"Smart Yeast" | Bioengineering PhD Defense

[@DIYBiotech]

Got my PhD :)

[@noelbreitenbach8673]

Congratulations!

[@razyarh4389]

Congratulations 🎉

[@snibbers]

Congratulations! 🥂

[@jamesmihalcik1310]

Congratulations, well deserved. ✨

[@ironyelegy]

GRATZ 🎉

[@jedidiahlingenfelter7925]

Congratulations Doctor! I’ve been subscribed to the channel for a little bit now, and really enjoy watching your videos. I just got accepted into the Plant Pathology PhD program at UCR and I’m excited to start!

[@DIYBiotech]

Wow that's awesome! Welcome to Riverside! Send me a message on Insta if you have any questions about getting started. I know a lot of people in plant bio here.

[@MMbyAlexander]

Congratulations!!! Can't believe i stumbled across your video and here you are getting your PhD. Bravo!

[@DIYBiotech]

Thank you so much!

[@Sugar3Glider]

The ability to select for terps has me quite excited. There's a surprisingly high number of benefits from them specifically, and they seem to be selective in that different terps have different effects.

[@SixOhFive]

Genome wide CRISPR screens seem like a powerful tool.

[@DIYBiotech]

They're so cool, Chad Giga!

[@itskarl79]

@@DIYBiotech a lot of room for unethical misuse too, especially exposure/implementation without consumer awareness; the potential misuses violates inherent medical autonomy. I hope you have a conscience and forsight to the ways people/government/corporations might use your work/efforts. In a sense it is genomic assault, if areas of the body are affected without permission/against our will. We saw how vocid triggered DNA changes in organs through CRISPR/mnra tech after people across the planet were forced to receive "treatment".

[@the_grunkler]

As someone wanting to pursue biology research and someone who has recently found and now love and almost depend on the benefits of THC and psilocybin, I've become increasingly worried about being perceived as a drug addict and also becoming one, but seeing you succeed and finishing something this big while also releasing public videos with interest in these compounds gives me hope

[@gabrielepirovano3529]

I’m a MENSA member with a PhD Before smoking THC daily I wasn’t even enrolled in uni Am I an addict? Maybe, but ever since that day I am an academic weapon as well Don’t let other people’s perception of you change your life But be careful with shrooms, they are good once every often to ground and guide you, too many and you may lose your focus and purpose in life Same goes for LSD, I’ve lost a couple of years on a side quest They weren’t wasted, but for sure did delay my life quest for a bit as I was learning more spiritual stuff

[@bradarmstrong1656]

Great channel! I hope you are a PhD. now!

[@DIYBiotech]

There's less and less stigma around these types of projects! More and more old heads with more conservative views are retiring by the day!

[@SunriseLAW]

"found and now love", "almost depend on the benefits of THC and psilocybin" and "drug addict" are various labels for the same basic concept. I watched several DIY Biotech vids (he made a bunch a couple years ago). I do not remember noticing any indication the video creator enjoys consuming THC or psilocybin as much as you do. He might, but I doubt it.

[@devkkev9066]

Awesome, u are awesome. Keep up the great work! Love your tendency towards details in your info-vids, just great to see someone so interested in showing things they love and know about.. Thanks!

[@DIYBiotech]

Glad you like them! Thanks for the support!

[@MrBarryyoung]

Congratulations, Dr. Robertson! 👏 Fascinating studies. I hope to one day follow in your footsteps. Could you please go into more detail about how you modulate your cells' metabolism for growth and production phases? Good luck in all your future endeavors!

[@DIYBiotech]

I'll review that paper soon! It's a really cool one. Thanks for the congrats and good luck with your PhD!

[@101ziner]

Well done!!

[@DIYBiotech]

Thank you!!

[@That_Chemist]

Is there a good strain of yeast which has been engineered to break down waste carbohydrates without generating undesirable volatiles? Or could you setup an air pump into a bioreactor and use a carbon filter to scrub the smell?

[@anyhuman100]

Congrats man!! Good material, I’ve been a software/data engineer for quite some time and can definitely see how this is very similar to coding/engineering and is super rewarding/liberating

[@DIYBiotech]

Thank you!!

[@nicholassayegh9341]

Very interesting and definitely gets me excited about the future of smart yeasts!!! Thank you so much for sharing your work!

[@DIYBiotech]

Glad you enjoyed it!

[@nicholassayegh9341]

@@DIYBiotechI’m in my last year as a mechanical engineering student at CBU (down the street from you). I’ve thought about graduate school for a while now and your excitement and enthusiasm is definitely making an influence. I’m interested to see more about what you do so thank you for sharing it with everyone!!!

[@DIYBiotech]

@@nicholassayegh9341 very cool! Theres a new PhD in my lab that came from CBU!

[@SunriseLAW]

I worry about the future of smart yeast infections.

[@dabert5972]

CONGRATULATIONS MY MAN!!! Thank you so much for your videos!! As a software engineer, I've started hearing about how badly people like me are needed in fields like bioinformatics and I used to REALLY like science back in high school, so I've been thinking about going something more in this direction. I am completely self-taught and dropped out of school my sophomore year, but I've been thinking about going back (for NOT a comp sci degree lol) and I'm curious what kind of degrees you would suggest I look into if I want to work with yeast!! Seeing the broad spectrum of naturally occurring psychoactive substances makes me wonder what other kind of natural compounds can be manufactured with yeast

[@DIYBiotech]

There's a HUGE need for coding types in biotech right now. I'm sure you could slide into a biotech position with just comp sci background. If you're looking for a master's or PhD in genetic engineering type things, look into biochemical engineering, bioengineering, or biological engineering programs. Definitely look to see what professors are researching before applying. My lab is chemical engineering, but we only do biology.

[@brandonprescott5525]

Great watch, good luck, and (assuredly) congratulations!

[@DIYBiotech]

Thank you kindly!

[@zacharytack6932]

Extraordinary video! One question: you’re scanning ALL potential [gene knockout locations? CRISPR guides?], for optimal gene placement location/efficiency, then manually evolving them to further suit the task by making the yeast report production r8 for you??? you’re essentially just plugging the output activation from the biosensor (programmatically) into growth factors or other genes? This is really cool!

[@zacharytack6932]

i take it you could eventually configure multiple biosensors/genes as a “program” (reproduce until quorum sense; produce and export [molecule] when at concentration)? would using smart yeast in practice be similar to regular alcoholic fermentation (plus purification)?

[@DIYBiotech]

Yes exactly!

[@miketout]

Awesome work! Thanks for sharing!

[@DIYBiotech]

Thank you! Cheers!

[@joeypaisano9235]

Listen u got the shroom starter yeast or not?

[@aaronderoo4327]

Right?

[@gabrielepirovano3529]

Man’s pizza gotta be magical

[@SixOhFive]

1 mil per ml

[@selfisher1020]

The people want yeast!

[@codemiesterbeats]

😂🤣 funnier than it should be

[@Leonardo-u4u2f]

This is so cool!!!

[@marcomoriarty7692]

can you give us some tips on how to get started with genetic engineering at home?

[@srikar434]

Congratulations you're an inspiration 🎉

[@DIYBiotech]

Thanks :)

[@philipemmanuele8935]

Some questions about the last chapter. It seems like one of the knockouts produces more terpenes simply because it can grow faster whereas the other one optimized some terpene synthesis pathway. Did you make a strain with both knockouts? Also, did you use barcodes when making your library or did you sequence the entire genome of your increased fitness mutants to find the KO. Congrats on passing and great video!

[@DIYBiotech]

Great questions! The two best knockouts grow the same on regular media, they only grow different when there are high concentration of terpene added to the media. Both knockouts would be a good next step! Yes, each sample of our genome-wide screens are barcoded so we know which flask the guide counts came from. We only have to sequence the gRNA on the plasmid, not the whole genome. Another reason why CRISPR screens are great!

[@ZelosDomingo]

Great video, you really embody that whole "if you can't explain something simply, you don't actually understand it." quote. Feynman, I think? But you clearly know your stuff. 😺 I was really curious though, near the end when you're talking about the two knockout strains vs the base, did you create a strain that had BOTH of those genes knocked out? Really curious to see whether production would go up more or whether they would interfere with each other. Or is that like, hard to do for some reason?

[@DIYBiotech]

Great question. One of the professors on my committee asked the same thing! No I didn't try that, but yes, there is a good chance it would improve titers even more. But, these genes also serve other functions, so the more knockouts you make, the more likely the overall health of the cell decreases

[@darkoverlod8171]

this shit is sooooo interesting, im currently a biology undergrad in hohenheim germany and fungi and genom editing got me into it. I hope to become a metabolic engineer as well:)

[@DIYBiotech]

Do it! Such a fascinating field!

[@arioche]

Hey there! Congratulations on your PhD! I'm curious about how much our thinking aligns. I'm a DIY enthusiast and a microbiologist, and I would love some guidance on pursuing a PhD. Would it be okay if I emailed you with some questions?

[@DIYBiotech]

Thank you! Absolutely shoot me an email! [e-mail]

[@merbst]

Yay for UCR! I ❤ Riverside!

[@nanaperrucci3775]

Congrats🎉 very cool! As you are an expert in yeast cultivation, I would like to ask you which method to use for protein determination in supernatants? Tried Bradford but got huge differences in protein content comparing to kjeldahl... don't know why that happens...what did you use? Would be great to get your opinion on that 😊

[@DIYBiotech]

I did BCA for some algal protein quantification a while back! I've got a video of it on my channel

[@jonahbranch5625]

Good luck bro. Great explanation too :)

[@DIYBiotech]

Appreciate it!

[@LevmurQ_Q]

Good luck to you!

[@a.v.gavrilov]

Can you do work by adding "feeding energy by electricity" to Yeast from electric-powered (electric feeding) bacterias? Its seems can be interesting for industry "precision fermentation microbiological protein production" for example, for all Yeast-based industry

[@DIYBiotech]

Two things here: 1. you can make carbon sources with electricity and CO2 (artificial photosynthesis) 2. you can provide energy to cells in the form of electricity But energy and carbon source are two separate things, just to be clear

[@Sugar3Glider]

Do you capture the gas released by the yeast and study its composition to see what the yeast is in excess of or lacking in their environment? Kinda like measuring the chemical expressed when your gut bacteria die or whatever to send chemical signals to you.

[@DIYBiotech]

I'm sure you could! But I don't know of much research in this area.

[@just_bright]

I've seen two cures for cancer grown in yeast in my almost 40 years. Both were meant to be huge successful new medical products and both will never see the use for our children in America. It's been so long since I got jaded, I almost forgot....

[@a.v.gavrilov]

I know that Krasnoyarsk Institute of Biophysics lab "Bioluminescence of sea" work for Bayer - they searched genes of bioluminescence for Bayer's did sensors like you do for automatic cellar drags experiments (100 000 tests per a day line, thay said). How it can be helpful for your work?

[@nesiansides7133]

Biosensors cool technique 😎

[@DIYBiotech]

Biosensor video coming out today!

[@ganonrosario6627]

Hello, congrats! Your research is very interesting. I am also doing a phd in Biotech and I wanted to ask: Do you got training during the first few weeks of a laboratory class? I take laboratory classes where the professors don't train us whatsoever and delegate all the work on us. To me it seems they're just lazy. Is that the way it is in phd programs or not?

[@DIYBiotech]

Keep it up! Yes, in my experience, PhD is a whole lot of independent learning

[@fnytnqsladcgqlefzcqxlzlcgj9220]

Good shit!!

[@Walt-z4u]

good luck!

[@TheD3cline]

hell ya bro

[@TRex-ws3vp]

You rock

[@DIYBiotech]

No u :)

[@meg.h.]

wholesome

[@DIYBiotech]

:)

[@just_bright]

Hey Nick! Been following ya for a bit but didn't know we are neighbors! Let's grab a beer man! I'd love to rap out about this stuff! Hit me back if your free and congrats on getting your PhD! By the way I'm also published twice so far in random medical stuff we did at our medical center in Tustin. Cheers!

[@DIYBiotech]

Shoot me an email or DM on instagram!

[@SunriseLAW]

KZbin "clicks" on the horrible... what can go wrong, how the technology could be used to create super-bugs of all types. What about yeast that produces psilocybin and then women get yeast infections that cause them to endlessly trip. My guess? The video creator is already employed or in the process of evaluating various highest-paying offers.

[@DIYBiotech]

Don't worry bud I won't sell out and work for pharma or defense contractors! Unfortunately a lot of my colleagues do...

[@420algorithm6]

Is it alright if I call you my fliend?

[@DIYBiotech]

Ah! My fliend!

[@citricdemon]

"smart yeast" is what i call my girlfriend when she forgets to shower again.

[@merbst]

smart yeast is good for laughing at the highbrow jokes.

[@TrevoltIV]

"Obese Yeast"

[@petevenuti7355]

Make sure you characterize all the false positives! Especially if any of this is going to be used for drug testing.

[@Chad_Elliott]

bro nothings gonna happen if you read his unpublished stuff if anything itll promote the book itself and youll teach people. I just dislike when people hide information that could potentially be used for good, like the feds and weed, and how theyve beeb growing it the whole time since 1978