How to Make Mushroom Grain Spawn

In depth breakdown on grain spawn: mothermycelium...
Grain spawn is fundamental to growing mushrooms. In this video we go through how to make high quality mushroom grain spawn from scratch. Learn how to make mushroom grain spawn that is perfectly hydrated and optimized for fast growth. Ensure your next mushroom project is a success with healthy and robust mushroom grain spawn!
Inoculation and Colonization
To inoculate the grain, inject with a liquid culture or spore syringe through the injection port. Allow your grain to colonize at room temperature (60 - 75 degrees) away from direct sunlight. After 7-10 days, your mycelium should start to take hold. When the mycelium is well established and has grown about 2-4 inches, carefully break up the grain and shake it without opening the jar or bag. Incorporating the colonized grain evenly into the uncolonized grain will significantly speed up colonization. When the grain is completely white with mycelium it is ready to use. The full process can take weeks to months depending on the species and vigor of your culture.
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Mother Mycelium is more than just another mushroom farm. We believe in the power of fungi to help regenerate and rejuvenate the health of people and planet. Rather than just sell another mushroom product, we want to provide you with the tools and knowledge to grow your own mushrooms and design your own restoration projects using fungi. We offer full spectrum growing supplies, cultures, mushroom classes, and merchandise.
Disclaimer: We are a gourmet and medicinal mushroom farm only. We do not grow, manufacture or distribute illegal substances.

[@MotherMycelium]

More info on how to grow mushrooms at home! kzbin.info/www/bejne/jWTbpKSuj6ehh5osi=sjMmYgIcm7CbRhDc

[@pearlsmaroff2782]

How many cc's of spore culture per pound of sterilized grain should I add?

[@MotherMycelium]

I add about 1-3 ml (cc's) of spore solution for 1-3lbs of grain. If I am looking for faster colonization I will add up to 10ml of clean liquid culture to a 3lb bag of grain. Thanks for watching!

[@johncoe6304]

Confused. Do we have to use the liquid culture AND the agar thing? I only inoculated my grow bag with the culture. Thanks in advance

[@MotherMycelium]

No you should only use liquid culture OR agar plates (or spores etc.) to inoculate sterile grain. You're just transferring some mycelium from one medium to another so using liquid culture and agar would be unnecessary. Sorry for the confusion!

[@johncoe6304]

It's ok brother. Total rookie here. Like your guys style!

[@AkamiChannel]

It's just two different methods! Some use agar plates and some people use syringes!

[@amiethomas1337]

What’s the best way to sterilize if you don’t have a pressure cooker

[@MotherMycelium]

Howdy! Unfortunately for making grain you really need a pressure cooker to achieve full sterilization. If you don't have access to a pressure cooker I would recommend buying sterilized grain. You can also use Uncle Ben's rice but that is not ideal for mushroom cultivation. If you're just planning on doing 1 or 2 grows, buying sterilized grain that is made for mushroom growing is the best option. If you're planning on growing mushrooms long-term buying a pressure cooker is 100% worth the investment. Let me know if you have any more questions!

[@manrawat6639]

Write in English please

[@Hezarfendir]

Great master, please add Turkish subtitles to your videos.

[@ObakuZenCenter]

Spore to grain is worst practice in mycology.

[@iliadin93]

Do you have alternative?

[@techdedicated]

🐐

[@oluwasegunsalako5034]

Hello. Such a great video ☺️. I saw some maize cob on the floor budding whitish mushroom spawn and they are everywhere. Can they be made to produce spawn or not? If yes how?

[@MotherMycelium]

Thanks for watching! I'm not sure I understand your question? If you've got dried corn kernels you can absolutely use the same method shown here to make mushroom spawn.

[@ichkaodko7020]

for grain spawn, are you using brown rice or wut?

[@MotherMycelium]

In this video I'm using hard red wheat. You can definitely use brown rice, rye, millet, corn or really and whole grain

[@mahmutvural8885]

Which method are you using?

[@MotherMycelium]

We use the second method. We use bags to make spawn which makes breaking up clumps of grain much easier. Skipping the pre-cook saves a lot of time and energy in large scale grows

[@mahmutvural8885]

Thank you

[@mooshroom3972]

Impressive setup! looking forward to your stuff!

[@flyingS0up]

Are there alternatives to sterilizing jars other than a pressure cooker? Can I put the jar in boiling water? Can I use a crockpot instead?

[@MotherMycelium]

We do not recommend it, but you can sterilize your grain jars using a pot of boiling water. You will need to increase the sterilization time quite a bit and you still risk losing some jars to contamination. If you are looking to make your own grain spawn, it is worthwhile to invest in a pressure cooker. If you are just planning on doing one or two grows, then it would make more sense to just buy grain that has been sterilized for mushroom cultivation.

[@kartalbaba981]

👏👏👏👍👍👍

[@jessie0078]

This was so helpful!

[@dcollette5411]

Where do I store the jars after inoculation? My options are in a dresser drawer, closet or on top of a table in a room.

[@MotherMycelium]

It's best to keep them out of direct sunlight in a place where they won't be disturbed that will stay around 65-75 degrees. It sounds to me like closet is your best option

[@richardmullane9279]

Hey I’m new to you and I like your video what is the best temperature for her both spawn and grow

[@MotherMycelium]

Thanks so much for watching! We generally incubate spawn at 70-75°F (21-24°C). For fruiting we lower the temp to the low 60's but the fruiting temp really depends on the species

[@paulocic7092]

Did you add gypsum to the pre-coocked grains?

[@MotherMycelium]

Yes, I sometimes add a small amount of gypsum to the water while the grains are being cooked. Gypsum is definitely not a requirement though.

[@francoisnzabonimpa3059]

Thank you to much !!!

[@MotherMycelium]

You are very welcome! More great stuff to come in the very near future

[@GoodOleNoName3]

does it matter if the spores are added right after sterilizing?

[@MotherMycelium]

Excessive heat can damage spores and mycelium so its a good idea to wait until the grain is completely cool. Thanks for watching!

[@MotherMycelium]

Please let us know if you have any more questions! We also have many people available on discord to answer any questions you might have. discord.gg/GnhWbBnKtd

[@ryanbarrett1949]

good video!

[@MotherMycelium]

Thanks for watching!