RAST: Bacterial genome sequence annotations.

[@ehecat]

man, thank you so much, my mind just blew out... I thought I knew how to use RAST... big big B.I.G MISTAKE... really really thank you!!

[@genomeprojects]

Great, I only really use RAST for annotations. RAST does have a genome browser and comparison tool also but I don't use these so much.

[@kasthurisivakumar9034]

Gettings sir I am a Microbiology scholar from India. I want to check the presence of a particular gene in a bacterial genome. How can I search a specific gene sir. Please solve my problem sir.

[@genomeprojects]

If you have annotated your genome using RAST, there are options to search using the SEED viewer. The viewer allows search for key words, or search by function etc. Finding a gene by name may be dependant on whether it has been suitably annotated rather than noted as "hypothetical" by RAST. If the SEED viewer is no help, you could BLAST search your entire genome with your gene (or similar) sequence, the BLAST search will then allow you to identify the gene coordinates within your annotation file, there you should find the gene.

[@kasthurisivakumar9034]

@@genomeprojects ok sir. I have another doubt sir. How to identify the gene location or locus tag/ number in rast sir.

[@bdt2y]

Thanks for the video, I want to ask how to register an account at RAST

[@genomeprojects]

Hi, I took this information directly from the RAST website. Go to RAST and within the login screen, select "register for a new account". You need to enter your first and last name as well as your email address into the required fields. Then select your country and choose a login name. After a RAST administrator has approved your account, RAST send you an email confirming account approval, and explaining how to login and set your password.

[@ashishbhatt3467]

how did you create that thumbnail image on RAST??

[@genomeprojects]

Hi, it was created using screen clips from the website. These were merged in PowerPoint then saved and uploaded as a Jpeg.

[@HolyAnchor]

Misleading thumbnail

[@genomeprojects]

Hi, my apologies if you find it misleading. Thumbnail is from a screenshot taken of the RAST website, as per the contents of the video.