Reconstituting a dikaryon from a streak plate or G & D, pulling monokaryons, making a squash mount.

[@josephfuller2716]

Very helpful thank you 😢😊

[@Hyphahypeman]

Great video, exactly what the people need, easy to understand and straight to the point

[@edwardgrand]

Thank you for watching. I have some straight to the point. Others, not so much. You should check out my 4 hour lives ;) LOL

[@Hyphahypeman]

@ lol yeah man I have adhd, sometimes I can keep it straight to the point and not get distracted at all or I can end up spending ten times longer than I should explaining completely unnecessary things and going into random rabbet trails. I think you do a good job though I enjoy you’re videos

[@hotamale35]

Well sir, another gr8 video. Always informative and the knowledge and practices I've acquired about mycology from you since running across your videos is astounding.... actually like your longer videos better than your short ones. I wish my high school biology teacher did itlike you back in the day... keep up the great work sir ty... if you ever visit nor-cal or I hit up 🇹🇭.. drinks are on me....

[@edwardgrand]

Thank you. I'm glad to help. I used to be a teacher, but my subject was kind of boring to teach. I'm having much more fun now.

[@starburstmemories]

Man.. thank you, this vid is right on point for me right now. You clarified 3 topics in one go! 🙏 I’ve started pulling pieces off scarce g&d plates like you but wasn’t sure about myself. Great to see it’s a thing! Though I would’ve never dared chop’em up together like that till I saw you do it. Awesome. Secondly, the size of squash plate pieces and how hard you push… super helpful. And last, I have a ton of RW plates cause.. I find it very temperamental and shy on agar. Is it just me or generally true?

[@BigMush9000]

Great video, Ed!

[@bebophippie1781]

Thank you once again.

[@SPORE_SPORTS]

Hello ED1K!

[@Primitivemycology]

Great explanation Ed! U mentioned a cosmetics sterilizer, is it the glass bead type?

[@edwardgrand]

yes. Those are the words I was looking for :)

[@Primitivemycology]

@edwardgrand that's awesome! Do you like it? I have an induction sterilizer but as you know it requires a pause to sterilize each time. I have wondered how effective the bead sterilizers are because I would love to be able to put 4 scalpel and some tweezers in there at a time and grab as I need while they stay sterile. Seems it would be more effective than what I'm doing now

[@edwardgrand]

@@Primitivemycology That is exactly what I do. They get a little hot, so I take them out and rest the on my alcohol jar about 30 seconds before I use them. $10 online.

[@Primitivemycology]

@edwardgrand wow that's cheap. I'm sold, even if they are a little more in America. I will have to check it out

[@yanmo2500]

Coool

[@bebophippie1781]

Hey Ed. I have a lot of mushroom compost that I want to use in my garden next year. How would you store it? I was thinking about mixing it 50/50 with some soil in lidded 5-gallon buckets. I get heavy winters where I'm at so I wasn't sure about just piling them up outside. I do have multiple compost piles already but I thought the mushroom compost might be worth keeping separate for some reason. Perhaps even for resale.

[@edwardgrand]

I would just throw it in the compost pile. Keeping it indoors sounds like a magnet for contamination and bugs.

[@bebophippie1781]

@@edwardgrand Yeah I was kind of leaning that way as well. I think I will just start a separate compost for it. Perhaps toss some straw on it every now and then and see what comes out of it. Thanks.

[@ElTrupii]

Hey do you reuse your agar plates or are they disposable

[@edwardgrand]

They are disposable Polystyrene (PS).

[@BigfootPrinting]

Doo doo doo, hello dr ed! No need to guess which transfer you took, you have the video to recall! Also a question: when I’m streaking with swabs, I feel like I’m just getting agar accumulated on the swab rather than depositing spores onto the agar. When the swab surface gets covered in the agar, it becomes like a slick coating over where the spores are sitting in the fingers. Do you think it would be better to rip off or fray the swab tip and swab with that? I’m not trying to get monos, just usable di’s. My streak plates won’t germinate, and I need some advice

[@edwardgrand]

I realized that after I uploaded the video too :) I figured it out and relabeled them. Same here with the streaking. I usually get enough germination points, but sometimes I will take the scalpel and rip the whole gunky end off and embed it in the gel. You might want to try the gellan gum. I am getting way better results with it for germ plates.

[@BigfootPrinting]

@ do you have a resource you could point to for how to prepare the Gellan gum media? I’d really appreciate it!

[@edwardgrand]

@@BigfootPrinting sporeswaps.com/shop/mycology-supplies/gellan-gum-250gram-pack-low-acyl-gelling-agent-for-ultra-clear-microscopy-plates/ >> Gellan Gum is an excellent alternative to traditional agar-based recipes. It tends to be clearer, more rigid, and has a higher melting temperature (great for shipping in warm climates). GENERAL RECIPE 0.8% Gellan Gum (low acyl) 1.2% LME (light malt extract) 0.1% Nutritional Yeast 0.1% MgSO4*7H20 (Epsom Salt) 0.01% Dye FOR 1X RECIPE (500 mL) 4g Gellan Gum (low acyl) 6g LME (light malt extract) 0.5g Nutritional Yeast 0.5g MgSO4*7H20 (Epsom Salt) 0.05g Dye

[@MycoAlan]

👍🏻