My Philodendron Pedatum Variegated Got Contaminated! | Resterilizing Tissue Cultured Plants

In this informative and hands-on video, join our lab director, Francisco, as he takes you on a journey of resterilizing tissue cultured plants. In an unfortunate turn of events, Francisco's beloved Philodendron Pedatum Variegated has fallen victim to contamination, but fear not, as he shares his expert knowledge and techniques to revive and restore its health. (Use this link to save 10%off your first purchase: www.plantcelltechnology.com/?... )
Contamination in tissue cultured plants can be a common challenge, often resulting from the presence of unwanted microorganisms. Francisco, with his vast experience in plant biology and laboratory techniques, showcases step-by-step methods to effectively resterilize your precious plants and ensure their successful growth.
Throughout this video, Francisco walks you through the process, highlighting the tools and materials required, as well as providing insightful explanations and tips along the way. From creating a sterile environment to using sterilizing agents, he demonstrates the importance of maintaining strict cleanliness protocols to safeguard the health of your tissue cultured plants.
Francisco shares best practices, outlining potential pitfalls to avoid and sharing troubleshooting advice to address any challenges that may arise during the resterilization process.
Whether you're a seasoned plant enthusiast or just starting your journey into tissue culture, this video is a must-watch. Francisco's passion for plants and dedication to their well-being shine through as he imparts his wisdom, empowering you to confidently tackle contamination issues and revive your tissue cultured plants.
Join Francisco in this invaluable tutorial and embark on a journey of resterilization mastery that will elevate your plant propagation skills. Subscribe to our channel, hit the notification bell, and be sure not to miss this educational episode on resterilizing tissue cultured plants. Get ready to bring your beloved plants back to their flourishing state with Francisco's expertise by your side!
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We are Plant Cell Technology, a leading plant tissue culture industry organization since 1995. We develop, produce, and market innovative solutions for global plant tissue culture operations and hobbyists. Our well-researched, certified, and trusted products keep your cultures healthy and contaminate-free.
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[@hxx117]

That's another one of the many reasons why I support Plantcelltech's content: they see an opportunity to give us top quality, educational content where others see a coarse batch of tissue culture to be discarded. Francisco has become the mentor of many of us without knowing it!

[@PlantCellTechnology]

Thank you so much for your kind words!

[@jonsutton5864]

Could a cannabis nodal explant be dissected for meristem?

[@jonesrdh7170]

Did you put the meristem culture in the same formula that you were using originally

[@kennyboob1337]

What are the light requirements??

[@sergiohr1850]

Just what I was looking for

[@PlantCellTechnology]

Amazing!!

[@nakamurajinusuke]

can you show us how to germinate seeds in ms and using pgr? or how to tissue culture with seeds?

[@PlantCellTechnology]

Sure! We'll inform you when the video is uploaded on the channel on this subject.

[@MsBawarka]

What microscope do you use? Great video

[@PlantCellTechnology]

Digital LCD microscope

[@dinaermias6792]

Why do you prefer isopropyl alcohol versus ethanol?

[@PlantCellTechnology]

Much cheaper and easier to find. I can go to any grocery store or pharmacy and get isopropyl alcohol.

[@7munkee]

Unless you were working under a flow hood or other negative pressure device, you did not sterilize. The split second you expose that culture to open air it is contaminated again. What you did was sanitize it. I work with fungal cultures, and it is EXTREMLY difficult to retain sterility even with a commercial flow hood.

[@claytonhigh282]

The entire bench he's working on is a flow hood. No need to be condescending, he's a professional who "works with fungal cultures" as well