IDvet tutorial: How to perform an ELISA test (protocol for poultry testing)

[@Candy-sheikh]

Thank you so much for the video 🙏

[@GaryHField]

The acronyms of the test is LAM ELISA. That’s very strange.

[@lakshmi65432]

Mam what is the. Name of this test u did HIV or HBSAg (or) VDRL (or) HCV please say mam But mam the video was same as our teacher said to us thank you mam

[@zeeshanraza7423]

WHEN we use different strips of plate ...negativ and positiv controlls are used at each step

[@hassanalghalebi528]

different for same diseaes or another diseases

[@InnovativeDiagnostics]

Hello, sorry for the late reply. Please send your question to [e-mail]: our technical support team will be pleased to help.

[@kirankahal4754]

Thank u so much for this

[@InnovativeDiagnostics]

And thank you very much for your interest!

[@divasan9281]

Which insturment are you using to wash the wells? Around the 5th minute. Thanks

[@hassanalghalebi528]

you need washer to do that

[@InnovativeDiagnostics]

Hello, sorry for the late reply. There are several possibilities to wash the wells. Please contact [e-mail] to ask for more information.

[@samarwalid5038]

What's the reason of high result of control positive?

[@nextagro]

shouldn't it have done that in a sterile conditions under a laminar flow?

[@InnovativeDiagnostics]

In most cases, ELISA testing does not have to be performed under sterile conditions or under a laminar flow. In some countries, however, there is a regulatory requirement to perform Avian Influenza ELISA tests in level 2 or 3 containment laboratories.

[@hassanmahe8708]

Thank you very much very interesting

[@InnovativeDiagnostics]

Thank you very much for watching us and for this nice comment, very appreciated!

[@zahraafakih7020]

What is the internal reference material used for? Like what's the purpose..?

[@InnovativeDiagnostics]

Hello, sorry for the late reply. Please contact [e-mail]: our technical support team will be pleased to answer your questions and give you more details about the reference material.

[@NawalArsalane]

I use R18

[@dvmgeelle5520]

Lets share us calculation formula of Wash solution and Buffer

[@InnovativeDiagnostics]

Hello, sorry for the late reply. Please feel free to contact [e-mail] to learn more about the calculation formula.

[@iammrm6]

Is this a direct ELISA?

[@InnovativeDiagnostics]

Hello, the test used in the video is an indirect ELISA.

[@sozanhassanein5641]

How to calculate limit of detection for compitative idvet pprv elisa

[@InnovativeDiagnostics]

Dear Sozan, please send your question to our technical support team, who will be pleased to assist you: [e-mail]. Wishing you a great day!

[@saranomrosy7821]

Howl can I get software? I get kit but I haven't software

[@InnovativeDiagnostics]

Thank you very much for your interest. To request the software, please write to: [[e-mail]]. We will be pleased to assist you in installing and using the software.

[@saranomrosy7821]

@@InnovativeDiagnostics thanks alot because I have more than one kit but unfortunately I can't get software

[@amelkanani3570]

I want to ask... Some well has more color (deep in color) than the positive control?

[@InnovativeDiagnostics]

Hello Amel, With indirect ELISAs, the more a sample is positive (containing more antibodies), the stronger its color will be during the revelation step. A sample may contain more antibodies than the positive control. Consequently, this sample will have a stronger color than the positive control.

[@amrosalman5411]

Thanks alot

[@InnovativeDiagnostics]

You are very welcome!

[@ThịĐạoMai-d1h]

Tôi thích sản phẩm này ❤❤❤

[@InnovativeDiagnostics]

Thank you so much for the nice feedback! Cảm ơn bạn rất nhiều ;)

[@shivaprasad6493]

Sir hiv elisha test refar rann 0.2127 nagative positive please respond any where

[@InnovativeDiagnostics]

Hello, you may send your questions to [[e-mail]]. Our technical support team will be pleased to assist you in your result interpretation.

[@shivaprasad6493]

@@InnovativeDiagnostics contact no

[@amandabarthes6953]

@@shivaprasad6493 You will find our contact info here: www.innovative-diagnostics.com/contact-us/

[@sallymusallam2178]

I'm also in for some checkups ELISA refar rann 0.15 negative positive ! In your opinion what is the problem and what is the solution !?

[@moussaeshra6212]

I didn't understand any You are talking as we know all the basics We dont How did you prepare the sample

[@InnovativeDiagnostics]

Thank you for your interest. This video aims to describe the main steps of a poultry indirect ELISA protocol. If you need any additional information, for example on how to prepare your samples, please feel free to contact us at [[e-mail]]. We will be pleased to assist you.

[@IbrahimAhmed-ht7rc]

😄😄😄😄🌹🌹

[@AndishaAlex]

Sorry, in Amharic

[@InnovativeDiagnostics]

Hello, you may activate the subtitle option in the toolbar at the bottom of the video. An automated translation is available in various languages, including Amharic.