluidic devices so i think is not a introduction video because a few things went over my head

[@lmbt456]

What are its application???

[@WayneJohnsonZastil]

Micro fluids mainly medical precision dosages

[@CostSteam02]

how did this get in my recommended and why did i watch it at 2am how did i get here

[@improbabile]

Guariscimi tutto anche alla signorina Maria pascarelli ravaschieri fieschi del drago sono Biagio di balsamo e famiglia sono a roccapiemonte a via gargiulo vicino alla scuola elementare quella vecchia vieni avanti a benedirmi le 3 case in cui abitiamo noi di balsamo e famiglia

[@ismailerdem2060]

Why not sla 3d printer

[@kieramcadams4103]

3d printers are relatively low res

[@maravalverde5814]

@@kieramcadams4103 The 3D anycubic Photon has a 25-100 micron resolution, and it's a fairly cheap one, I found a paper to develop a device to perform RPA and they used a Viper to make their molds

[@yummytis]

Mara Valverde may i know which paper?

[@ThanhNguyen-xb9iu]

@@maravalverde5814 25-100 micron resolution is still too big for making microfluidic chip.

[@sprucescience]

Using a vacuum mixer is a great way to prevent bubbles from forming in the PDMS mixture. Check out this video kzbin.info/www/bejne/sHWwn3emerhjkLM

[@tru_710]

Where can I get the books, chemicals and equipment at a discount?

[@tru_710]

Make one device that can do everything from cooking food to spinning and vibrating things for science and sell it everywhere