PCR Primer Design

[@macielrodriguez6697]

Thank you so much!!! Your explanation really clarified a lot of tinny doubts that I was afraid to ask to any of my teachers! But you explained all so well and soo clear!!! Thank you so much!

[@prasannaweeratunga6158]

This is probably the best example out there for anyone trying to understand premier design...... Indeed Mr. R Kanda....... Way to go Glasgow.....

[@satishraina4434]

Have seen over a dozen videos on this topic, nobody has put it as simple as you have.

[@licious4633]

I love the way she explains everything very simple and clear.I wish she was my professor.She inspires me alot! I just wish I was working as her assistant.

[@Rogis313]

oMG I LOVEE YOU, YOU HAVE JUST EXPLAINED 2 MONTHS OF CLASS IN 10 MINS, ALL MY QUESTIONS HAVE BEEN ANSWERED THANK GOOOD

[@aimanraza]

This was strangely relaxing. Kind of like ASMR lol

[@joewheeler3210]

Could anyone else listen to this before bed ? Most relaxing thing ever

[@RKanda]

This is probably the best example out there for anyone trying to understand premier design!!!!

[@steveholmes161]

Absolutely excellent explanation. So clear and concise. Thank you!

[@swinkadoo]

Thank you for your help. The beginning of your explanation is what made it clear for me , when you delineated the 5 to 3 directions of the primers, because from there, it is easy to see which complement strand is necessary. Brava

[@prashu1585]

Thumbs up if you noticed that she introduced mutation while designing reverse primer at 8:05 minutes

[@celiasantos5975]

Yes she did!

[@mohannadkhader7911]

yes she did (T-C)

[@Biomeducated]

Sneaky! :D

[@konanfarrelly-horsfall9919]

Whoops... a deletion in this gene would cause a frameshift mutation haha

[@k.c.george6993]

Thank you very much. Beautiful, clear tutorial. Answered all my questions at once!

[@ishakuharuna5661]

Ma'am...the way you explained this is simply amazing, you have no idea how much insight I got watching this video. Please, any chance you could do another video on how to analyze the primers designed using DNAMANN or NCBI just to make sure all the primer design criteria are met?...I'll really appreciate that.

[@aligacho]

perfectly explained and saved me for next meeting with my PI

[@oswaldmens]

You're awesome. I don't think anyone can explain it better.

[@MasayoMusic]

The points at the end were very helpful . Thank you.

[@ossielmais]

I'm falling for this woman and I can't control it

[@suleibautista7807]

I was feeling dumb today at class ´cause I couldn´t imagine how the dna strands were located, now everything it´s crystal clear, thank you. You have beautiful eyes btw =)

[@AyazSamo]

So how is it with genetics and you nowadays?

[@aafreenkhan4845]

The topics are explained so well... Aren't you making more videos ?.. is there any other channel or website where I can watch your lectures?...

[@ennairam3360]

How do I avoid primers to self-complement in both cases?

[@tazagul9207]

Ur lucture is so beautiful as u are....thank u good method for teaching the people related to this topic.....love.....

[@mroosie7488]

I’m not a fan of PCR but this explanation is great.

[@oscarkee8074]

Thanks!This make me have a clear vision on study!

[@dannielleayers1731]

Clear explanation! Thank you!

[@ItsAnnaYay]

Thank you so much for this video. It has helped me so much, I understand it so much better now!

[@sarawuthyodthong2253]

Going to take an exam next weeks and this help me a lot. Instead of Primer 3 the using handmade primer is also interesting.

[@Biomeducated]

2:14 *When the forward primer IS straightforward* ! :D

[@mayercalma1810]

Very straightforward discussion. Thanks!

[@bakopala]

Thanks lady. You have saved my life

[@amalya94]

I don't understand from the 3rd example. Why is the forward primer sequence similar to the DNA sequence? Please explain to me =)

[@MrPushupking]

Thank you for this video. It really help me to understand primer design.

[@chibuzoikediugwu8453]

Thank you. Please how do you know where to start the reverse primer?

[@rahmanuddin6700]

Thanks for sharing this video. Its quiet clear and helpful to me.

[@elizebethla]

Great content! So clear and helpful.

[@santelmo5222]

Can you please be my professor for every class?

[@benjiprice8822]

yep, there`s a mistake in the reversed primer in third example. Good video, nice and patient lady, great accent, wonderful!

[@aaronnerella6907]

Amazing, this was so helpful

[@MrGoblin60]

Och aye! Good presentation.

[@purushothammabbu1364]

Nice explanation Madam.... Thank you

[@jasperobico1459]

Very well-explained! Thanks so much for making this teaching video.

[@TheSnuggleFairy]

You got the third reverse primer example wrong. You missed the double 't'! But otherwise, a good video.

[@mervebaldan7656]

Thank you for your expresion :) , Is the temperature between primers important ?

[@l.fsader6021]

Everything is important. Pressure, Volume, electrostatic charge, electric potential, electric force, affinity, and temperature.

[@zorbasg1001]

Dear teacher. I admire your scientific mind and the decency which you radiate. I seriously invite you in Greece for scientific discussion and vacation, the land where western science was born. I am involved in diseases and laboratories. Will be honor to meet you in person. I wish you the best and thank You so much for the informative excellent video.

[@ammarahmehak9986]

Good

[@mattnimbs6746]

Great video - many thanks for posting

[@THE______TRUTH]

So it's just safe to say that both primers simply need to bind to the three prime end of the DNA strand.

[@mohanjerome1753]

Thank you, you explain so well! I didn't understand half of this in class! Thanks again :-)

[@carlosvasconcelos4463]

You saved me! Why did my teacher make it sooooooo complicated?

[@lucyzhang5127]

This is perfect!

[@hayden24ful]

this is a awesome explanation, really helped! Tk u

[@marcelosantanna8726]

Excelent explanation, the best.

[@skateman296]

glasgow unaaaaaaayy!

[@Biomeducated]

Love that accent! :D

[@neha0029]

What if the gene you are looking for is on the negative strand would you then have to design primers for the negative strand?

[@buddhimaperera6273]

@Amalia safiee - Only the sense strand or plus strand of DNA is shown in that example and the forward primer in this case is elongating the other strand .

[@mikehunt3426]

I was wondering... What would happen if the two primers were on the same strands ?? Would there be any PCR's product or nothing ?

[@katiewallis9875]

PCR needs a double strand, which is why it requires DNA or cDNA. If you put a forward and reverse primer on the same strand if anything were produced it wouldn't be functional. Remember the overall goal of PCR is to get a lot of replications of your DNA.

[@mikehunt3426]

Its what i though. The product wouldn't be functional or predictable ! Thank you !

[@hitoridesulofi7188]

Thank you ! It was very helpful.

[@guizmosbr]

so good!

[@sashanewyork1966]

I think reverse primer you missed one t, there were two aa

[@tunerd1921]

Thank you so much, this was very useful

[@rajghosh4485]

Amazing

[@TaleofSunshine]

Thank you so much

[@l.fsader6021]

Are you just picking a random row to show where the reverse primer is just to illustrate the examples?

[@ranger255]

Thank you, this was very helpful indeed. :)

[@Monsterchief300]

This is a good video but it got me a little confused. Forward primer is compliment of template strand and Reverse Primer is reverse compliment of template strand right?. The reverse primer you showed at 7:45 makes perfect sense but the forward primer you should earlier for the same example is identical to the sequence. How does that make sense? The strand that is always shown, sometimes only, is 5 to 3 so isn't the primer annealing to that strand AND therefore must be compliment? Could someone tell me what I am overlooking.

[@PhysioRory]

Monsterchief300 Hi, the forward primer binds to the strand which usually isn't shown (3' to 5'), and because it is complimentary to the strand it binds to, it is the same as the top 5' to 3' section. The reverse primer binds to the strand usually shown (5' to 3'), so it is complimentary to that strand, but backwards.

[@Monsterchief300]

I finally get it now. I was focusing too much on the top or 5' to 3' strand, thinking the primer pair annealed only to this. Knowing this now il try to clear some confusion I had with coding direction given on NCBI and designing primers on Ape.

[@gautamnisha7193]

Clear xplanation thankyou

[@DrWho007]

Awesome!!!!!!

[@amyrose4434]

Good video

[@LaurenzMatter]

Shouldn't the Primer include uracil instead of thymine?

[@immunglobuling8777]

Laurenz Matter no U is only introduced in mRNA synthesis. For DNA duplication T stays T

[@JacintaColvin]

Thanks this is really helpful

[@Aug9nblick]

Very helpful ! thanks

[@panagiotisgeorgiou]

what is the annealing efficiency?

[@zamh1376]

Thank you very much!

[@viniekouamou8188]

Great teachings

[@amelya_theempatfamily7755]

thank you very much. this video was very helpfull

[@darpanmanmode3606]

very well explained

[@iViewVaranasi]

why to introduce a mutation??? why did you added a mutation ?

[@Gthrylos]

Thank you!

[@waleedgondal4672]

great

[@afifalaraib9159]

thnk u for sch a helpful video :)

[@LunaDanu1]

very helpful, thank you very much

[@Rogis313]

One thing when designing primers, what if you already have start and stop codons at the ends of the templates? I start and ATG AND TGA IS already there. Do i disregard it?

[@katiewallis9875]

PCR is essentially man-made REPLICATION. Start and stop codons are only read or put to any use during translation where you're making a protein.

[@ucheakoma863]

Educational unintentional asmr

[@martinfederico7269]

excellent

[@manvendraverma1810]

for singal strand dna if the FP sequence is same that of dna that how will it bind to dna to 5'?

[@craftsandscience860]

If I got your question correctly, the forward primer which goes from 5' to the 3' direction, bind to the single strand DNA that goes from the 3' to the 5' direction.

[@craftsandscience860]

+Easy Experiment 101 the forward primer sequence is complementary to the single strand DNA sequence

[@AK-dz8cc]

Thank you!!!

[@GuillesTheme]

Great. Thank you

[@flutteringberret7327]

Thanks

[@rawaisfandiyar9395]

Thanks alot

[@ThePestezinha]

thank you , this video was very helpful

[@omorugged1]

Good video...guys dont blame your teachers...we understand molecular biology could be difficult if attention and interest is not invested

[@robertaannibale1522]

thank you

[@tauseefkiani-]

Scottish accent ❤

[@JyotiMishra-pn2eq]

hw can i download this.?

[@RMH1Rockah]

This woman is a sain, biotech test tomorrow.

[@ultrathinking6603]

Thank u maam

[@lewischisengele8628]

i need help designing primers for Cashew powdery mildew pathogens

[@owuorgpo]

nice

[@mondayogbike132]

I Love You. just what I've been looking for....

[@dineshwellawa4872]

good

[@ntandomkhwanazi6711]

in designing primers specifically for the forward, dont you start with the start codon which is ATG?