Microbiome/Metagenome Analysis Workshop: DADA2

[@najmujsakib7088]

Crystal clear! Thank you.

[@eviehymas5520]

Such a helpful lecture with wonderfully clear explanations, thank you!

[@aquibkhan9997]

At 41:09, where you are creating variable named "map", you have used a file "Tutorial_Map.txt"... Can you tell or provide any link from where we can get this file ?

[@leandrodigloria526]

Your presentation is extremely clear. Thank you so much!

[@adityabarde476]

These are really good explanatory videos from the workshop. I would like to see the other videos.

[@TheMookieism]

great introduction to DADA2. thanks for that

[@miguelmmb3]

so good! It helped me a lot

[@bedoelHabib]

can you put the code used in this tutorial in a comment or in the description?

[@hanneloorheynderickx7663]

Thank you for this! Your video is great.

[@mansisharma5429]

I am running in a problem with filter and trim command. I am not getting any results after running this command. It says check your parameters for filtering.no gz file written. Kindly help me out for this issue

[@naturelab-80]

Why I couldn't run error rate estimation function? The error was coming out as below: > errF

[@Zabuzakashi]

R can't find the function - it could be you need to reload your library(dada2) line, also double-check that you didn't misspell filtFs (or whatever your named list of files is). Are you using a mac or a windows?

[@곰팅구리]

I'm having trouble installing the 'dada2' package in the latest version of R. Could you suggest a compatible version of the R package?

[@endrewsantos8831]

Did you solve it?

[@ranjanmanishblue]

if i got Identified 118496 bimeras out of 125600 input sequences. during running command seqtab.nochim

[@sparklessr5484]

Amazing video! Is the Phyloseq video available?

[@marcelozerillo3551]

dereplication is not part of DADA2, is it? What did you use?

[@soyeonkim9355]

good explanation! thanks alot!

[@weilin2910]

where do we find the Tutorial_Map file?

[@aquibkhan9997]

Did you find this file... I'm also unable to get it

[@AqleemAbbas]

Why always quality of reverse reads decreases more than forward read

[@sotiriosvasileiadis7260]

maybe mostly due to intensification of over-clustering (clusters grow in size due to the extra bridge amplification reaction performed for reversing the DNA fragments and approximate each other, thus, lowering the purity of sequencing signals of neighboring fragments).