How to Pipette Challenging Liquids | Pipetting Tutorials | Ep. 5: Liquid Types

[@rong6968]

So we should spend more money for better electronics micropipettes instead of traditional ones

[@alwinkruijt8260]

Hell yes. Positive electronic pipettes are insanely precise. I did some measurements, deviations of volume on 1.6% max volume (! 20uL using a 1.25mL pipette tip) are well below 0.007mL / 0.4%. Meaning you can pipette volumes to not even use volumetric glassware (e.g. 0.1mL+24.9mL=25mL). The total deviations of all dispensed volumes summed up are still way below the deviations of the calibrated glassware. This can improve your overall measurement uncertainty by a lot. Total cost of analysis is still mostly determent by analyst hours, not by the 1EUR pipette tip.

[@nounou_k]

I need to use a micropipette to measure 600uL of sulfuric acid, can I? I'm afraid the pipette will break. I tried it once, and there was brown liquid coming out of the pipette hole.

[@Eppendorf]

Hi 김누누, thanks for your message. That doesn´t sound like it should. But with this comment you reached the social media team from Eppendorf. We can´t help you directly as we are no specialists for pipettes. But we will ask our experts and come back to your question as soon as possible.

[@Eppendorf]

Hi 김누누, for our Research plus and Reference 2 pipettes we offer chemical resistance tables on our website (www.eppendorf.com/manuals) that include sulfuric acid in varying concentrations. Pipetting sulfuric acid 95% is suitable with both of them, you just need to replace the silicone O-rings in shorter intervals than usual and we recommend using ep Dualfilter T.I.P.S. filter tips to help protecting the pipettes from aggressive fumes. If you need to pipette sulfuric acid on a regular basis, we recommend to use lower parts with higher chemical resistance (available as accessory for select Eppendorf pipettes) or - even better - our Multipette/Repeater multi-dispensers. With these, you should not have any issues since the tip design includes a hermetic seal between the liquid and the pipette. To learn more about pipetting challenging liquids and how to clean your instruments properly, make sure to also check out our webinars at www.eppendorf.com/labchannel. We hope that helps to solve the current problem? KR your team from Eppendorf