Ouchterlony Assay

For more information, visit www.bio-rad.com/yt/6/biotech-lab-textbook.
This video demonstrates the Ouchterlony double immunodiffusion assay. This technique is used to test whether antibodies recognize specific antigens. This activity is included in the laboratory textbook Biotechnology: A Laboratory Skills Course.
www.bio-rad.com/evportal/destination/product?catID=LKN6R715&WT.mc_id=yt-lse-ww-biotech-20121012-Fnx5CkGRBEM
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[@t-alimichael3363]

Would be nice to nice the end result. Thanks for sharing!

[@liniencemaposa5479]

Good answer from admin. Igs are proteins and our skin too, so wearing glooves is part of GLP.

[@lynok55]

Thankyou. Very clearly demonstrated.

[@alaamansour6785]

I know it's too late but can u tell me the meaning of the shape of the line formed after incubation please. It may be straight | or curved like this ) or that ( , I can't understand these (, ) so please tell me^^

[@alaamansour6785]

***** Ah I see but we were told that if the line is like that ( or ) it may indicate the concentration of the Antibodies in patient's serum and the Antigen *which is one of streptococcus groups A,B,C,D and E* is that right? We didn't study that test in details that's why I can't understand it :( I'm really sorry if I sound stupid :(

[@nehanagrale9994]

Which liquid is added in negative control??

[@oumani12]

thx

[@nadeemimdad8186]

Good

[@jah0524]

Protein concentration?

[@EDUARDO12348]

Sample A tube did not close right... its going to get contaminated....oooo my god.....lol jk thank for video. Interesting to know that this simple techinque has now become standard except its in test strip form.

[@hazardmitigation8692]

To the contrary. People who always wear gloves when handling laboratory reagents are often people who have not thought through the potential hazards. They're often the most ineffective and dangerous as laboratory personnel because they have not thought through the procedures sufficiently.

[@NorthForkFisherman]

Thinking through the procedures is fine, and definitely recommend. However, you also assume that everything in the lab will be correctly labeled and not be a potential hazard. Treating the lab and any reagents found within respect at all times is definitely best practice.

[@eltiletulf5049]

youre probably dead by now lol

[@hazardmitigation8692]

Why is the demonstrator wearing gloves? I don't see anything hazardous in use.

[@johnwilloug2717]

...not the neurotoxin acrylamide? I would always wear gloves with these gels. While only unpolymerized polyacrylamide is supposed to be a problem, who would bet on it being 100% polymerized? I wouldn't.

[@amlam1999]

@@johnwilloug2717 preach.