Mechanism of RNA Editing | Site Specific Deamination

Previous video : Overview of RNA Editing
The editing involves cytidine deaminase that deaminates a cytidine base into a uridine base. An example of C-to-U editing is with the apolipoprotein B gene in humans. Apo B100 is expressed in the liver and apo B48 is expressed in the intestines. In the intestines, the mRNA has a CAA sequence edited to be UAA, a stop codon, thus producing the shorter B48 form. C-to-U editing often occurs in the mitochondrial RNA of flowering plants. Different plants have different degrees of C-to-U editing.
Adenosine-to-inosine (A-to-I) modifications contribute to nearly 90% of all editing events in RNA. The deamination of adenosine is catalyzed by the double-stranded RNA-specific adenosine deaminase (ADAR), which typically acts on pre-mRNAs. The deamination of adenosine to inosine disrupts and destabilizes the dsRNA base pairing, therefore rendering that particular dsRNA less able to produce siRNA, which interferes with the RNAi pathway.
RNA editing is a molecular process through which some cells can make discrete changes to specific nucleotide sequences within an RNA molecule after it has been generated by RNA polymerase. It occurs in all living organisms and is one of the most evolutionarily conserved properties of RNAs. RNA editing may include the insertion, deletion, and base substitution of nucleotides within the RNA molecule. RNA editing is relatively rare, with common forms of RNA processing (e.g. splicing, 5'-capping, and 3'-polyadenylation) not usually considered as editing. It can affect the activity, localization as well as stability of RNAs, and has been linked with human diseases.

[@Merin-io8lp]

The description box has the best notes for rna editing. wonderful explanation.

[@hussainbiology]

thanks for appreciation.. Glad it helps ✌️

[@dmbms]

Thank you

[@priyankaswami6552]

Am the first to comment here..🥰🥰

[@manal5148]

Sir can you please make a video on Holliday Junction and DNA Damage and Repair. Thank you for this amazing video.

[@michaeleisenberg7867]

Shabir, At first glance RNA editing might seem like a good idea to save space on the DNA and still get more proteins. But it took a lot of DNA just to code for all the editing proteins. We are never going to fully understand this 🙁🔬🧬...

[@jldantzler]

Thank you for the video, Hussain! @ 2:26, 'CAA' does code for glutamine, but the 1-letter symbol for this amino acid is "Q". "G" represents glycine.

[@Lazizbek_Akhmedov]

I really liked it.What program do you use to edit?

[@hussainbiology]

Final Cut Pro

[@neetbiologybyrashidbashir6498]

You never disappoint...!

[@divyaprajapati4337]

Nice video And nice explanation sir🙏

[@hussainbiology]

thanks for appreciation..Glad it helps....✌️

[@ageeshnaayyappan8616]

Please do a video for tetrapod limb development.

[@divyaprajapati4337]

Sir mutation ke bhi bna sakte ho ap

[@hussainbiology]

Haan Pakka i will

[@MissBoundlessMusic]

4:22

[@hussainbiology]

so what is at this point... ,( i say remember)) or i have made a mistake??? is it

[@MissBoundlessMusic]

I had little confusion at this point sir but thankfully you cleared my doubt.

[@varshapoly2138]

Thank you for the video. Can you please upload a video on RNA editing in Glutamate Receptor Subunit.

[@hebaomar3001]

Amazing explanation

[@hussainbiology]

thanks Heba for appreciation..Glad it helps ✌️

[@roadmates1]

Wow your voice

[@hussainbiology]

thanks

[@sumayashah8564]

Variya asel

[@hussainbiology]

Shukriya..... Gov samgh aav Kashur chus

[@hussainbiology]

@@sumayashah8564 that is amazing.... Really glad to know that ✌️

[@malikishfaq2978]

shukriyah reveal karne k liye that yi chhu kaeshur 👍

[@aaqibhameed2972]

👍

[@craftclicks]

RecBCD model .... any video ?

[@michaeleisenberg7867]

Good Morning Shabir! That was brilliant. Your animations were spectacular. It is incredible how these pathways evolved and maybe even more incredible how the scientists figured it out. You must have an x-ray crystallography machine on your desk. 🎯🎈👏🎡🎢🧬🩻🎁🎂🔬 Thank you very much. 🐕🐕

[@hussainbiology]

Good Morning Dr. Mike.... first of all thanks for appreciation... and yes these Pathways are amazing ❤️❤️