MEGA X: Reconstructing Ancestral Sequences

[@Nicole-rq2bq]

This video, and the extra script to organise the ancestral output, were fantastic. Thanks so much for making this!

[@leonhardschnittger9743]

Thanks a lot for this great explanation of ancestral sequences...!

[@matteo311290]

At min 17:30: On Windows 10, after I navigated to the folder, I had to enter: ExtAncMEGA.exe ebgCanc.txt Otherwise, I was getting an error log without the ".exe". PS: thanks for the video :)

[@annabellelevin4425]

How do you run the program using amino acid sequences?

[@a1batr0ss23]

Hello, maybe a little late but I would like to know if the ExtAncSeqMEGA is still available. I cannot find it on the given website. :(

[@anusharai3207]

Can we still use MEGAX for generation because recently when I tried using MEGAX there was a popup which said not to use the trees generated for the publication. In MEGA7 how do we do for the G+I parameters?

[@AqleemAbbas]

Great video, Can we use the same protocol for ITS sequences and 16S sequences from the company that has sent us?

[@shammabooth7370]

Hello, unfortunately after I work my way through the pipeline I seem to get no ancestral protein. I always get the same "Ancestral Protein Sequence: The DNA sequence is not a coding sequence ( the length is not a multiple of 3). No ancestral protein is calculated." Do you have any advice as to why this is the case? Thank you!

[@lukemyers5494]

Maybe a little late, but if you are using protein reconstruction, you need to trim your DNA sequence to have a number of bases divisible by 3. Three nucleotide bases make an amino acid.