Inside a CRISPR Lab

[@huntingkc1]

So whomever did the recording and editing did a great job

[@zqxzqxzqx1]

I've been fascinated by CRISPR Cas-9 since it's inception. I follow all the news I can get, and have read Code Breaker (which was more interesting and informative than I expected!) I'm excited to see it already restoring sight and correcting sickle cell. Thanks for this look into a lab.

[@peters7278]

Thank you for the video! On a side note, I think this process would be an great candidate for automation. It seems that the human has to complete soooo many steps...

[@LarsLarsen77]

It is automated, they're "teaching" people to do it for $100,000 a year, which those people somehow think benefits them, but really they're just doing what a robot already does.

[@NiTeLightYears]

@@LarsLarsen77yay finally an intellectual

[@NiTeLightYears]

@@LarsLarsen77this is cool may I know what are some complex stuff that wont be automated in years to come in yo perspctve?

[@farahbourek2120]

Can you please give me the details about methods of extraction that you use to get crispr cas 9

[@sandeepgolui8129]

Excellent to see...thanks for sharing madam

[@jibraankadri]

very well filmed and documented. great job.

[@farahbourek2120]

Please can you give me the methods of extraction that you use to get crispr cas 9. Thanks

[@SixOhFive]

You can buy a plasmid with pcas plasmid that has antibiotic resistance gene and cas9 gene

[@Aztorak_1]

Now to unveil my superior alternative: CRUNCHR. In all seriousness though this is excellent.

[@mockondo3011]

Does this lab sell the purified Cas9 protein? Can it remain active through several days of shipping?

[@eukaryotic0703]

How is the guide RNA made? does it have any special feature or is it just extracted mRNA?

[@SixOhFive]

I did crispr in my bio class recently we turned a white colored yeast pink

[@leo_amorim]

Excelent job on this video. I was studying crispr theoretical part and this video helped me to understand it pretty well. Thanks!!

[@ghulamrasool206]

Fascinating

[@bharat1031]

cool lab

[@mariadelcarmenmendoza8721]

Great video!!! She's so sweeet :3

[@danlaw1208]

Cool job ever

[@mrnerd73]

Thank you for the video ❤️ Learned a lot... Liked your way of presentation

[@ferryjap5515]

Can i order CRISPR and deliver to out of your country?

[@kashifkashif427]

I have never seen such lab

[@Tina-bo1sf]

I thought I saw a comment saying this but couldn’t find it. Could you PLEASE figure out the code sequence for the vaccine, then reverse it and fix us??? I’m in California. I’ll be knocking on your door soon. I was 49 when I took it. I’m 51 now. I had heart pain, dizziness, almost passing out and no more periods. Please fix us.

[@nathanielyoundt4936]

The sequence for the mRNA vaccine is in the public domain. No need to decrypt it 👍🏼.

[@asnakegetachew]

Great!

[@justanotherguyful]

It doesnt actually explain the entire process. A lot of the stuff theyre doing looks like it can be done in anyones garage. Very simple process its just that no one has a video actually showing the real lab work from start to finish. If someone made a 20 minute video actually detailing everything from start to finish then anyone could be a genetic engineer if they purchased the right tools.🤓

[@volution1160]

anyone can. purchase the books, learn online, buy the tools, do the experiement, coalese findings in scientific paper, and if it gets published Id say noone would argue you are not a scientist. Prestige, social hierachy, elitsm etc. are why you would probably run into more problems than someone employed at an institution doing exactly what you do in your garage.

[@kazekagekid]

@@volution1160tbh I’m more interested in knowing how they’re so certain they won’t introduce their target sequence backwards to the cas9. Do they always have to find the RNA from elsewhere or are we flying actual base pairs together at scale with some sort of machine? the sheer number of molecules in every drop, and the random motion of those molecules within the swirling medium feels like way too much uncertainty.

[@LarsLarsen77]

Newsflash: You can do crispr in your garage. Nobody's even checking if you know what you're doing or not. (unless you're in europe, then it's super illegal)

[@LarsLarsen77]

@@kazekagekid It doesn't do it backwards because the guide RNA only binds to the sequence in one direction. You design it to match the target sequence and no others, but sometimes it will make a mistake if you designed it poorly.

[@LarsLarsen77]

@@kazekagekid And yes you have to give it a target sequence (already in the right order) to stitch in, or else it will just stick whatever is around in there, which will not be what you wanted.

[@kavithaalapati9850]

Useful and informative video

[@shin-ishikiri-no]

I get that this can be used for life-saving gene modification, but it can be much more than that.

[@엄기원-f7v]

저도 여기서 연구하고 싶어요

[@griggsentertainment6463]

What city is the Lab in?

[@joyaborthakur9407]

Please Scientist 🙏 Try to reverse the biological aging process in humans.. And also to eliminate age related diseases..

[@AsuraVeri]

Why? I see no good coming out of it.

[@FahimHoq]

​@@AsuraVeriwhy not

[@Ghostfuckyou2]

Nmn pills look them up

[@JohnDoe-ml1ui]

Don't judge me.... i don't know what she is doing but that "tube scraping" at 3:32 doesn't look professional made at all 🤣

[@MrLovemydog12]

She is trying to break up the pellet at the bottom of the tube. These cells want to stick together, therefore the small vibrations help break up the clusters of cells. Flick mixing such a viscous solution of cells will not properly mix, but pipette mixing (and vortexing) could damage the cells. Eukaryotic cells are much larger than bacteria and must be treated with care, using the grate to vibrate the cells is actually pretty genius if you ask me.

[@JohnDoe-ml1ui]

@@MrLovemydog12 Yeah i know but... I mean... still don't look professional 😂 they should make a machine or something made on purpose to do that if it's something needed...

[@AdrianDucao]

@@JohnDoe-ml1ui lol you mean you need a machine that just flicks a tube couple of times? What a waste of time and energy...

[@franklinlingga5491]

@@JohnDoe-ml1ui what machine for that simple work? wasted

[@SixOhFive]

It’s called agitation

[@sandeepgolui8129]

🇮🇳👍🙏🙏👍🇮🇳🧬🧬🧬🧬🌐🌐🇮🇳

[@saltandpepper3892]

They can make zombie I guess

[@DevonPhoenix]

Fake

[@tx-sweet-pjg3547]

You know Frankenstein was a monster right ?

[@royaniv]

huh?

[@FahimHoq]

That's fiction

[@patricktibbits5475]

Dr. Frankenstein was the creator of a monster.