Multifactor Designs in DESeq2

[@PriyankaMehta-e7y]

Is day 7 where dispersion and other estimates explained uploaded anywhere? I would love to see know what is explained there.

[@xenial8605]

Hello! Thank you for your video this topic is definitely cause for confusion. I am stuck on chapter 8 and hoping you could clarify where my thinking error is? I have an RNASeq exp with 3 time points and 3 treatment types+a control(DMSO). I run DESeq() as below twice, once using the 24hour as a reference point and once for the 48hour. I believe I should be extracting the same result below as you explain in your video (here treatment effect "Ibr" at 48 hours), but the results differ. design

[@hhhh56hh545]

hi, very well explained, thanks. question: I have a set of data which has this condition: 1. severe drought in short time 2. severe drought in long time 3. mild drought in short time 4. mild drought in long time now, I want to make a meta data but I'm not sure whether I should make one single column which contains all the conditions and their controls or I should make separate columns for short + long + severe + mild any suggestion would be appreciated.

[@MetehanÇELEBİ-f8j]

Hey! I also have similar case. Did you find a way to design your conditions? Thanks in advance!