Onion peel cell experiment. (Procedure in description box)

AIM
To prepare stained temporary mount of onion peel cells and to record observations and draw labelled diagrams.
MATERIALS REQUIRED
Onion, plain slides, coverslip, watch glass, needles, forceps, brush, blade, safranin, blotting paper, glycerine and compound microscope.
THEORY
Onion is a multicellular plant. Like other plant cells, the cell of onion peel consists of a cell wall, cell membrane, cytoplasm, a large vacuole and a nucleus. The nucleus lies at the periphery of cytoplasm and vacuole is located in the centre. Presence of large vacuoles and cell wall confirms that cells of onion peel are plant cells.
PROCEDURE
Take a piece of onion and bend it to remove the transparent membranous structure called onion epidermal peel. With help of forcep remove the peel from its inner side.
Place the peel in water in a watch glass.
Add a few drops of stain safranin, to the watch glass containing the peel for staining.
Now, wash the leaf peel with water and transfer it on to a clean slide with the help of brush.
Remove extra water from the slide surrounding the peel with the help of blotting paper.
To this slide, add a drop of glycerine over the peel and place the coverslip in a manner to avoid entry of air bubbles.
Soak away the extra glycerine with blotting paper.
Examine slide under the microscope.
OBSERVATIONS
A large number of rectangular cells with distinct cell wall can be observed.
Cytoplasm is seen as thin layer of deep coloured substance on inner surface of cell wall.
A big central vacuole is present in the cell.
A deeply stained round body called nucleus is seen in each cell.
RESULT
The epidermal peel of onion comprises of rectangular shaped cells. Each cell comprises of a nucleus, a central vacuole, thin layer of cytoplasm and cell wall.
As cell walls and large prominent vacuole are present in each cell, the cells placed under observation are plant cells

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For excess safranin use blotting paper and don't touch the leaf with blotting paper.

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You missed to add glycerine over Peel and put a cover slip so that air bubbles don't go in . *Just to tell you missed 1 point from my experiment in book and all are there* thanks for this now i thing 😀 my practical exam Is easy Which I am going to do for 1st time

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wash extra stain with distilled water and use paint brush, and put cover slip at angle of 45 degree.....

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Why haven't you used Janus green after safranin? We have learn that after safranin we have to stain with Janus green for plant cells. Please explain this

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How much magnification did you use to see the cells??

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I'm not a science teacher and I am tasked to teach science to jhs students. My students and I both try to explore the microscope. I'm proud that my students was able to view the image of the onion cell under the microscope. ༎ຶ‿༎ຶ༎ຶ‿༎ຶ༎ຶ‿༎ຶ༎ຶ‿༎ຶ

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Why was it stain with the iodine

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This is safranin stain safranin will stain the cellulose of cell walls making it appear pinkish red.

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Glycerine ka bhi use hota hai

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When we made slides we added methyl blue

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Safranin stain

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Question sir/ma'am. What can be used as a substitution for safranine?

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Iodine

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Another question, sir. What could be an alternative for glycerin?

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@@SomeoneNevermind glycerine easily available in medical store

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Last question sir. I went to different medical store yet all they have is glycerin suppository. Would it be alright for the lab experiment?

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