How to Submit Nucleotide Data in NCBI | SUBMISSION Step by Step

[@سميربركات-ن3س]

Thank you so much Sir for your interested and helpful video

[@elvinceager]

I cannot thank you enough. thank you very much

[@hadilvallooran123]

It was really helpful..

[@pheemmmy]

Thank you...very well explained

[@drshinwarishinwari1253]

Thank you so much sir you made my day love it 😍

[@SCIRESEARCH]

I'm glad it was useful for you!!

[@aswathyravindran6216]

Thank u so much Sir.

[@kishoreg8288]

Hi sir... How to analyse the raw genome sequence data for the submission... How to compare with the reference strain and conclude...

[@ChauhanWassi]

hello sir, can you upload a video on "How to edit/Update the annotation of already submitted Mutated Human DNA seq?"

[@NATURO458]

Please tell the steps in submit the Mango plant DNA sir🙏🙏🙏🙏

[@hadilvallooran123]

Thank you so much sir

[@waheedazizeofficial6751]

Good sir

[@NATURO458]

How to submit the mango plant DNA sequence genbank sir , please help me sir

[@nailakhanbangash2758]

Thank you for the video. It is helpful. I want to submit EF1-728F sequence for fungi. In the video you submitted ITS. So, for the Elongation factor Which option should I select? Please help me Thank you

[@Ramya.85]

What strain name I need to give sir?

[@MrFilu13]

hello sir, plz make a video on " how to submit bacterial whole genome sequence at NCBI "

[@SCIRESEARCH]

Hi Filu, okay, I'll take time to creat a video on it. Thanks!

[@mb_10_divyamori56]

Hello sir .. kya mei mera sequence submit kar sakti hu jabki mera sequence ka version number se dia hua hai ?

[@sudhamathpal5156]

I have received forward and Reverse sequences after sanger sequencing. So my question is what sequence I have to place in the nucleotide submission file? please suggest.

[@SCIRESEARCH]

You need to align or complement your F and B sequences into a single sequence and then you submit that sequence.

[@sudhamathpal5156]

@@SCIRESEARCH thank you. is there any software for that

[@SCIRESEARCH]

Yes, many, easy and simple is BioEdit. You just need to use reverse complement command to combine your both F and B sequences.

[@SCIRESEARCH]

Follow my another video how to do it in BioEdit, please.

[@manasealoo61]

Thanks mate.

[@biotechnologist4797]

Sir i want to submit 16s sequences for unknown organism . how i get organisms name required for submission? Plz

[@SCIRESEARCH]

You can determine organism name via sequence, how? You should BLAST your sequence and should find the top query sequence and the associated name with the sequence, but this method is not much authentic. For identification, first you need to perform morphology ...to know at least genus ...then BLAST and phylogeny can help you to identify species. Sometimes morphology can tell you the genus and species and combining molecular data can strengthen your identification. Hope it make sense.

[@SCIRESEARCH]

With Blast you can, at least, determine Genus of the organism. You can submit your sequence's as Genus sp.

[@biotechnologist4797]

Thank you sir..its helpful for me

[@peggyotu3954]

Thank you for the video. I have successfully submitted rbcL sequences but I am having issues submitting trnH-psbA sequences in the database, please how do I go about it?

[@jinyanliang8853]

Excuse me，I am having issues submitting rbcl sequences，please how do I go about it?

[@takwawannassi2649]

hi dr, why we submit our file not to public?

[@SCIRESEARCH]

Hi, first NCBI (genbank), etc. are public depositories so research community can read and use your sequence data. Secondly, if you think we should show our data directly to public, it's dangerous...cz of many reasons...few are...data is so huge and in big quantity you will not be able to choose the target data from diapered platforms, you need a database to preserve the data with some useful annotations to help your data distinguished and identify easily from other ppls data etc.

[@takwawannassi2649]

@@SCIRESEARCH this is my first submission, and first expercience with molecular tools , so i write an article about insect molecular identification, the article is ready and complete to sent to a journal, so do you suggest to submit sequence public or i choose a date like you do in the video thank you

[@SCIRESEARCH]

Choose a date (choose expected date you think your article can go online).

[@takwawannassi2649]

@@SCIRESEARCH i need the accession number to include it in the draft of article? so what should i do

[@SCIRESEARCH]

You submit your data and schedule it....after successful submission you will get accession number ...you can insert in your article.

[@biosphere7762]

I have Submited in the same way every time come for me message From ncbi told me there is error And i cant find the error!!! Can give you my sequnce to Submit for me

[@SCIRESEARCH]

Yes,send it to me with sequence details

[@biosphere7762]

@@SCIRESEARCH give me your mail

[@marwanmahmoodsaleh8088]

How to submit whole genome to NCBI

[@SCIRESEARCH]

Please below guide how to submit genome and what you need to provide. www.ncbi.nlm.nih.gov/genbank/genomesubmit/ Hope it will help you.

[@marwanmahmoodsaleh8088]

@@SCIRESEARCH Thanks. I know this website but there many problems when I do submission

[@SCIRESEARCH]

Email me, whether it's eukaryotic or prokaryotic genome and also WGS or Non-WGS along with problems ....I've sent you email via research gate. I'll try my best to help you in it.

[@marwanmahmoodsaleh8088]

@@SCIRESEARCH Ok. Thank you dear

[@a3tube471]

Thanks for the vedio, but it says error for my COX1

[@lopamudramandal934]

How could you fix your error? Please tell me I have also face same problem

[@Azeem-x6h]

Ye gulabi english bolny ke koa zarorat hay? Urdu main bata dety. Aur itni blir video hay. Quality accpetable nahi. "OK"