Plant Biotechnology - 14 || PCR and it's application

#agrigpb
#biotechnology
#biology
#pcr
#amplification
#dnareplication
What is PCR?
Polymerase chain reaction (PCR) is a thermodynamic as well as enzymatic process of production of billions copies of DNA from a single segment of DNA (µg).
Kary Mullis in 1985 invented PCR which revolutionized the field of molecular biology.
Kary Mullis demonstrated that oligonucleotide primers could be used to amplify specific segment of genomic DNA or cDNA.
Mullis was awarded with Noble Prize in chemistry in 1993 for his contribution in development of PCR.
Requirement of PCR
Template DNA which is to be amplified.
Nucleotide primers of about 20 bases long (Forward and Reverse both).
Free nucleotides called ddNTPs (dATP, dGTP, dTTP and dCTP).
A heat stable DNA polymerase e.g., Taq (Thermus aquaticus) polymerase, Pfu (Pyrococcus furiosus), Vent (Thermococus litoralis).
Note : Pfu and Vent polymerase are more efficient than Taq polymerase.
Application of PCR
Gene fragment amplification as a quick alternative to cloning.
DNA fragment modification
Sensitive detection of harmful microbes, followed by accurate genotyping if desired.
Archaeological specimen DNA analysis.

DNA Fingerprinting and Marker assisted Selection
Detection of transgene

[@dr.rameshkumarmaurya4073]

Thank you sir 💐💐🙏🙏

[@agrigpb]

What is PCR? Polymerase chain reaction (PCR) is a thermodynamic as well as enzymatic process of production of billions copies of DNA from a single segment of DNA (µg). Kary Mullis in 1985 invented PCR which revolutionized the field of molecular biology. Kary Mullis demonstrated that oligonucleotide primers could be used to amplify specific segment of genomic DNA or cDNA. Mullis was awarded with Noble Prize in chemistry in 1993 for his contribution in development of PCR. Requirement of PCR Template DNA which is to be amplified. Nucleotide primers of about 20 bases long (Forward and Reverse both). Free nucleotides called ddNTPs (dATP, dGTP, dTTP and dCTP). A heat stable DNA polymerase e.g., Taq (Thermus aquaticus) polymerase, Pfu (Pyrococcus furiosus), Vent (Thermococus litoralis). Note : Pfu and Vent polymerase are more efficient than Taq polymerase. Application of PCR Gene fragment amplification as a quick alternative to cloning. DNA fragment modification Sensitive detection of harmful microbes, followed by accurate genotyping if desired. Archaeological specimen DNA analysis. DNA Fingerprinting and Marker assisted Selection Detection of transgene