Origin of Replication - Plasmids 101

Working in a molecular biology lab, you may be expected to replicate plasmids. But to do that, you’ll first need to understand the mechanisms behind it all. What is it that drives plasmid replication? The answers lie in the Origin of Replication.
blog.addgene.org/plasmid-101-...
Our Plasmids 101 videos are designed to educate all levels of scientists and plasmid lovers. For more, blog.addgene.org/topic/plasmi...
0:00 Intro
0:24 The Replicon
1:07 The Makeup of Plasmid Oris
1:34 Bacterial Plasmid Oris
2:59 Plasmid Selection Guidelines
3:36 Replication Guidelines
5:22 Links and Additional Info
__________________________________________________________________________
Credits:
Written, Directed & Animated by: Quintin Marcelino
Based on Previous Material by: Kendall Morgan and Leah Schwiesow
Designs and Illustrations by: Jason Snair
Additional Designs by: Quintin Marcelino
Narration by: Quintin Marcelino
Sound Design & Sound Editing by: Quintin Marcelino
Special Thanks to: Jennifer Tsang, Aliyah Weinstein, Leah Schwiesow, Joanne Kamens, Alyssa Cecchetelli, and everyone at Addgene who provided support and feedback on this project.
Music by: Slynk, Kwon, Dan Lebowitz, and The Grand Affair, courtesy of KZbin Audio Library
Game Sound Effects by: Little Robot Sound Factory

[@addgene]

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[@akshitadixit1011]

What is the exact definition for copy number?

[@addgene]

It's the number of copies of a plasmid in a given cell. The table in our article provides the average number of plasmid copies per cell, however the actual number can change depending on other factors. For further reading, you may enjoy BiteSize Bio's article, How to Manipulate Plasmid Copy Number. bitesizebio.com/22824/how-to-manipulate-plasmid-copy-number/

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