How to use autoclave | Principle, Component parts, Working

[@DrShahid-Shafi]

Thank God I found this video. The best explanation regarding this topic. The video demonstration was just awesome. You're a gem. Thank you so much for posting this.

[@thinginground5179]

Highly informative. Best video and explanation out there so far. Note: do be sure to read your manuals though folks!

[@MutegekiJohnBosco]

For sure you have made me do practical virtually 😊 with this useful tutorial

[@toddwmac]

Very helpful. One suggestion on compressing gaskets like the one on the lid is to snug then tighten the lid blocks in a star pattern like you would on an automobile wheel (1,4,6,3,5,2) being sure the 1st two blocks are as close to opposite each other on the lid as possible. This process will help eliminate potential gadget deformation that can degrade the life span of the gasket. Thanks a ton!

[@Daisy20006]

Thank you so so much for this video!! I need to give a talk about these for school and I was so confused on how the mechanism worked but you explained it all perfectly Thank you ❤

[@alisarose1407]

This is very informative but as someone who's never used one and needs to I'm quite nervous. I can't seem to find any videos on the type I have. It looks the same as yours but instead of twist knows, it has what looks like two switch-opening steam exhausts on the lid. One at left and one on right. I'm not sure how I'm supposed to position these or what to do with them. Do you have any idea? Can I send you a picture? Thank you for any help in advance.

[@keithhupp5995]

Thank you I'm sitting here waiting for the water to warm up. It takes forever! Hopefully the heating element is still good at least it's warming up. Good to know it takes an hour. I even used hot water. Does your vent valve spit water when warming up?

[@nakayimaharriet2966]

Thanks so much. I am now clear with the sterilisation using the pressure cooker

[@basukalalesar]

very helpful informative video; I want to know, do you remove water and steam both completely from vaccum valve to 0psi or only remove water after switch off autoclave to create vaccum? i experience wet gauze or cotton can you help get them dry .

[@shwetagupta-f8c]

Woahhh, cool, ur content earned u a subscriber, n u deserve it

[@vk081064]

Hi Thomas that's a great tutorial. Thanks. My autoclave doesn't have a vacuum release valve. So how do I release the vacuum. Please

[@sivagurram3269]

Great explanation and practical demonstration, Thank you for this.

[@prabhavathipoolasari4751]

U know how to do autoclave with dis

[@tumushabegerald6704]

The best tutorial for autoclave use

[@goravjain6116]

Superb presentation..evertthing is well explained

[@BIOMEDSTUDENTSSEPTEMBER22]

that was a very helpful and detailed video thanks so much

[@jksurgical6244]

Very useful demo. This video wants to translate to tamil language. Please🙏 sir. I follow serving kit❤manual. Super😂

[@tapaswinijena982]

hello! very informative video. can you please suggest what might be causing our autoclave to release pressure through the green safety valve before reaching 15psi, nearby12-13 psi?

[@omoyemiaremu4685]

Thank you so much ... God bless

[@ehud2597]

Wow! I have a question,what causes the loads after sterilisation to come out wet and what’s the solution

[@augustbadsun]

wonderful !! thank you so much

[@maxwellrejoice4039]

Thank you very much. The lecture is very practical and helpful

[@gunnarg1314]

Do you put water into bin with the tools or no?

[@dr.sarangmali9563]

THANK YOU, VERY INFORMATIVE VIDEO

[@PenglamKhuak]

Sirpa is Very rich, good explanation 👍

[@rufaronhira2956]

That was very clear sir...thank you

[@umamishra7757]

Heyyy thomas I have a doubt. What is the difference between 0psi and 14.7 psi... Because in my manual it is mentioned that at 0psi the water is at boiling temperature of 100°C.

[@umamishra7757]

Also at 14.7 psi you mentioned that the boiling temperature of water is 100•C..

[@TheSingtangpaScienceGuy]

𝙰𝚝 𝚗𝚘𝚛𝚖𝚊𝚕 𝚊𝚝𝚖𝚘𝚜𝚙𝚑𝚎𝚛𝚒𝚌 𝚙𝚛𝚎𝚜𝚜𝚞𝚛𝚎 𝚝𝚑𝚎 𝚋𝚘𝚒𝚕𝚒𝚗𝚐 𝚝𝚎𝚖𝚙𝚎𝚛𝚊𝚝𝚞𝚛𝚎 𝚒𝚜 100 𝚍𝚎𝚐𝚛𝚎𝚎 𝚌𝚎𝚕𝚜𝚒𝚞𝚜. 𝙰𝚜 𝚙𝚛𝚎𝚜𝚜𝚞𝚛𝚎 𝚒𝚗𝚌𝚛𝚎𝚊𝚜𝚎𝚜, 𝚝𝚑𝚎 𝚋𝚘𝚒𝚕𝚒𝚗𝚐 𝚝𝚎𝚖𝚙𝚎𝚛𝚊𝚝𝚞𝚛𝚎 𝚊𝚕𝚜𝚘 𝚒𝚗𝚌𝚛𝚎𝚊𝚜𝚎𝚜. 𝙰𝚝 15𝚙𝚜𝚒 , 𝚠𝚊𝚝𝚎𝚛 𝚋𝚘𝚒𝚕𝚜 𝚗𝚘𝚝 𝚊𝚝 100 𝚋𝚞𝚝 𝚊𝚝 121 𝚍𝚎𝚐𝚛𝚎𝚎𝚜.

[@butkiss536]

Thank you for this informative video.

[@omoyemiaremu4685]

How long does it take to reach the 2psi

[@wrcdbandara]

This is a great video, Thank you so much😍

[@bellaagbas]

Thanks alot....so detailed and insighing

[@AsefuAdhana]

Thank you so mach for this video

[@healthbabe2691]

Hi Thomas. Thanks for putting together this resourceful presentation. What is the purpose of the sealing process with cotton and paper wrap for empty tube, pipette, beaker, and other petri dishes? I can understand the reason for the sealing process for the flask and bottle filled with the liquid/byproducts from experiments. HB

[@TheSingtangpaScienceGuy]

If you don't seal them, germs can still enter the containers once you take them out of the autoclave after sterilization. Same reason and logic with why sterile urine cups, syringes, cotton buds etc are sold in market neatly wrapped in plastic/paper packets.

[@healthbabe2691]

@@TheSingtangpaScienceGuy Ah!!! That makes sense. Thanks for your info.

[@chizorom6930]

This was very helpful 😊

[@findinggreatness]

Thank you that was 👌 perfectly informatioal thank you

[@edithesianyomesesah5348]

Very understandable thanks very much

[@lydiaanyanga]

So helpful.. Thank you

[@thewestindianboy]

Very good info

[@R-Rpt]

Will the safety valve blow excess steam out again and again every time?

[@TheSingtangpaScienceGuy]

Hi soory for the late reply. Yes. Usually it does blow out steam every 3-5mins during sterilization.

[@R-Rpt]

@@TheSingtangpaScienceGuy It's been months since I bought the device, I've experienced all its features. Thank you.

[@Eyecaretube]

Thank you...very helpful

[@dipasingha4341]

Very helpful....

[@hanifahnamuli947]

thanks it's informative

[@harshithreddy3433]

what happens if u lift the whistle in the autoclave ?

[@sohamdatta2]

Very nice.

[@AmouriBecem]

Very good video. Thank you

[@rathimeena8524]

After 1whilstle should we wait for 20mins ?

[@TheSingtangpaScienceGuy]

once the guaze reaches 15psi, start the 20minutes timer. Not necessarily the first whistle

[@umamishra7757]

Please make a video on hot air oven

[@TheSingtangpaScienceGuy]

Sure. Will try

[@EVANSNTSOR]

How do prevent regular burning of the element in the chamber??

[@mareflare8252]

Thnk u so much for this video

[@sksingh462]

Please mension the sutable autoclave drum size to put inside the autockave

[@TheSingtangpaScienceGuy]

12inches autoclave diameter, so anything less than 12in dia will fit.

[@eliasody2912]

Thank you

[@rathimeena8524]

Will it come to 0psi automatically

[@TheSingtangpaScienceGuy]

yes it will, as the system cools down to room temp

[@kandykane630ify]

Can you adjust to stay above 17 psi?

[@TheSingtangpaScienceGuy]

Maybe close the 15psi safety valve completely?

[@fahimabdikadir9919]

Hii kindly I wana to write a lab report on autoclaving kindly help me on how to write

[@biswajitdutta4161]

Where was connect the sensor wire ?

[@sksingh462]

The loaded tray size

[@sunithabv9529]

Thank q ❤️🥰

[@catlynshii8936]

Woow

[@shalomiperera3528]

The gauze gets wet

[@shafiquerashid234]

Can we adjust pressure???

[@SchoolSchoolSchoolSchoolSchool]

thank you for this advice thomastktungaung