Mushroom Breeding - spores, monokaryons, anastomosis, and somatogamy (dikaryon formation).

[@ASJ..]

Mr Grand you and Mycophilia(sage)have and continue to teach me sooooo much with yalls phenomenal lessons on all your channels. Thank you so much.keep up the GREAT work!!! Mush Luv man😊

[@edwardgrand]

Thank you for the wonderful comment! You made my day :)

[@ASJ..]

Your very welcome. 😊

[@Mycophilia]

Great video! Nobody explains this stuff to this much detail on here. 🍄 💜

[@MissBlackMetal]

You and Gary over at Fresh From the Farm Fungi are my absolute favorites for this subject on KZbin -- pure facts, no redundant rants, and it seems like you both have academic or professional backgrounds in microbiology, if not specifically mycology, so you guys know what you're talking about and can get into the weeds with it. The latter is what really sets you two apart from the rest (most of whom are just regurgitating what they learned or heard elsewhere). As a newbie, I always learn quite a lot of new information from each of your videos, so thank you very much for making and posting them!!! I really appreciate it!!! Please keep doing what you're doing 💕

[@edwardgrand]

Thank you for the nice message and watching. I'll be doing this stuff until the day I die, which should be a couple more decades at least ;)

[@TheTubejunky]

I think Gary is doing a series on serial dilution and singling out viable spores. I am highly interested in this subject. Thank you guys for helping everyone understand our fungi friends.

[@edwardgrand]

I love Gary's videos. Too bad we don't live closer, we would make a good duo!

[@tonystewart7624]

​@@edwardgrand Hell yeah you guys would!

[@JUSTINOURSE]

​@@edwardgrandcouldn't agree with you more ‼️

[@sminstudios]

Greek etymological root for Chunky Stuff? - this is part of what makes Ed a great educational channel

[@Alexmw777]

i love your educational content. i understand that you want to keep things brief for an introduction, but don't be afraid to make longer form content that goes into more rigorous detail if you think you'd enjoy filming it. you make a great educator. i'd watch longer-form lecture style content.

[@edwardgrand]

I go into a lot more detail in the lives. I do enjoy doing 'lecture' style things, but it is quite hard in a video to go on for any length of time without it getting boring. Thanks for the suggestion and thanks for watching.

[@nathanaelhughes1865]

Always a great time learning from one of the greats! Thanks for the knowledge!

[@Somecallme_Hud]

Dude, I've been looking for this video for weeks now! Thank you so much for taking the time to educate us on this subject!

[@jedi3949]

Edward dropping knowledge no compromising on ethics.

[@Dr.salamanderslab]

I've been rewatching allot of your videos and they have been so helpful thanks for all you do Ed ❤

[@MrByukas]

Love the SFX

[@biggerstaff25]

Love learning with you Ed , you’re a great teacher !

[@diegoramosg]

Love this video. Clears up a lot of miss information.

[@cubertbensin]

I love these videos of you breaking down the more complex topics! Absolutely fantastic what you’re doing here Ed! Looking forward to the rest of our lives!

[@nikiel666]

🎉thank You

[@chuckheppner4384]

Keep on keepin' on, Ed. Thank you for your generosity.🙏🏻 You have to struggle to stay alive and be of use as long as you can." ~ Daniel Berrigan

[@OldBrownWookiee]

Oh yeah!!! Happy to watch these!! Thanks for the content brother!!

[@SmallMouthNoises]

The GRAND DRAG! I dig it 👌

[@patnowhere]

Starting to put all this together in my head! Thank you!

[@ANJH1106]

Great stuff hereove the breakdown spread the knowledge!

[@arctictimberwolf]

Thanks, that makes sense now. I have a small 100x-450x-750x monocular non-digi Microscope: Do you think a 750x Microscope is good enough to see the spores in the different states? Thanks Ed, have a great day!

[@edwardgrand]

For sure. 450 or 750X is plenty. I usually look for clamps (or lack of) at 400X. No problem. I hate going to oil at 1000X. Messy.

[@Basidium_King]

Thank you soooooo much………. Please keep bringing the knowledge

[@jeremyhague1860]

Appreciate the knowledge!

[@tonystewart7624]

Thank you for the break down Ed!

[@headcheese2005]

Thanks for the lesson!

[@calebtot]

....What? Just kidding, great video! That cleared up a lot for and also gave me plenty new roads to go down learning wise! Awesome! Thank you. Right before this I was staring at a plate with a ton of different splotches where the spores have germinated thinking.... so I just pick the one I like and transfer it? This really helped me to gleam a bit more insight as to what was going on on the plate. I'l definitely do a few good transfers to simplify the genetic makeup.

[@TheTubejunky]

I have inoculated another like on this channel.

[@sminstudios]

I turned a monolike comment into a dilike

[@xxdustinadamsxx]

I appreciate this video so very much. 🙏 I also will need to watch another 47 times just to get it to stick, buuuut I appreciate it nonetheless 😂

[@gangsterbuilder]

Thank you

[@maybebanana749]

As always, great and helpful. How close do the genetics have to be to “merge” 2 spores? Like could it be an active and an oyster? I am guessing not, otherwise we would see them around?

[@edwardgrand]

They need to be closely related. Like a horse and mule, not a cat and dog. You're right. If you could do it, someone would have already.

[@sminstudios]

For the record, I’m calling “it” the Grand Tour Method. It explains itself. BONUS we can blow people’s minds in 2026 informing them it was named after this cool guy literally named Grand…

[@sminstudios]

Oh of COURSE I mean the “Grand Tour TEK” please forgive me

[@Jaime-x2f]

I'm a dinosaur and am here to learn. If I misbehave let me know . I ordered spore syringes and got 3 kinds most of the boxes looked like normal shrooms except 3 boxes turned out to be blobs. They look like sea shells. I never saw anything like it then I saw you had some too. Are these accidents. One blob was 200 grams wet. Do they work? Is it a good thing?

[@edwardgrand]

Blobbing is a real thing, but no one seems to know why they do it. Sometime genetic, sometimes environmental. I am just trying to pull out some of the blob genetics using the Buller phenomenon (di-mon pairing). If you could figure out why they do it, I'm sure a lot of people would love to know :)

[@aldoevortega5224]

First view 💪

[@edwardgrand]

Let me know if I said anything stupid 😉

[@diegoramosg]

Hi Ed, Would you be able to do a quick video explaining why infinite cloning degrades the genetics of the mushroom, or is this more misinformation?

[@edwardgrand]

There are a lot of opinions on this. I don't have the definitive scientific answers. For the home cultivator, it doesn't matter.

[@chemd1767]

If there happens to be an odd number of manokaryon colonies, what becomes of the odd/remaining manokaryon?

[@edwardgrand]

On an agar plate, the dikaryon will most likely outgrow the two monos. The dikaryon usually shows rhizomorphic growth which is isolated to a new plate and transferred repeatedly until it hopefully becomes a 'monoculture'. There are many variations on this theme though. In the verified mon-mon mating scenario, there is only one possible outcome for the dikaryon which forms as the monos will not dikaryotize with themselves. This is why multi-spore 'double swabbing' methods are more problematic. In a mon-mon cross, there should never be an odd number of monos. There should only be two on the plate.

[@stefonwitten8164]

You have very nice handwriting for a doctor, Ed! 😂

[@edwardgrand]

I'm not a real doctor, I only play one on TV ;)

[@stefonwitten8164]

@@edwardgrand 😆

[@klintburk2960]

All smart ppl write sloppy!! Lol genius can explain it while it’s not legible!! Lol ❤

[@kyledejesus9024]

up 4:19

[@Arcanefungus]

Pollen is not a gamete, its the gametophyte

[@edwardgrand]

Yes. I'll let you explain that one ;)

[@kyledejesus9024]

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