LIVE Philodendron Subculture with Francisco - Plant Tissue Culture

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[@letsclimb5828]

I’m about to sub culture some plants. Hope it goes well! Appreciate you!!!

[@PlantCellTechnology]

Thanks!

[@linlin-xq4fb]

I'm learning from your video I want to join to your team.

[@faddymac6373]

im curious what are the effects of photoperiodism on the subcultures?

[@tchoradelpuerto353]

I can hear you really well for being under a laminar flow

[@PlantCellTechnology]

That's great!

[@tchoradelpuerto353]

Hello Francisco,,

[@alexiszisakos295]

What PPFD do you usually use for your multiplication? I feel like my LEDs are too weak (~60µmol/m²/s). Also (i don't know if i missed it) what amounts of PGR do you use for multiplication? My Philodendron caramel marble (just the size as those in the video) are on 1mg/l BAP and 0.1mg/l IBA.

[@PlantCellTechnology]

For the lighting, I use a basic 4 ft 6500K 2500 L LED. Tissue culture plants do not need strong light. For the multiplication media, I'm not using IBA anymore. Only BAP.

[@alexiszisakos295]

@@PlantCellTechnology Thank you for your reply. What concentration of BAP do you use?

[@PlantCellTechnology]

For what purpose? @@alexiszisakos295

[@alexiszisakos295]

@@PlantCellTechnology for multiplication just like for the caramel marble in the video :)

[@PlantCellTechnology]

For that purpose we use 3-5 ml/l of BAP@@alexiszisakos295