HPLC Software Tips: Diagnostic Tools

[@bsadeghi1098]

Thats very great,thanks for these useful points,could you please explain the thermostat and autosampler parts too?how can i understand the effeciency of these two parts

[@MrScorpioAngel]

very useful points to know!

[@srirampmna135]

Could u pls explain how to setup peak purity setup in spectra option in agilent

[@bezerker2023]

Can you do a sequence table and go through calibration levels such as 6 calibration levels

[@axionlabs]

Hi John, thanks for your request. Check out our recent video on how to build a calibration curve. kzbin.info/www/bejne/pmLUf2Opr9qkoMU

[@sjn7220]

LOL I don’t think I’ve ever heard someone say chemstation is their favorite 😀. I’ve been using it for 25 years and while clunky, is does get the job done. OpenLabs is slightly better but I do prefer other manufacturers software.

[@greg007gk]

You are very nice to listen to, you are persuasive, but I think you are measuring the energy of the lamp wrong. The measurement should be made with the cell placed in the detector and the aqueous liquid phase! When you remove the cell, you look at the lamp in the room through the open door, when you should be looking through the keyhole, :-). The energy of the D2 lamp with use always decreases the most from the side of the shortest lengths and additionally ultraviolet light degrades the mirror surface more than visible light. Therefore, at some point, with a given detector, it is no longer possible to perform analyzes for the length of approx. 200 nm (large noise, small signal), and for e.g. 250 nm you can work for a few more years.