Quantitative Western Blotting: Optimization is the Key

This video will be discussing the quantitative western blotting workflow and how optimization is critical for achieving reproducible data. Presented by Dr. Sean Taylor.
Covered topics include:
0:00 - Overview of western blotting workflow
1:27 - What optimize?
2:40 - Optimization of sample preparation & electrophoresis running condition
7:14 - Optimization of protein load
14:42 - Optimization of blocking and antibody incubation
18:39 - Optimization of detection and quantitation
25:06 - Stain-free gel technology
26:31 - References
See products at www.bio-rad.com/westernblotting
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