Integrating nanoscopy into a biology toolbox: A case for high-throughput imaging and image analysis

Join Rengasayee (Sai) Veeraraghavan, Associate Professor of Biomedical Engineering, discussing Integrating nanoscopy into a multi-scale systems biology toolbox: A case for high-throughput imaging and computational image analysis for our Beyond the Scope: CEMAS Discussion Series.
Description:
Growing evidence indicates that biology and physiology at cellular through organ scales are governed by the action of proteins organized within nanodomains with specialized ultrastructural properties. Multiple phenomena have been identified, whose function and dysfunction cannot be predicted without accounting for the makeup and behavior of nanodomains. Thus, my laboratory’s investigative approach is grounded in high resolution structural and functional imaging, complemented by our development of novel imaging and image analysis approaches. I will present work illustrating how super-resolution microscopy and quantitative image analysis in conjunction with other experimental and computational modeling approaches have enabled us to make inroads into cardiac physiology and develop novel treatments for disease. I will also share recent work on a computationally efficient, hyperparameter-free approach to assess spatial relationships from point coordinates in continuous space, such as yielded by single molecule localization microscopy (SMLM) and how it could enable autonomous, high-throughput nanoscopy.