Universites and professors need to follow Shomu`s way of explanation he is really brilliant. Thank you teacher!
@shomusbiologyofficial2 жыл бұрын
Thank you so much for appreciating my efforts
@Natalia-wd8ye5 ай бұрын
Thank you for this video, it's hard to find info on this specific topic in free access and also you explain it well so it's easy to understand :D
@shomusbiologyofficial5 ай бұрын
You're welcome. Glad to hear that you're getting benefit from my lectures
@samyuktha652 Жыл бұрын
I really need to thank u, ur way of explanation is great. Ur vedios really helped me
@shomusbiologyofficial Жыл бұрын
Thank you so much for appreciating my efforts
@sanjeevakumar49126 жыл бұрын
I request you to make a video on baculovirus vectors. By the way your tutorials are simply amazing
@gayathrig4311 Жыл бұрын
U r an amazing teacher😊
@shomusbiologyofficial Жыл бұрын
Thank you
@sidrah4616 жыл бұрын
Plz do a video on bucculovirus expression vector system in a bsc level
@sumit-lr1qm4 жыл бұрын
দুর্দান্ত স্যার।
@shomusbiologyofficial4 жыл бұрын
Thank you. Glad you liked my lectures
@mayukhroy53934 жыл бұрын
Thank You Sir,for such a clear explanation.
@shomusbiologyofficial4 жыл бұрын
You're welcome
@venkatesanmunusamy73192 жыл бұрын
Thanks
@shomusbiologyofficial2 жыл бұрын
You're welcome
@danielhain404 жыл бұрын
Please make more!!!
@shomusbiologyofficial4 жыл бұрын
Sure
@subhiksharajan85475 жыл бұрын
Please do videos on bpv based vector and insect vector
@KingLewis468 жыл бұрын
Poly-A tag sequence is not for protein isolaton!( 8:16 ) poly A tail is not expressed into protein so you can´t used to fish your protein.
@C0806278 жыл бұрын
Agreed, the hGh Poly A sequence is used because it can improve mRNA stability and boost translation efficiency. Also, to ensure the polyadenylation of the mRNA. In this case, a tag (hexahistidine tag) sequence will allow for protein purification.
@MrRawresh7 жыл бұрын
thank you, was going to say the same thing.
@dubstepXpower5 жыл бұрын
Poly histidine tag right?
@manaspaul87264 жыл бұрын
Sir please make a vedio about T7 promoter based vector
@Garcia-elf8 жыл бұрын
Can you explain what Markov chain Monte Carlo is and how it is applied to genomics?? What does "sample the joint posterior distribution" mean? Thanks
@danielo.39886 жыл бұрын
great videos thank you!!
@shomusbiologyofficial6 жыл бұрын
+Daniel O. Thank you.
@berastainisabel99446 жыл бұрын
Great video, thank you very much for your clarity!! yet, poly A is not a selection tag, be careful!!!
@shomusbiologyofficial6 жыл бұрын
+Berastain Isa thank you. Glad you liked my lectures
@amandeeprana49205 жыл бұрын
God of life science students 😍
@shomusbiologyofficial5 жыл бұрын
Thank you for appreciating my efforts
@amandeeprana49205 жыл бұрын
Shomu's Biology pls make video on phage display sir please
@rhythmdancebymanisha99794 жыл бұрын
What are the different kinds of vectors used in stable and transient transfection in mammalian cells and plant cells????
@ayeshamushtaq93397 жыл бұрын
you great man...
@santoshitekam97522 жыл бұрын
Sir please explain N-terminal p-FLAG-Mac p-FLAG -ATS
@kmrashmi64644 жыл бұрын
Sir plz make video on cassette vector,,,plz soon
@livelovelaugh64055 жыл бұрын
Question- when would u use prokaryotic expression vector and when would u use eukaryotic expression vector? Like in what situations is one better than the other?
@EliasBroeckhoven4 жыл бұрын
The difference is post translational modifications. Prokaryotic organisms are able to produce the same amino acid sequence as the gene would in eukarytic organisms but the prokaryot would not be able to modify the protein (i.e. glycosylation) necessary for the same functioning in both organisms.
@itratzahra23916 жыл бұрын
very nice lectures. kindly suggest any book of gene cloning as easy and brief like ur lectures :)
@amruthamavily6 жыл бұрын
Itrat Zahra life science path finder publications
@Kaoriocha4 жыл бұрын
How do transiently transfected plasmid in the cytosol get expressed? I thought the transcriptional machineries is inside the nucleus...
@ZincAllThumbs5 жыл бұрын
THANK YOU! :)
@shomusbiologyofficial5 жыл бұрын
You're welcome
@janakirawool16614 жыл бұрын
Is there any video on promoter selection with plasmid pKO1? ..I can't understand that promoter selection in pKO1
@SWyrick3663 жыл бұрын
which restriction enzymes are required to clone the mammalian expression vector pMV2 BMPR?
@freefiresaro83 Жыл бұрын
Why we are use 3 ori in mac
@darakhshasaleema1463 жыл бұрын
Why regulatable promoter is used in protein expression vector?
@shomusbiologyofficial3 жыл бұрын
To increase or decrease expression
@ren51244 жыл бұрын
Just a question. So this expression vector will not need to be integrated into a chromosome via homologous recombination for it to be expressed? It can just be introduced into the nucleus of the mammalian cell?
@neuromobmob2 жыл бұрын
I think i love you man xd
@shomusbiologyofficial2 жыл бұрын
Thank you
@anicatasnimprotity1293 жыл бұрын
can a mammalian expression vector be 'transformed' into a bacterial cell?
@tejaswiniingle92944 жыл бұрын
Please do a video on SCP vector
@ratty19424 жыл бұрын
How does the vector become linear, to be inserted into the genome?
@shomusbiologyofficial4 жыл бұрын
If you cut a circular vector at a single point, it will be linear
@imrocknreeling6 жыл бұрын
Why don't it degraded inside the cytosol? Since mammian cell is very prone to detect foreign particles. Please describe
@justicemunyoro4 жыл бұрын
any response?
@motivator69743 жыл бұрын
Plzzz is lecture ky notes dy dyn
@mohammadamir14616 жыл бұрын
can you plz hindi lecturers
@somasaha54853 жыл бұрын
Shomu sir mammalian vector ar mammalian system ki aki plzz reply
@shomusbiologyofficial3 жыл бұрын
No. The word system is vast and vector is a gene delivery system which is specific
@anatolyrozhkov8514 жыл бұрын
Stop, 6:40 why exactly do we need more than one ori?