Knockout mice| NeoR tk gene selection|G418 casette|Techniques in Biology PYQs CSIR NET Part C|Part I

[@pratibhasah6698]

Wow I have never understood this topic so CLR, thanks a lot.such a win lecture

[@yuvarajsubramani2848]

Nice explanation mam.. ...I understood throughly

[@yuvarajsubramani2848]

Thanks lot

[@sirsha9145]

🤩🤩🤩🤩Wows to you😄this was such clear and short video.... Csir is on 17th and I'm watching this for a quick recap. Thank you❤ so much❤ for the nice video 😇

[@02riyabhiwandkar43]

Thanks a lot ma'am. I was unclear on this topic but your video helped me understand everything. 😁🤩

[@IntrovertGeek]

Most welcome 😊

[@sukanya3701]

But TK gene is involved in salvage pathway of nucleotide synthesis.. if in normal gene tk doesn't present how they are going to perform DNA replication?! As after HR, TK will not be inserted into the target gene...

[@sirsha9145]

Hmm.... I'm not sure but just trying to make a point actually this was my question too.... But as host gene itself might have the tk gene therefore host cell might have made tk ... And then there wouldn't be any problem to continue the salvage pathway of nucleotide synthesis 🙂hope you got what I'm trying to say