Genuinely, one of the most helpful videos I've ever seen. Thank you so much. Instantly subscribed, and am excited to view your other videos.
@CanYouBio2 жыл бұрын
Thank you so much! I would like to do an update video on my research soon. 🙏🏼
@sadullaabdullaev54512 жыл бұрын
Mr Adrian I Pascu I am working breeding programm of seedless grapes and your skills will increase grape export potential in Central Asia! Thank a lot
@CanYouBio2 жыл бұрын
I am happy if you find my videos useful somehow! Best of luck with the grapes. I’m trying to do a gene therapy. :) p.S check out the group Petalsmyths Plant Engineering Research Party on facebook. Those guys are also in plant bioengineering (independent researchers).
@Islamic_insight552 Жыл бұрын
Seedless grapes are not good ,BCz for next grape plant it can create problem ,BCz no seed available😅 for next progeny😊
@danielsivil32736 ай бұрын
Amazing video!! you just saved my cloning project!!! (Also cute cat)
@CanYouBio6 ай бұрын
Thanks so much for the comment! Be sure to watch my next video. Testing species in meats (e.g beef, pork, chicken, sheep etc). It took a lot of time and resources to get this! :) The kitty is all grown up and freaking FAT now! :(((
@aishwaryagaikwad9035 Жыл бұрын
It was a very informative and easy-to-understand video. Thank you!
@CanYouBio Жыл бұрын
Highly appreciate the comment! Thanks!
@JasleenKaur-lu5or2 жыл бұрын
Awesome video! Finally someone who explains every small detail. Thank you sir!
@CanYouBio2 жыл бұрын
Thank you so much!
@gowthamir85625 ай бұрын
Is it possible to do the cloning without using restriction enzymes here? As in I want to insert a gene by replacing a specific in order to decide the size of amplicon.
@CanYouBio5 ай бұрын
I don't know if it's possible to simulate in snap gene. For non-enzime cloning I would suggest PIPE assembly. bitesizebio.com/23889/polymerase-incomplete-primer-extension-pipe-cloning-method/. David Ishee has an awesome video on this :)
@tonycadiz8912 жыл бұрын
Very helpful!! Thank you!!
@CanYouBio2 жыл бұрын
Thanks for the comment! Appreciate it! Check out my other videos. You might find those useful too! Have a nice day! 🙏🏼
@rakeshde15712 жыл бұрын
please make video on gibson ,topo ta cloning ..how to delete gene..please sir..please..really helpful for me..awesome video.full of knowledge
@CanYouBio2 жыл бұрын
Hello Rakesh! Thank you so much for your feedback! I will try my best to make these videos. Until then, I would highly suggest an alternative to Gibson. It's called Golden Gate. I use a slight modification of it and I do have a video explaining in detail how I do it. kzbin.info/www/bejne/b5OUh2V-gM2aa6M. I have successfully assembled like 20 plasmids using this. If you want to try it out I can help you a bit with some infos. Add me on facebook...Adrian Ionut Pascu. I don't really use gibson on TA cloning to be honest. For simple cloning of one or two fragments I would also suggest P.I.P.E assembly (it makes use of the imperfection of PCR) Polymerase Incomplete Primer Extension. Here is a link for it. David Ishee. odysee.com/@DavidIshee:d/dna-pipe-assembly-method:4 . Hope this helps :)
@user-mi1sl3ii5i9 ай бұрын
Love it! And your cat!
@CanYouBio9 ай бұрын
Thanks for the comment. U can also watch my gene therapy project update. Just finished 2 of the mammalian plasmid vectors and I will post an update very soon!
@user-ny2ck6qi2o2 жыл бұрын
OMG thanks a lot for your job you've helped me so much THANK YOU
@CanYouBio2 жыл бұрын
Don't mention it. Thanks for the comment. Also, if you've followed my other videos I use snapgene as my go to program for my gene therapy project. I kinda simulate basically everything. From pcr, gel to golden gate assembly.
@hasnaalashi24479 ай бұрын
Wonderful !!
@CanYouBio9 ай бұрын
Thank you so much for the feedback!
@jedn6488 Жыл бұрын
With the primer aren’t you getting a sequence of dna that is not the gene in the new plasmid? Can you just add restriction sites and primers that introduce new bases in vectors that will be introduced in a genome?
@CanYouBio Жыл бұрын
Yeah u can. I do this all the time. In the portion that does not anneal i put restriction sites. For example u can check my Golden Gate Assembly video. I’ve recently published a paper in which i built 21 plasmids from scratch. I have all the primers used there in additional info.