Pyrosequencing

This lecture explains about the pyrosequencing technique. This is a type of next generation sequencing technology that explains the high throughput DNA sequencing method to know the DNA sequence. This lecture explains pyrosequencing steps in details.
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[@abhishekbardhan8125]

Hi, I am a great fan of yours as you are doing an awesome job and your videos really help me a lot. But still I have to make this comment so that a misunderstanding does not take place. In the video at 8:28 you said by mistake that ATP converts luciferin into luciferase which is not true. Rather ATP converts luciferin into oxyluciferin that gives luminescence in presence of luciferase enzyme. I know you must have a clear idea about that and you probably had a slip of tongue (because later you only said from luciferin, light is produced with the help of luciferase). So, if you could kindly mention the correct information in a pinned comment or in the description then I think no further confusion will take place. Cheers! And keep teaching us!

[@annsond5824]

precisely!!

[@ashwanipandey5891]

Thanku sir

[@MuhammadIslamag]

Another mistake APS is Adenosine phosphosulfurase

[@subhankarraha4033]

@@MuhammadIslamag adenosine phosphosulfate, as it is a substrate and the enzyme that converts these ppi and substrate into ATP is ATP sulfurylase. Thank you

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By far the most simplest video of pyrosequencing I've possibly ever seen. You make us students understand such complicated topics with such accuracy and ease. Thankyou so much. Keep Uploading Content Sir. You are legit a blessing to the science community

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You are a legend, explain everything so clearly! Thank you so much, you are saving me in my exam!

[@HiTricks]

Watching this video 4 years later in 2020 is a bliss. Finally was able to grasp the idea of Pyrosequencing in probably the simplest way possible.

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We are blessed to attend such amazing lectures and clarify our concepts 😃👍

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When you said "if there is no thymine, then adenine will not attach and light will not be produced" I almost fell in love with you, you've saved my day. I am forever grateful. Keep doing your amazing work and congrats for the studying you have done in order to reach this level of understanding.

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[@syedasifullah9797]

THANKYOU so much youhave helped me to understand prosequencing very well

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[@aritrajit9279]

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[@OniSing]

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explanation is awesome

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Thankyou so much sir 🙏😊 great explanation

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[@afreenshaikh5016]

This is very nice. Great! I wanted to request for targeted next gene sequencing. please make videos on this for better understanding, your explanation is too good. Thank you so much for making us to understand the pyrosequencing.

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[@Everything-uk7ce]

I really like it bro. make a video about shotgun and clone contig sequencing

[@sayansarkar8546]

Shomu da very informative video. There is one slight mistake : APS is adenosine 5' phosphosulphate. I have one doubt what is the use of adenosine alpha thiophosphate ? Btw great video Ur follower from KOLKATA ♥️♥️

[@rezoanulfahim8237]

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You're welcome

[@SunilSingh-cn1iy]

APS is adenosine 5' phosphosulfate, not ammonium persulphate.

[@hedayathabibzai6570]

@@sinthy8447. does pointing out the mistake of someone is bad????

[@hedayathabibzai6570]

thank u brother

[@shubhagatasarkar]

@@sinthy8447 What is wrong in pointing out mistakes. What is even wrong in making mistakes! It is just human error.

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@@shubhagatasarkar exactly!

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this was helpfull Sir

[@kavitarawat9544]

please make video on genome finishing - gaps and their resolution would be very helpful! ❤

[@shomusbiologyofficial]

You're welcome

[@priyamahajan9980]

Very well explained. I have query : In a well we have multiple fragments (having varied sequence), then how can we detect the sequencing because at the same time nucleotides are added at the multiple fragments ?

[@ankitachowdhury5268]

Really love you Dada...

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[@mehulpatel1572]

thank you brother.....

[@MuhammadIslamag]

You are doing great job bro. APS is Adenosine phosphosulfurase

[@shomusbiologyofficial]

Yes it is

[@muhammadzaheer8550]

Welldone sir....

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[@mdfaizurrahman5844]

very convoluted lecture

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U are the best 👍🏻👍🏻

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[@dr.tushar3527]

Good explaination

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Thank you

[@kouapatrick3838]

Well explained my dear !

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+KOUA Patrick thank you very much

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Lovely!

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Thank u soo much sir. It means a lot...

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much useful .. thankyou

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[@ashwindaund7818]

Sir this type of video gives more understanding rather than animated one...

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Thank you. Glad you liked my lectures

[@zubairtabishnaats2343]

At one time we add many nucleotides of same type or one only in the wells?

[@sayalikurundkar1490]

Thank you soo much sir for helping us out to clear our doubts.Sir in the video you have by mistakenly told the fullform of APS as Amonium persulfate but it should be( Adenosine 5' -phosphate sulfate )

[@shomusbiologyofficial]

Yes sorry

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Thank you!❤

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Thank you dada.....

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[@shivaniadityasinha3591]

Thks sir

[@chandrat1221]

Thanks! what is the sequencing QiaSeq target amplicon method different from Sanger sequencing method ? which is superior?

[@shilpasubash8844]

thank u sir.

[@shomusbiologyofficial]

+Shilpa Subash you're welcome

[@fenix1659]

Hello, it's a very interesting video, I have some questions, How are the percentages of methylation obtained by the equipment generated? that is, how are they generated? How do you get that percentage? I have looked for this information but I have only found the basis of the chemical reaction but not of obtaining the percentage, I hope you can help me solve my questions, thank you very much

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Thanks, man 👍.

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Nice information

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best and the easiest I've seen so far

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Thank you

[@prajakta7817]

Very well explained ! Only one thing .... APS is Adenosine 5' phosphosulfate !

[@r.yaminiragu8832]

awesome

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Kindly suggest some good book to study ngs in detail

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Perfect👍👍

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Super boss