Mutagenesis and/or an attempt to make a polyploid using colchicine. Basics of experimental design.

[@h-townwolf3910]

very interesting and above my knowledge base. Thanks for breaking this down, Ed

[@oldboots75]

Cheers for the video Ed and thank you for using the lapel mic👍

[@violentclimax5722]

Found your vids a few days ago and I'm almost done with all of your vids ... love the format and info you share... shame this is the 1st vid where I understand verry little of your terminology and practice.. time to hit the reaserch again thankyou for making me wanna up my game even further... please keep up the good vids dude (also would love to hear some cannibal corpse in the background) x

[@edwardgrand]

I might have to redo some of the early videos with my new mic. I'll try to go into more explanation about the terminology. Please let me know if you have any questions and I can do a whole video on that particular topic.

[@beekerdabs4996]

Another great video as always thanks u are such a wealth of knowledge F@#k around n find out 4 the 🏆

[@RawTubeTV]

Sound is good, Ed! Good night!

[@plazamidwoodpainting]

Great video, ill be reaching out on IG about a project. Maybe you can quote us a price. Thank.

[@nicolasavilarodriguez1290]

amazing work!! keep it up bro! it would be amazing if you could share some of those articles you are reading .

[@mycoGeeky]

Just happen to have colchicine handy huh?

[@edwardgrand]

Yeah. I was gonna poison someone. LOL Aging sucks.

[@chemd1767]

🤣🤣

[@ByronBTB]

You can buy the plant that produces it easily enough...

[@elced01]

Colchicine generally used in a liquid culture for a few days for plants. That how it is done for microspores to create double haploids. It has been show to work in some mycelia but not others.

[@epidemicasmr3968]

Did you sterilize the colchicine solution in an autoclave prior to adding to your media?

[@edwardgrand]

I sterilized the colchicine in the autoclave for 30 minutes after it was dissolved.

[@epidemicasmr3968]

@@edwardgrand 15 psi? Did the heat/pressure negatively impact the colchicine? Did you have any readable results from this? - thanks for your swift reply!

[@edwardgrand]

@@epidemicasmr3968I'm not sure what the results where. I never got around to doing the nuclear staining. Too busy these days with other projects. I might try Fluconazole in the future also.

[@epidemicasmr3968]

@@edwardgrand No worries - thanks again for the reply - subbed!

[@LtDan-co5tk]

Very exciting! Let us know either way. Audio was much better! 😆

[@nikiel666]

🎉

[@JoeMushrumski]

Could you possibly use Methylene blue or eosin?

[@edwardgrand]

Thanks. I will try. Never done it with fungus nuclei.

[@JoeMushrumski]

@@edwardgrand No worries. Hmmm I had another reply there with links to a couple papers. Did you see it?

[@edwardgrand]

@@JoeMushrumski I didn't see it. I'm kind of losing track of stuff.

[@LtDan-co5tk]

Sending polyploid vibes ur way 😂

[@KS-jz4qq]

Any luck with this experiment? I am very interested in hybridization between species and possibly genus in gourmet and medicinal mushrooms. Great work on your vids!!!

[@edwardgrand]

Making spawn for both the control and colchicine treatment now. Inter-generic hybridization...woo...good luck.

[@1871corporationUSA]

​@@edwardgrandthat stuff should be ready by now😉

[@edwardgrand]

@@1871corporationUSA I've fruited it. No noticeable mutations. I think the concentration of colchicine wasn't high enough. You can't buy pure colchicine though because of it's toxicity.

[@justinfitzgerald7802]

Mic worked great!

[@HASHHASSIN]

and results?

[@edwardgrand]

I got bored staining nuclei. Null results.

[@HASHHASSIN]

@@edwardgrand can you teach us how to make Polyploid Cannabis plant with Colchicine? :) Cheers from overseas. Great videos btw.

[@edwardgrand]

No, but there is plenty of information in the Nepenthes literature.@@HASHHASSIN

[@HASHHASSIN]

@@edwardgrand any specific paper(s)?

[@edwardgrand]

No. Just Google search stuff.@@HASHHASSIN

[@patnowhere]

Like Data from the Goonies you were saved by the Pitchers Lapel..

[@psilocyborg4775]

Ohh my god I know that movie word for word and never knew what Data said in that scene🤣 I used to say pitch as a pal as a kid🤣 thankyou 🙏

[@Jen-br5hx]

wow. i never thought this ould be applied to fungi, im aware of its use in cannabis