pcr explained | pcr in hindi | polymerase chain reaction in hindi | pcr techniques in biotechnology

PCR (Polymerase Chain Reaction)
इसे पॉलिमरेज शृंखला अभिक्रिया भी कहते हैं। इस अभिक्रिया में उपक्रमकों (छोटे रासायनिक संश्लेषित अल्प न्यूक्लियोटाइड जो DNA क्षेत्र के पूरक होते हैं) के दो सेट्स (sets) एवं DNA पोलीमरेज एन्जाइम का उपयोग करके पात्रे (in vitro) विधि द्वारा लाभदायक जीन की कई प्रतिकृतियों का संश्लेषण होता है। यह एन्जाइम जीनोमिक DNA को टेम्पलेट के रूप में प्रयुक्त करके तथा अभिक्रिया से प्राप्त न्यूक्लियोटाइडों का प्रयोग करके उपक्रमकों को विस्तृत कर देता है। यदि DNA प्रतिकृतियन प्रक्रम कई बार दोहराया जाये तो DNA खण्ड को लगभग 1 अरब गुना परिवर्धित किया जा सकता है।
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PCR Polymerase chain reaction

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