Determination of Acid value & Free Fatty Acid(FFA)_A Complete Procedure (AOAC 940.28 & ISO 660.2009)

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MicroChem's Experiments

MicroChem's Experiments

Күн бұрын

Пікірлер: 398
@chemistbd886
@chemistbd886 3 жыл бұрын
Thanks a lot such types of video uploaded. I see and saw your all video and i feel very happiness. Great wishes for you and your channel.
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Thanks.
@bellabconcept1432
@bellabconcept1432 Жыл бұрын
Thank you for complete step and most easiest method you are using. I never regret of knowing this great Institute of Learning. Nevertheless, free fatty Acids determination was not done in this video
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Thanks. Free Fatty Acid calculation is given in the description section. Please take a look
@HashmiMariam
@HashmiMariam 2 ай бұрын
Thanks My student is writing an IA n this it helped her to set the methodology
@MicroChemsExperiments
@MicroChemsExperiments 2 ай бұрын
Thanks for sharing this
@emanroshanara3799
@emanroshanara3799 Жыл бұрын
Tomorrow is my practical and it is helping me alot From Pakistan
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Glad to know
@karishmasaini4076
@karishmasaini4076 25 күн бұрын
Same to you
@IrfanAli-xy5bf
@IrfanAli-xy5bf 4 жыл бұрын
Miss thanks for sharing, yours methods are so easy which helps easy understanding. Keep it up.
@MicroChemsExperiments
@MicroChemsExperiments 4 жыл бұрын
My pleasure 😊
@frankcastle4657
@frankcastle4657 3 жыл бұрын
MicroChem's Experiments people, I just saw your posts today and you are such smart, human angels for sharing your knowledge in such a simple and easy way to understand. Thank you so much! May God/Karma/Life blesses you and your loved ones today and always! Do you have any videos of how to prepare samples, standards, control, and blanks for ICP-OES? And how to do analysis with a GC and an UV? Thank you, you are appreciated!
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Thank you very much for your appreciations. We do not have video for ICP-OES. In future, we will try to make video on it. We will upload video on GC soon. Stay with us.
@sachinap7141
@sachinap7141 2 жыл бұрын
I am microbiology test only analysis,but your video will help to learn Crude protein analysis,crude fiber and crude fat test is very easy and result is very good
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Thank you soo much for such appreciation. We uploaded standard preparation for AAS. We. Also uploaded one video for GC and several video for spectrophotometer analysis. In future we will upload for ICP-MS too. Stay with us. Soryy for late reply.
@abigailabu9299
@abigailabu9299 9 ай бұрын
Very educating and simple too, but please how do I estimate the free fatty acid from the acid value dealing with Shea butter sample
@ujjwalkoirala6770
@ujjwalkoirala6770 9 ай бұрын
Can we use 0.5%phenopthalein indicator??For av determination.
@muazashraf3416
@muazashraf3416 25 күн бұрын
I did this today. If ethanol is not contaminated then it is already neutral. So you can skip the naturalization of ethanol. In today's practical I started titrateing ethanol. At 1st drop of NaOH, the ethanol turn dark pink (due to indicator) because it was already neutral.
@buonyduopkuek
@buonyduopkuek 6 ай бұрын
Thank you for your upload videos and I'm very interested to learn that teaching for easily understanding!!!!❤❤❤❤ I'm new for your channel and I will introduce my position to you!!! I'm one among chemistry student: 1. Under graduate, 2. Bachelor degree, 3. 4th year Thank you so much and I will follow you always !!!!!
@vidhyav8650
@vidhyav8650 Жыл бұрын
hello ..i have a doubt ....to get FFA in percentage wew have to use the same formula and also FFA does not have any unit....another question can i use formula for the estimation of FFA in Pearl millet flour..? kindly give me a suggestion...
@MicroChemsExperiments
@MicroChemsExperiments 4 жыл бұрын
NOTE: There is a mistake in the formulae of acid value, mentioned in this video, and the formulae for FFA is not given in this video. Both corrected formulae & calculations of Acid value and FFA are given below: *Acid value (as oleic acid) = 56.1×V×N --------------- W So, Acid Value (mg/g) = 1.6 *Free fatty acids (as oleic acid)= 28.2×V×N --------------- W So, FFA = 0.805 percent by weight
@AriffMahtar
@AriffMahtar 3 жыл бұрын
i have noticed in the early video, thank you for correction!
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
@@AriffMahtar Actually we did a silly mistake in the calculation. Please ingore it.
@crayversace2804
@crayversace2804 3 жыл бұрын
Isn't 56.1 the molecular weight for KOH.. And you used NaOH in the experiment
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
@@crayversace2804 AOAC method said to use 56.1 as the factor of this calculation for in both of the cases whether you use 0.1N KOH or 0.1N NaOH solution.
@crayversace2804
@crayversace2804 3 жыл бұрын
So what would the formula be if I used 0.1 KOH, 10 grams of oil, and had a titration volume of 0.76
@Lila-lc7wd
@Lila-lc7wd 11 ай бұрын
Hi, thanks for the video. Why do we boil the ethanol-sample solution and what percent phenolphthalein solution are you using? Also, can I neutralize ethanol using KOH instead of NaOH? Thanks again!
@عبداللهمهيوب-ر3غ
@عبداللهمهيوب-ر3غ 2 жыл бұрын
When I needed to get Acid value in our laboratory for fats I used 0.1 N NaOH for titration , but when I calculated I multiple by 56.1 . our specification in our laboratory said that. Is that right?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Right
@عبداللهمهيوب-ر3غ
@عبداللهمهيوب-ر3غ Жыл бұрын
But why ? Please give me the reason I think when you titrated with 0.1 NaOH you multiple by 40.001. so I said to my boss that our specification is wrong because we multiple by 56.1
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
@@عبداللهمهيوب-ر3غ if you follow AOAC method, it is written that you have to use factor 56.1 whether you use KOH or NaOH
@tasbeehghareeb5931
@tasbeehghareeb5931 6 ай бұрын
Thanks a lot for your effort. What is the range of acid value?
@ANPakistani
@ANPakistani 2 жыл бұрын
Thanks for sharing easy way to learn. Why you boiled the sample
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
For proper mixing of oil
@grebulocities8225
@grebulocities8225 2 жыл бұрын
Fats/oils don't dissolve fully in ethanol at room temperature but do dissolve at the boiling point of ethanol. For this test to be accurate, the oil needs to be fully dissolved in the ethanol, so the ethanol is heated to its boiling point to ensure the oil is all dissolved.
@anjumanara6967
@anjumanara6967 Жыл бұрын
Can I use this method to know bacterial acid value? And my lipid content of bacteria is. 0085gm then the procedure will be the same? Like 50 ml ethanol etc
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
No idea about your sample. You can try and let me know the results.
@mysticforest6345
@mysticforest6345 3 жыл бұрын
Hello, I have some questions. What is the difference between normality and molarity? What is the purpose of standardizing the solution? Finally, why do we need to do the first titration of the ethanol?
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
1. You can find the difference between normality and molary from google. 2. Purpose of stadardization is to know the exact concentration of any solution. 3. Normally ethanol is slightly acidic. So first titration of ethanol is done to make the ethanol neutral as if it does not interfare the acidity of our oil sample.
@mysticforest6345
@mysticforest6345 3 жыл бұрын
@@MicroChemsExperiments So for every trial of this procedure, do I need to standardize the solution every time?
@mysticforest6345
@mysticforest6345 3 жыл бұрын
Also, may I ask where you got the expected acid values from? I need a citation. Can you please also explain the formula in the calculation section? Thank you very much for your help. I appreciate it :)
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
@@mysticforest6345 No. But standardize when you will analyze your sample on a different day.
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
@@mysticforest6345 Search on Google and find the reference value from journals and publications. You can also find it from countrys standard of that sample.
@vidhyav8650
@vidhyav8650 Жыл бұрын
my keen request to post a video on lipase estimation by titration method for flour samples .....
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Will try
@harishankarpatel8716
@harishankarpatel8716 2 жыл бұрын
Thanku so much Mam Acid value of oil experiment ke liye🙏🙏🙏🙏🙏
@faijtrds
@faijtrds 3 жыл бұрын
Thanks so much Maam For giv6 In detail knowledge for us
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
You are most welcome.
@MrLongKR
@MrLongKR 9 ай бұрын
Hi. Where can we get the official copy of this method? is it available online?
@MicroChemsExperiments
@MicroChemsExperiments 9 ай бұрын
Not available at online. You can purchase from us. Write us at mail.mic.chem@gmail.com to purchase
@manzano_ja
@manzano_ja 7 ай бұрын
Hi, do you know if I could use Isopropanol instead of Ethanol?
@AshishKumar-rf5tv
@AshishKumar-rf5tv 2 жыл бұрын
What was the actual temperature of the Hot Stirrer during heating the sample with the neutralizing Ethanol? Thanks for your prompt feedback.
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
You need to heat just at the boiling point of the ethanol. May be at 80 degree Celsius.
@AshishKumar-rf5tv
@AshishKumar-rf5tv 2 жыл бұрын
@@MicroChemsExperiments thank you very much!!!
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
@@AshishKumar-rf5tv you are welcome Sir
@tee4052
@tee4052 Жыл бұрын
I did acid value today on hydrogenated vegetable oil as per USP method. United States pharmacopeia. They say to melt the sample (was in powder form) and add 100 ml of hot alcohol previously neutralized with NaOH, to phenolphthalein ts. Then add 1 ml of the indicator. How would you interpret this? Do I add the naoh to the alcohol first and then heat or do I add after heating? And the indicator after heating? Pls help. I think I messed up here somewhere so it didn't pass
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Do as done in this video
@sonofasnitch2172
@sonofasnitch2172 4 жыл бұрын
i wanna ask, we do the titration while the mixture is still hot or not?
@MicroChemsExperiments
@MicroChemsExperiments 4 жыл бұрын
Son of snitch, do titration while it is hot.
@hastesidagro-allied1676
@hastesidagro-allied1676 4 ай бұрын
What is the unit lf the final value gotten. Is it in percentage or mgKOH/g
@putrikaurong9292
@putrikaurong9292 Жыл бұрын
Is it okay if im using methanol instead of ethanol?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
No. That will be wrong
@arslanmunir9334
@arslanmunir9334 Жыл бұрын
Can you please provide a documented file or article of the procedure ?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Sorry. We don't provide any document.
@lev02v
@lev02v 5 ай бұрын
How to avoid measuring mineral acids (e.g. sulfuric acid) and organic acids, only I would like analyze Free fatty acid content.??
@SwhyBe
@SwhyBe 2 жыл бұрын
Great video still have question when I want to measure the acid value of candelilla wax it starts to solidified in the middle of titration ? How to solve this ? And will it affect 9n acid value?
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Keep the flask on hot plate while titration and use magnetic stirrer to agitate the solution.
@vishnukanthful
@vishnukanthful 3 жыл бұрын
Whats the procedure for groundnut oil ?
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Same procedure will be applicable for groundnut oil sample.
@atiqsaifi9615
@atiqsaifi9615 Жыл бұрын
Thanks from your good video. I have a question. Can we use acetic acid 33٪ instead of concentrated by adding three times more acetic acid ؟
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
You do not need acetic acid in this experiment
@azaruameer3931
@azaruameer3931 2 жыл бұрын
Can you give me the "Determination of Oil Analysis" manual if I need it?
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
No sir. No documents will be provided
@virengemini6392
@virengemini6392 Жыл бұрын
When the sample is prepared you are boiling it, but my experiment wants to see the effect of frying on acid value so is it possible to do it in a different way to prevent boiling of the sample?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
No. Boiling is needed for melting, dissolving and mixing of oil with ethanol. Boiling does not change the acid value significantly
@shyamgl9
@shyamgl9 Жыл бұрын
Sir i have a question . Is there any machine available to check moisture for ghee?like the BR check machine .
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
You can use general moisture analyzer
@oluwaseyiomotayo785
@oluwaseyiomotayo785 3 жыл бұрын
Thanks for the video. A question though,what about orange peel oil!? What procedure is useful?
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
This method is applicable for all kinds of oil sample.
@oluwaseyiomotayo785
@oluwaseyiomotayo785 3 жыл бұрын
@@MicroChemsExperiments thank you. About the standardization, how is it performed please? The steps weren't mentioned here.
@Cookie-wt4uz
@Cookie-wt4uz 2 жыл бұрын
What is the difference between ffa value and acid value? And also mass of oleic acid is 282.5 so why 28.2?
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Take help from Google for detailed explanation
@Rahulpatle-14
@Rahulpatle-14 4 жыл бұрын
very informative video...
@MicroChemsExperiments
@MicroChemsExperiments 4 жыл бұрын
Rahul Pawara, thank you so much. Be with us.
@aicha98-ii7xc
@aicha98-ii7xc Жыл бұрын
Please Ihave a question, why did you heat the ethanol with sample and what is the time required to heat
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Boiling is needed for melting, dissolving and mixing of oil with ethanol. Boil for 1 min
@dhruvkadivar2312
@dhruvkadivar2312 2 жыл бұрын
Hello sir/mam, First of thank you so much your videos is valuable for foods company’s. I have two help from your side so 1st questions > how to determination of oil% & protein in Groundnut oil cake ? 2nd questions > how to determination of oil % in Groundnut seed (Peanut) ? Please sir/mam i hope you are making this two questions videos share on youtube…
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Please follow our video for the Determination of crude protein and crude fat. Search on our channel to find those videos. I think you will find them easily
@shubhamrawat8028
@shubhamrawat8028 Жыл бұрын
UTU Chemistry lab teacher........🚀🚀
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Appreciate 👍
@TijanaVasiljevic-nd8fx
@TijanaVasiljevic-nd8fx Жыл бұрын
This is great! Thank you :D
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Glad you like it!
@dayooshilaru9313
@dayooshilaru9313 2 жыл бұрын
Thank you for this video. I want to ask, if we use KOH as the titrant, definitely the formula will will take molecular weight of KOH?
@grebulocities8225
@grebulocities8225 2 жыл бұрын
Some measures of free fatty acids use KOH as the standard while others use NaOH. To convert from KOH to NaOH, multiply by 40/56.11. Likewise, multiply by 56.11/40 for the opposite conversion. This ratio is just the molecular weight of NaOH to KOH.
@grebulocities8225
@grebulocities8225 2 жыл бұрын
To be clear, the AOAC method uses KOH. If you're titrating with KOH, just use the KOH MW (56.11) instead of 40, and you'll have the right acid value according to the AOAC method.
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Yes, absolutely
@marchiter8811
@marchiter8811 3 жыл бұрын
Why do we put an equal amount of alcohol and ether when determining or performing for acid value?
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
We use 50ml of neutralized ethanol to dissolve the oil sample because AOAC method said so
@sarakhalid6469
@sarakhalid6469 3 жыл бұрын
Thanks for those videos❤️‍🔥❤️‍🔥
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
You are welcome
@juliusau1860
@juliusau1860 2 жыл бұрын
What does blank value and titration value in the calculator mean ?
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Your question is not clear
@grebulocities8225
@grebulocities8225 2 жыл бұрын
I'm assuming your method assumes you don't titrate your ethanol before you add the oil. If so, then you'll titrate the ethanol without oil and use this titre (mL after titration - mL before titration) as your blank. Then titrate the ethanol with the oil fully dissolved, and this titre will be your titration value.
@tracywong3311
@tracywong3311 2 жыл бұрын
May I know how long did the oil sample+ neutralized alcohol took to boil?
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
It depends on the temperature you applied. Time doesn't matter, you just need to boil. That's all.
@medamine5787
@medamine5787 Жыл бұрын
thank you for this clarifying experiment, i have a question, you used 10 g of oil, bcs i don't have enough samples what if i used only 2 g of oil, do i need to divide all mixtures by 5? Please reply me
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
g is not enough for the analysis. Do the experiment using 10g.
@Foodtechie000
@Foodtechie000 4 ай бұрын
How to find out free fatty acid value in cake mix?
@RathiVijnana
@RathiVijnana 8 ай бұрын
= 28.2* T.V * Normality NaOH/Weight of sample
@MicroChemsExperiments
@MicroChemsExperiments 8 ай бұрын
Yes. Correct
@muhammadaltaf9651
@muhammadaltaf9651 3 жыл бұрын
Excellent video
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Thank you so much. Stay with us
@jolll2469
@jolll2469 2 жыл бұрын
Thanks a lot..but i have a question, this method is also applicable to virgin coconut oil
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Yes.
@abetguerzon1939
@abetguerzon1939 3 жыл бұрын
from your test how can i measure the amount of insoluble matter in alcohol amd water
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
It is not the test method for insoluble matter in alcohol and water.
@ujjwalkoirala6770
@ujjwalkoirala6770 9 ай бұрын
Only 2-3 drops of phenopthalein indicator is added what if we add 6-7drops.
@sonofasnitch2172
@sonofasnitch2172 4 жыл бұрын
or do we need to wait until the mixture is cooled down to room temp?
@MicroChemsExperiments
@MicroChemsExperiments 4 жыл бұрын
Son of a Snitch, you have to carry out titration when the misture is hot.
@sonofasnitch2172
@sonofasnitch2172 4 жыл бұрын
@@MicroChemsExperiments noted. thank you🙏🏻
@ImranAli-vd6pd
@ImranAli-vd6pd 2 жыл бұрын
Son of a snitch means?
@SwhyBe
@SwhyBe 11 ай бұрын
Good day is there unit for the acid value ? due he did not write it
@chegufoods9425
@chegufoods9425 3 жыл бұрын
What is the maximum value of ffa for a good oil...?
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Different oils have different FFA value. Search on google to get idea about the reference ffa value of an oil sample
@oldisgold5197
@oldisgold5197 Жыл бұрын
Can we use methylene blue instead of phenolphthalein? Then what are the procedures?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
No. You can't
@abigailabu9299
@abigailabu9299 9 ай бұрын
Please help me out, how do calculate FFA from acid value dealing with shea butter sample
@MicroChemsExperiments
@MicroChemsExperiments 9 ай бұрын
Follow the calculation given in the description section of this video
@abigailabu9299
@abigailabu9299 5 ай бұрын
What you calculated in this video is acid value, is it the same with free acid value
@irawansukma7540
@irawansukma7540 3 жыл бұрын
How to calculate the acid number of a mixture of oil? If i want the total mixture of oil is 10 gram and i have 2 oil with diffent acid number. One is 0,1 and the other 4. How much of each of these oils? Thank you
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Sorry we are unable to explain for oil mixture.
@m.sulimannessary2885
@m.sulimannessary2885 Жыл бұрын
Please tell me that the determination of Acid value with FFA is different?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Please read the description section
@fransiskarusli439
@fransiskarusli439 3 жыл бұрын
hi, how do we know the "expected acid value" if let's say I do not know the profile of the oil I am about to test?
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
If you dont have any reference expected value then test your sample twice or thrice asuming high, medium & low acid value. After performing the three tests you will get an idea about the acid value of your sample.
@IrfanAli-xy5bf
@IrfanAli-xy5bf 4 жыл бұрын
Miss why are you adding phenopthline for twice?. Also requested share procedure for rancidity test with reference. Thanks.
@MicroChemsExperiments
@MicroChemsExperiments 4 жыл бұрын
Irfan Ali, Thanks for your question. Yes adding Phenolphthalein twice is needed for this test. First time Phenolphthalein is added to carry out the neutralizing titration. Second time, it is added for the Acid Value determination titration. Both are needed because during boiling Phenolphthalein activity is lost in heat. So you have to add Phenolphthalein again before starting the Second & Final titration. We will try to upload a video for rancidity test very soon. Stay with us.
@عبداللهمهيوب-ر3غ
@عبداللهمهيوب-ر3غ 2 жыл бұрын
Please I need from you to give me the updated AOAC method If you have
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Sorry, we don't provide any document
@osharivepaul8709
@osharivepaul8709 2 жыл бұрын
I really love your easy methods. Please help with this question. Based on this method , my expected acid value is 15-75. Will it be right to use 0.5g of sample and 50 ml of ethanol?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Yes. Do it
@gauravsinghgauravsingh6553
@gauravsinghgauravsingh6553 2 жыл бұрын
Very good bhai
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Thank you
@sharonbudiongan5997
@sharonbudiongan5997 2 жыл бұрын
If my sample is margarine (is solid) do I need to melt it first before proceeding with titration?
@grebulocities8225
@grebulocities8225 2 жыл бұрын
It would help to add it to your flask. But ultimately it doesn't matter as long as it's totally dissolved in your ethanol before you do your titration.
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Correct
@PrashantPatil-hs5rr
@PrashantPatil-hs5rr 2 жыл бұрын
Something is not right I think formula molarity mentioned 40 but actual sodium hydroxide 28.2 is right
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Corrected Calculation is given in the description section of this video
@HashmiMariam
@HashmiMariam 2 ай бұрын
formula weight of NaOH is 40
@krantidippawar2254
@krantidippawar2254 Жыл бұрын
Good video, Pls unit for acidity is missing.
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Acid value does not have any unit. It is a value without unit like pH.
@rashibhasan873
@rashibhasan873 3 жыл бұрын
Please check my procedure to help me whether i am right or wrong. I at first take 99% etanol and add it to 5 gm oil sample. Then heat it for 7-10 minutes and then add 1 ml 1% or 5% phenolphthalein indicators and carried out titration Against KOH. Then a blank titration is done. Volume of KOH = KOH required for the test - KOH for blank. Say KOH for Sample = 0.8 KOH for blank = 0.2 Volume of KOH = 0.6 I then use the following formula ACV = Molarity of KOH * Volume of KOH * Molecular weight of KOH / sample weight. In this i Don't need to neutralise the alcohol as i carried out a blank experiment.
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Correct
@amtknh
@amtknh Жыл бұрын
hi is acid value depends upon the number of grams of fat taken. i take normally 1 gm of mustard oil to calculate ffa .what should be its range in that case
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
You should take 10g sample
@SohelRana-ln7bg
@SohelRana-ln7bg 3 жыл бұрын
Thanks a lot, Pls peovide FFA method & It's calculation
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
This video is also applicable for FFA test. Just FFA calculation is different. FFA calculation is given in the description section of this video.
@PsykkMusic
@PsykkMusic 3 жыл бұрын
Hi. I am completing a similar titration to measure a change in acid value of canola oil after oxidising it in a microwave, and have encountered some dilemmas. After neutralising the ethanol with my NaOH base, I've been mixing 40ml's of it with 10ml of oil, titrating it with NaOH. I did not heat the solution. For the acidification of the oil, I used 50ml of it and left it in the microwave for between 30 and 150 seconds, but when I titrated these samples, no noticeable change in titre value was detected. Will the titration actually be able to detect a change in acidity after taking the oil samples from the microwave, or did I just not oxidise the samples long enough? Also is it necessary to heat the ethanol before mixing it with the oil to dissolve it properly? Should I try a greater ratio of ethanol to oil, or will it be too much ethanol to detect any changes in the oil sample? Thank you for your time. I hate to be something of a burden but I do need a response within a day or so, but clearly I am within no right to expect that. I appreciate any efforts to assist me.
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
We never tried intentionally oxidation of oil. So we dont have any experience about it. But we think that the difference should be noticable in your experiment. Please conduct your experiment again following this video strictly. Increase the oxidation time of your sample. Dont use excess ethanol. Use 50ml nutralized ethanol for 10ml sample and heat the ethanol before mixing with sample. Dont keep any change of the method described in this video. We hope that you will find difference between two different conditions of oil.
@PsykkMusic
@PsykkMusic 3 жыл бұрын
@@MicroChemsExperiments Thank you for your quick reply and explicit instruction. I will be sure to re-evaluate my method and follow the instructions of the video more precisely, with the ratios you've outlined. Thank you very much again for your response and kind words. 👍
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
@@PsykkMusic you are most welcome dear. Waiting for your experiment results.
@PsykkMusic
@PsykkMusic 3 жыл бұрын
@@MicroChemsExperiments Sorry to bother you again, but I just had a few further questions questions that have arisen as a result of some continued investigation, if you are willing to answer them. Primarily, I noticed that no element of the formula used to calculate acid value actually accounts for the particular oil to ethanol ratio, but from my experiments, increasing the amount of oil in the solution lead to an increase in the amount of NaOH used? Fortunately, for my experiment I've found that a 1:5 ratio has yielded results that work well in the calculations thus far, but someone suggested that I should test this with different ratios to further confirm the acid value, but I noticed the formula won't actually account for the ratio. It also doesn't account for the total volume of the oil/ethanol solution. Is there a way to calculate Acid Value to account for changes ratio's and solution volume? If not, I understand. Also, for the calculation of the amount of Oleic Acid present in the sample, is the formula the same as for the calculation of the Acid Value, just replacing the Molar Mass of NaOH with the mass of Oleic Acid? If so, would you be able to give a brief explanation why? Furthermore, If you have the time, I was just wondering why the solution needs to be heated? Is it just to increase the solubility of the oil? Could the ethanol be preheated to 95ºC and then be mixed with the oil? When I heat my 60ml samples for 10 minutes on a hotplate at 150ºC, I still notice some oil not completely dissolved? Should I wait until the bubbling becomes more aggressive? I greatly appreciate your efforts thus far and hope not to disturb you too much further after these comments. I understand I ask a lot, and am grateful for your efforts. Thank you very much for your time and I look forward to your response.
@essang0428
@essang0428 2 жыл бұрын
maybe you forgot to add indicator before titration.
@rihab.jelassi.nutritionniste
@rihab.jelassi.nutritionniste 3 жыл бұрын
Hello, is this protocol available for measuring AV of ghee ( butter oil )? and can you mention the link of the AOAC please? Thank you
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
You can measure the acid value of ghee by this same method.
@killjadeskye
@killjadeskye 3 жыл бұрын
Hi, does the mixture have to be heated until boil? if so, how long should i let it boil?
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Boil until the oil is dissolved in ethanol completely
@nhdmaheswari2561
@nhdmaheswari2561 Жыл бұрын
How to know whether the ethanol is dissolved or not because my solution always separated as ethanol and oil after i removed it from hot plate
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
No problem. During heating, acidic properties comes with ethanol.
@SohelRana-ln7bg
@SohelRana-ln7bg 3 жыл бұрын
But the oil samples doesn’t mix after boiled
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Boiling of oil with ethanol will not facilitate to dissolve the oil completely. So no worries about it. Perform the test according to our video. You will get the accurate result.
@karishmasaini4076
@karishmasaini4076 25 күн бұрын
Thankyou so much for sir ji
@olasupooladirantayo9711
@olasupooladirantayo9711 Жыл бұрын
Please what is the implication of Acid value on oil quality?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
The higher the acid value and free fatty acid content, the lower the quality of the oil
@avinashmokashe527
@avinashmokashe527 2 жыл бұрын
Thanku so much ma'am 🔥✨❤️
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Most welcome 😊
@hhasheminejad1502
@hhasheminejad1502 2 жыл бұрын
Thanks a lot for sharing this very informative video. I have a question, can I replace EtOH 96% or 70% instead of 99%?
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
You can use 96% but not 70%
@udaygoud1017
@udaygoud1017 Жыл бұрын
Can you explain why you perform this test
@AidilAdrie
@AidilAdrie Жыл бұрын
sir, I'm using KOH solution for titration, is it okay for me to use methanol as the solvent instead of ethanol?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
No. You have to use ethanol
@AidilAdrie
@AidilAdrie Жыл бұрын
@@MicroChemsExperiments Thank you Sir
@SwhyBe
@SwhyBe 3 жыл бұрын
W stand for the weight of sample right?
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Yes.
@samsciences5966
@samsciences5966 3 жыл бұрын
great work
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Thank you
@lalitverma5279
@lalitverma5279 Жыл бұрын
But the molecular weight of naoh is nearly 40 so why we use 56.1 for acid value as you mentioned
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
You have to use 56.1 for both of NaOH and KOH
@lalitverma5279
@lalitverma5279 Жыл бұрын
It will be same for rice bran oil??
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
@@lalitverma5279 yes
@hind1245
@hind1245 3 жыл бұрын
Thanks so much for sharing, we dissolve sample with solvent may be (hot ethanol) or a mixture of 96%ethanol and diethyether(1:1)...and heating the sample with solvents if necessary by gentl heating about 40°c.. Because high temperature destroy triglyceride and realise free fatty acid ..
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
We didn't try this method you described. Please try our method described in our video which was made by following the AOAC strictly.
@blackbirdchannel9511
@blackbirdchannel9511 Жыл бұрын
Pls make a video on tannin in tea
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Noted
@sunitakunwar5966
@sunitakunwar5966 2 жыл бұрын
Every oil acid value ranges from 1-4 if we take 10 gm oil or different oil has different ranges ?
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Every oil has the same range
@grebulocities8225
@grebulocities8225 2 жыл бұрын
Different oils have different ranges and you need to adjust your sample weight accordingly. I work at a bioethanol plant that produces distiller's corn oil, which has a very high free fatty acid content (about 15-25). For this, about 0.5 g is appropriate. There's a chart early in the video that gives the right sample weight for different expected FFA concentration ranges.
@munishtiwari4097
@munishtiwari4097 Жыл бұрын
How to find out the free fatty acids value of rice bran oil
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Same method
@munishtiwari4097
@munishtiwari4097 Жыл бұрын
Which indicator is to be used
@shilpak753
@shilpak753 Жыл бұрын
How to do solid sample like mixes for free fatty acid procedure tell me
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Take 10g of crushed and mixed sample and do the same as done in this video.
@prathibhaperera-o5e
@prathibhaperera-o5e 10 ай бұрын
Can use this method for detect FFA in milk or milk product?
@MicroChemsExperiments
@MicroChemsExperiments 10 ай бұрын
Yes.
@amtknh
@amtknh Жыл бұрын
for 1 gm sample a cid value range expected ?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Find from google
@Iqra-ix1cf
@Iqra-ix1cf 10 ай бұрын
Thnks ❤
@cheminblack3583
@cheminblack3583 2 жыл бұрын
Hi Can you please tell me What is the difference between fatty acid and free fatty acid?
@grebulocities8225
@grebulocities8225 2 жыл бұрын
Fats are normally in the form of triglycerides, with three fatty acid units connected as an ester to a glycerol molecule. Usually "fatty acids" in the context of a fat means all fatty acid units, whether bound as triglycerides or not. Free fatty acids means just the fatty acids that are not bound to glycerol, those that are just floating around "free" as R-COOH. Those are the only ones that are able to contribute to titrable acidity.
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Yup
@jancy98
@jancy98 Жыл бұрын
Why don't you show the standardization of NaOH in this video?..isn't it necessary?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Yes. Standardization of NaOH is must. We didn't show the procedure of standardization in this video. We just mentioned that standardization is needed. We developed another video for the standardization of NaOH and already uploaded in this channel. Please find the video if needed. Thank you
@jancy98
@jancy98 Жыл бұрын
@@MicroChemsExperiments ❤️
@kktv2181
@kktv2181 2 жыл бұрын
Hi dear i have a question. all oil or dalda cooking oil what is calculation
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Same calculation
@gp5456
@gp5456 2 жыл бұрын
Dear Sir/Madam, does plastic oil also have acids and free fatty acids?
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
All kinds of oil have fatty acids
@gp5456
@gp5456 2 жыл бұрын
@@MicroChemsExperiments thank for your reply.
@AshishKumar-rf5tv
@AshishKumar-rf5tv 3 жыл бұрын
Acid value and Free Fatty Acid determination are the same or different? Thanks
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Same test but different calculation. Please find the calculation in the pinned comment or description section
@AshishKumar-rf5tv
@AshishKumar-rf5tv 3 жыл бұрын
@@MicroChemsExperiments thank you very much!!!
@AkashKumar-xp8hy
@AkashKumar-xp8hy 3 жыл бұрын
Thank you so much
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
You are welcome
@cheminblack3583
@cheminblack3583 2 жыл бұрын
Can you please explain what is acid value and why we determine this?
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
It represent the quality of oil. For more, study with the resources of google
@grebulocities8225
@grebulocities8225 2 жыл бұрын
It gives you a measure of how much of the fatty acids in a sample of fat/oil are free fatty acids, as opposed to being bound to glycerol as triglycerides. This matters for several reasons depending on the application - for instance, high free fatty acids can help oils to go rancid faster, or for biodiesel free fatty acids will be turned into soap, which will interfere with the process by forming emulsions or (if it makes it into the final product) damaging engines and fuel filters.
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