HUGE Contamination Problem This Summer

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[@stealz5000]

I'm not in the mycelium-business but in my field of work hygiene plays an important role. we usually have nested rooms where air particle count is reduced step by step. This technique could be useful for you if you either put your labs inside a larger tent that prefilters the air (probably overkill, I admit) or add some kind of pre-filter to your hepa filters so the large particles get taken out beforehand. If you have enough sucction you might even be able to use a cyclone system which is very cost effective during operation.

[@jeffreysmikle672]

The resilience you have is great. Even when anticipating contamination I know it still hits hard. Keep on pushing man !

[@topflightenglish5272]

You want to buy some Bentonite. I once read about an experiment, in which some guys sprayed a hospital wall with Bentonite mixed with water. Another wall was NOT sprayed with bentonite. Over two years they monitored the bacteria levels on each wall. The wall they had sprayed with Bentonite was bacteria free - after two years!!! Maybe you can use this and spray it onto the insides of your tunnels, the shelving and also rinse out your bags with it. Check it is food grade, however.

[@mmbcook]

Bentonite Clay? Cat litter? Interesting.

[@BlueJersey112]

I think the biggest issue is that your Lab is right next to the front door so all of the spores from outside can be blown or carried inside whenever entering/exiting. There seriously needs to be a means of isolating the area where you do your lab work and the entryway of the shed.

[@pacificnorthwestfreshmushr930]

That's a huge loss he had. I wouldn't have made a video about it.

[@dujour79]

I hate to hear this guys!! That being said, I am do glad you show what's going on and give an in-depth explanation of how you are going about finding the source of the problems, what you're doing to remedy it and what your plan is moving forward. This is such great information for anyone either starting out or wanting to better their business. So, sorry for the setback but keep up the great work! We love your channel and can't wait to see how things move along!

[@63phillip]

A quick way to sterilize rooms, jars, bags, and all things ( except for live Mycelium grain spawn) is to use UVC light.

[@chasal2533]

the great thing about the hardwood pellets is that they are pre pasturized. you may get away with less sterilizing time therefore somewhat quicker cool down time. every time i mess with my bags when they are still hot i get problems

[@busyrand]

Thanks for sharing your thoughts on this. Very fascinating....

[@emartin8907]

i thought i was the only one throwing out 100% of my bags to contamination, thanks for sharing! never give up!

[@andrewbarlow8937]

Education. Thank you for your videos.

[@Commander4AL]

I load my unicorn bags in the 55 gallon steamer with the folded side down run it for 18 hours and unplug it. I throw on some welding gloves and unload after 10-20 min cool down. I am using 50/50 soy hulls and sawdust. 7lbs of substrate per bag. I have to watch when I am loading that I don't dump a bag out but after the sterilization run the bags are pretty much vacuum sealed when I pull them out.

[@jamesJohnson-qe4gw]

trich is gold for gardeners

[@rjaquaponics9266]

Just an idea, build air locks around doors. As you walk in, the outside air is exchanging so the air cleaners are being bypassed. Air locks will prevent air exchange when entering/exiting.

[@pacificnorthwestfreshmushr930]

If your quick and sloppy infront of your flow hood air locks aren't the answer. A chip fab lab is cool when you can operate it in the correct way.

[@rjaquaponics9266]

@@pacificnorthwestfreshmushr930 Airlock space must be more than 1 step wide. Open outside door, close outside door, walk two or three steps to open inside door to enter lab. It was just a thought. I implemented such an airlock design on the front of my 20' geodesic dome. I had 12' airlock where I stored wood. The inner and outside doors were never open at the same time which prevented air exchange.

[@c12onnor]

I tend to use Unicorn bags with T filters for grain spawn runs, then transfer under clean laminar flow to master's mix using A filter bags for a bit more breathability, pore size is still too small for most foreign fungi spores to enter the sterile bag. Most contamination issues is due to the grain to bulk substrate transfer, still air in a clean room with the laminar flow HEPA filter running for an hour or two should completely clean the room of spores and dust. Otherwise it could be the sterilisation process, best to use heat under pressure e.g. pressure cooker/autoclave, or a barrel autoclave for large scale that runs for 24 hours. You can also get the built in injection port Unicorn bags which reduce contamination risk and can even be inoculated with LC out of laminar flow.

[@samb202]

“We decided to test 350 bags”. Damn. 350? Y’all are ballin.

[@MarthaMacArthur]

What do you do with all your plastic waste?

[@jasonclements4607]

Bags pull air while cooling down right?

[@ExploreAroundUsOfficial]

What type of contam was it?

[@clubadv]

Feeling better about my summer contamination now.

[@melparadise7378]

I'm learning to grow right now. I brought in an oyster mushroom from the grocery store that happened to be harboring orange mold. Lost half my grows. :*) still trying.

[@Aquavidify]

Could there be a role for Colloidal Silver as a natural bacteriocidal in mushroom cultivation? It's very cheap and easy to make.

[@asamusicdude]

Or h202 when pasteurizing?

[@colemankamryn]

I work in the biotechnology field. We grow virus, cells, and bacterial cultures that ultimately are processed into antigens and vaccines. In our class A labs we alternate between disinfectants weekly and monthly. Try using Spor-klenz once a week in order to kill those hard to kill spore forming bacteria and other unwanted mold spores.

[@whatthefungus]

ColeKam will look it up. Was thinking about fogging our labs once a month with hydrogen peroxide or bleach

[@geekswithfeet9137]

I’m my home lab I will put on a 24hr cycle of ozone that gets up to 4000ppm ozone, then I switch on a 265nm UV flood light, that adds a second layer of sterilisation but also neutralises the ozone. After that I leave an agar dish out for 10 minutes uncovered to survey, literally not even one CFU detected after a fresh cycle so far

[@cakopinolo]

Hello WTF people...sucks when this happens at your work!!!tek question;do u make your miscelium??and if do how u do it,with agar discs,seringe liquid spores???thx a lot

[@johngrady1755]

If you watch more videos youll get these answers

[@jasonblair1114]

Hi again, Brian! Word of advice: All you have to do is pop the lids on your drums off and leave the bags in there for a 24 hour period. Then they've cooled off enough to where you can safely move them into your lab and leave them for another 24 hours to further cool. Then inoculate. It should not be necessary to seal the bags before you sterilize them. Keep up the great work man!

[@whatthefungus]

J Blair that's what we do now.. but the fuel pellet bags are way to hot still after 24 hour cool down

[@jasonblair1114]

Dang! Surprised they're still that hot 24 hours later. Must be pretty flipping hot outside up there right now. Well let us know how sealing then sterilizing works out for you, and good luck!

[@until11]

WHAT THE FUNGUS Noob question, but why can't you run cool water through the sterilizer for a few hours prior to handling?

[@johngrady1755]

I believe the bags are not sealed, which would be a problem. Secondly, they have a filter patch which would allow water intake. Besides those two things, you have to use water, which is more money/work than simply waiting 24 hrs.

[@mollyypollito6083]

WHAT THE FUNGUS

[@bthegardner2213]

I immediately started adding up the cost of loss in the truck bed. I feel your pain. Why don't yall use liquid culture to inoc bags so no sealing cutting inoc. and resealing? Is it for less steps or so no puncture in bag to have to worry about contamination point? Costs more too i guess.

[@ambalapetlover7904]

👌

[@jordanmercier3616]

Liquid cultures are just too inconsistent for commercial operations.

[@TheManInCommand]

4:35 I don't understand what he means. Is he saying that he's going to take the bags that are in the bed of his truck and resterilize them so he doesn't have to compost them? @WHAT THE FUNGUS

[@zleereed]

wonder if you can add a filter to your prefilter. even nylon stocking. Anything to get the bigger particulates out of touching the prefilter.

[@forwhatitsworth364]

Free HACCP lesson right here.

[@Quilbo]

Good videos man I just can't understand some words. What's in the bags? 50% hardwood pellets sawdust and the rest? "soy haw" ? What's that?

[@whatthefungus]

QuilboTLG soy hulls

[@devanvelo3725]

What contaminates it? Is it another fungus? What type of fungus?

[@whatthefungus]

Devan Velo black mold or trichoderma

[@johngrady1755]

I'm not sure what the specific culprit is here, but I think I understand it's almost certainly one of a handful of potential baddies. If you're interested to learn about contams, here is a link with top notch info on all the notorious villains. Look under 'diseases' americanmushroom.org/integrated-pest-management-2/

[@ringodooby]

Just like Myers mushrooms does it

[@tylerm1538]

Love the video, just turn down the intro music bros! :D

[@OpiumWalls]

This is like a vlog parody song

[@noyezz3690]

hey I've got a rather interesting question ... well people feel the need to say "what the fuck" do you feel the need to say " what the fungus" ? :D

[@ahlay5306]

😂 😢😢

[@pacificnorthwestfreshmushr930]

Damn. Lost the whole farm lol. Contams happen but that's just insane. If you didn't start out in a sab and jumped infront a flow hood from the get go you did yourself a major disservice. Its a good video but it doesn't tell the whole tale. Forest fires aren't the issue. I personally believe every grower needs to start off in an sab. Your movements are constructed in an sab. I think this is an example of what cutting corners can look like. Its a business but the mushrooms don't know that and your flow hood doesn't know that either. Do an sab video. Doing the wrong things for a long time over and over again results in the same things. Everything down to the movement to where tools are positioned infront of a flow hood or sab is what I've learned. Jars and sabs should be a way every commercial grower starts. I don't think I've heard you speak much on still air boxes or what goes on inside one. Those critical steps determine the outcome of your farm and how it will operate in the future. I wouldn't advise to anyone looking to do this to take out a massive loan to go big or go home.