Mapping and aligning sequencing reads | NGS read preprocessing in R (Part 3)

This is part 3 of the NGS read processing tutorial, where we will go over the basics steps of preprocessing for next-generation sequencing reads in R. We will use the packages QuasR and Rqc. With this step-by-step guide you can follow the standard pipeline.
We will go through the basic steps of quality control and filtering, trimming reads, and finally mapping them onto a reference genome.
Hope you like it!
Link to dataset:
bioconductor.org/packages/rel...
Step by step explanation:
biostatsquid.com/sequencing-r...
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Don’t forget to subscribe if you don’t want to miss another video from me! -------------------------------------------------------------------------------------------------------------------- Other interesting resources for sequencing reads mapping:
ieeexplore.ieee.org/document/...
More on the Bowtie alignment algorithm:
www.ncbi.nlm.nih.gov/pmc/arti...

[@mihacerne7313]

What an extensive tutorial! Wohoooo

[@biostatsquid]

Here is the final part 3 of our step-by-step tutorial on sequencing reads preprocessing and alignment! Forgot to mention it in the video, but you can see how the preprocessed reads show better mapping results than the raw fastq files - that's why quality control is so important! Let me know how you like it and if you would like more tutorials like this one:)