Illumina sequencing | DNA sequencing by synthesis

[@Ametsi117]

The so called professors or doctors at universities are just doing the job for living, not coz they have passion for science. Therefore, they always fail to communicate science in a simple way as you do brother. Much appreciation! you are making huge difference.

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@priyanshu95.]

​@@shomusbiologyofficial❤😊

[@bishalgurung776]

I am doing MSc Biotechnology in UK but still i look up to your videos which makes easier also time saving .I hope one day i would make you proud sir 🌻Thank you so much for being an inspiration

[@shomusbiologyofficial]

Glad to help you

[@Nomanenamememe]

I am just a High school student learning this😅

[@pagalump5265]

From which uni you are dping MSC biotech????

[@nowshinjahan7502]

I really don't have sufficient words to express how grateful I am! you have made biochemistry and molecular biology easier to learn. thank you so much.

[@somnathbanerjee4873]

Shomus Biology Way Beyond His Time What Other Institutes are teaching today that was already taught way back in 2015 😮

[@UmerKhan-kd7us]

thank u sir when i am unable to understand any topic i opened your videos and and i get solution of my problem there .you are very intelligent and way of teaching is mindblowing

[@shomusbiologyofficial]

+Umer Khan thank you. I am glad that you are getting benefit from the videos

[@BRENNANSTECHBITE]

Can you make a video on epigenetics, bisulfite conversion, methylation-specific PCR, and bisulfite sequencing? Thanks!

[@cellbioinfo]

Thank You so much bro... You are so good all the way... Proud to be Bioinformatitian... Love You...

[@adekunlemaryam1580]

The way you explain these things, it makes it easy to understand

[@bbeecckkyyss]

thank you for this video, it is the best explanation of Illumina sequencing we can get!!!

[@christinejackson4209]

Thank you for the excellent explanation of the bridge amplification method.

[@darkestnbl]

the primordial shomuji

[@sumitkumar-el3kc]

Thank you so much for making concepts understandable.

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@humairahr3401]

Thankyou Shomu this is the best explanation :)

[@shomusbiologyofficial]

+Humairah Rashad you're welcome

[@viniekouamou8188]

you ARE SIMPLY THE BEST

[@shomusbiologyofficial]

+Vinie Kouamou thank you. Glad you liked my lectures

[@viniekouamou8188]

please keep it up!

[@Gio-zc3zn]

Indians are the best!! thanks dude

[@shomusbiologyofficial]

+Francisco De Francischis you're welcome

[@kiranakhtar7788]

Very well explained, thanks

[@shomusbiologyofficial]

You're welcome

[@poonamvijaypuriya8815]

Thank u very much sir . U made this topic very easy to understand.

[@jamalinoor2411]

Excellent explanation, thank you shomu

[@shaswatadutta8671]

Sir is there any recommended book to read sequencing? Thank you so much for the explanation

[@zahraaamjad2952]

Loved this video!

[@shomusbiologyofficial]

Thank you

[@asuramunikavishadesilva371]

Thank You! ❤

[@shomusbiologyofficial]

You're welcome

[@naomigu8131]

Are different fragments amplified on the same slide?

[@nchimunyamuloongo4436]

Awesome explanation

[@shomusbiologyofficial]

Thank you

[@okpolihenry983]

WHY DO WE FRGAMENT D DNA BEFORE SEQUENCING THEM? AND CAN WE ALSO USE SONICATION METHOD TO DO SO ?

[@shubhisingh3663]

There is limit to reading the base pairs . No machine can read it all once

[@farsanaa7261]

Great explanation👍🏻

[@shomusbiologyofficial]

Thank you

[@melanieferrace7484]

Thank you Shomu ! Excellent explanation, so helpful as always :D

[@tjtbkgaming]

can you please make a video on PacBio sequencing? i couldn't find it on your channel

[@arnitheking]

Hi, can you explain to me why we need 2 reads when sequencing? Also, is barcoding necessary when analyzing self "made" (PCR) 16s rRNA gene fragments? or is it only useful when you get samples from libraries?

[@nevroth]

I'm still trying to figure this all out myself, but for the barcoding question, I think it's only necessary for high throughput or when you don't know exactly what you have. I don't know what you mean by self made, but if you clone a 16s fragment for identification you can send it for sanger sequencing which is much cheaper

[@farheenkhanam3166]

thank u sooooooo much sir......for this vidio .it is really very helpful to me

[@jayachakraborty1517]

very good explaination sir..

[@brendaluhanga4190]

does bridge amplification produce a duplicate or a compliment sequce??

[@sonytambattilsonytambattil3673]

Sir can you upload a vedio regarding the relaxation of sequnece reads in bioinformatics?

[@pabitro1]

How will be the loci which have 50% probability to have two of any base (like you explained) be resolved? stated other way round, is that a limitation that Illumina have or can it be resolved by resequencing or Gap joining algorithms?

[@gayathria.s7073]

Why adapters are added on both sides of the dna fragment? Is it for bridge amplification?

[@jacquelinelabovitz4613]

SHOMU! you are amazing! keep up the good work

[@sidratulmuntaha1687]

In the bridge dissociation step....what happens to the stranded DNA that it dissociates and becomes single stranded? and secondly...Why one end of the strand remains attached while the other is free? Why don't both the ends tend to become free altogether? Kindly explain it...thanks!!!

[@mansisart2124]

Thank u....ur best ❤️

[@shomusbiologyofficial]

Thank you

[@AyazSamo]

Love you shoumu !

[@Hanan-wq1op]

Thank you sir very clear

[@shomusbiologyofficial]

You're welcome

[@sylviajimenez1059]

Thank you so much. Lifesaver!!

[@shomusbiologyofficial]

+Sylvia Jiménez glad I can help

[@a.alshamiry7033]

What the different between Next generation and sanger and when i used this two ??

[@siddharthadas86]

how does the denaturing ensure that each of the type of ends will be attached to the surface and other end will be free? I mean why not both violet coloured ends be attached and both pink coloured ends be free? why does it have to be one of each? Can you please clarify?

[@veena6586]

Does this pcr occurs in iso thermic condition?? Its not like normal pcr

[@sanjoylouha6159]

can you please refer a book for studying all type of sequencing method?

[@rizwanasyed14]

now a days, data we will get from company but after getting NGS data (illumina)how to its a big problem, which software we have to yse and how, can you tell?

[@md.ridwanahmed1769]

Can i get those images you are using in this video of illumina sequencing???

[@myra9250]

ThankYou.

[@shomusbiologyofficial]

You're welcome

[@mirihansamir4118]

hi sir i hope you see this, i am very grateful for your videos they have helped me immensely those past few years. i have a question,i cant grasp why there is an overlapping in the final sequenced product. didn't we fragment the whole genome at the beginning? so what causes those overlaps? thank you so much

[@shomusbiologyofficial]

Fragmenting genome randomly generates the overlaps

[@safakhan2656]

What makes the DNA bend ??

[@Hoxgene]

Sehr Gut. vielen dank!

[@stevenlovesscience]

Another great video!

[@salehalzayer1091]

Well explained

[@proneyex]

Hello Sir. Thank you

[@shomusbiologyofficial]

You're welcome

[@annieasghar2587]

I have one question... your DNA is sequenced by ilumina miseq is of 5MB size. U have to get 10x coverage . How much data do u need?

[@senada5959]

very good

[@Ammakodumai]

Hi Can i please request you to do a video on ion torrent sequencing plz plz

[@adilrahim1454]

thank u . . . . . for making this.. . . . adil rahim abulwalikhan uni mardan kpk pakistan

[@shomusbiologyofficial]

+Adil Rahim glad to hear that

[@jayalakshmi-sc4io]

what is the nature of adapters?

[@abantikabiswas5624]

A video on SOLiD sequencing please

[@shomusbiologyofficial]

Okay

[@muhammadhanif6589]

Nice

[@shomusbiologyofficial]

Thank you

[@nailaimtiaz3617]

I didn't get the point once it bend down then how it straightend later

[@manojca11]

Please make the photos clear.. all of your explanations will be based on that pics only.. so that one can easy follow you... And I think little more information should be included on reads...

[@saramalik5440]

Breeege.... iiiis, u dnt understand why do we do in reverse and forward strand and why do u it

[@nailaimtiaz3617]

SOLiD seq plzzzzz

[@shomusbiologyofficial]

Okay

[@kaushikiupadhyay2709]

Nothing is visible sir

[@hopeislife274]

Who watching in 2024 after like me 😂

[@shomusbiologyofficial]

All the best

[@athirah1619]

Subtitles don't match with what you say

[@manishadhikari5789]

Anyone from KU?😂