Phase contrast microscopy principle and working

Phase contrast microscopy principle and working - This microscopy lecture is going to explain the Phase contrast microscopy principle and working in details. Phase contrast microscope is widely utilized in the imaging of small specimens. Phase contrast microscopy is a type of light microscopy that is used to visualize biological samples. This video explains the following topics in detail:
1. Phase contrast microscopy principle
2. Phase contrast microscopy working
3. Phase contrast microscope structure and set up
4. Phase contrast microscope principle and working
5. Phase contrast microscope uses
6. Applications of phase contrast microscopy.
So, if you want to know more about the Phase contrast microscope and phase contrast microscopy techniques working principle in detail, stay tuned to this microscopy lecture.
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That's a great video, but I think different shades in the image are due to interference between diffracted (from specimen) and undiffracted light from source, not due to speed difference (which I think is constant).

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Sir, here objective lens allow both S wave and D wave?? Because in dark field microscope objective allow only D wave. Annular ring is common for dark and phase microscope??

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amazing explanation. thnkyou sir for making such useful content. Sir can you please let me understand that if phase plate slits do not allow light to pass, then how can diffracted light pass through even slowly? It must not even allowed to enter...kindly please help with this. thankyou.

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one quick question. is the specimen/sample covered with a cover slip or they are left on the slide jxt like that

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And also does the surrounding wave pass through the objective lens?

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Sir in an previous lecture on Phase contrast microscope of you we saw that the diffraction light going through the inner side of surrounding light but in this video we see that it is going through the outer side of surrounding light.... Which one is correct sir?

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Sir i have a question In phase contrast microscope the specimen is stained or not and the background is bright or dark?

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So are the refracted waves part of S waves?

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I suppose in phase plate the difference is in optical density? Please confirm

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