restriction fragment length polymorphism (RFLP) |Principle | applications | limitations

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Great Explanation!! ..... Please use the common font styles in writing, like Arial or else!

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[@ARYA__2000]

It was easy and helpfull

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[@aarjvijain]

Alec Jeffrey gave DNA fingerprinting, not RFLP... are they the same thing? I don't think so

[@saifalhasan9895]

Why Southern blotting is used in RFLP? What if I didn't do the southern blotting part?

[@animatedbiologywitharpan]

in RFLP the GOAL is to detect specific DNA fragments after restriction enzyme digestion and gel electrophoresis. Now imagine the fragments are not transfered in the membrabe. Then hybridization is not possible. In gel hybridization cant be done.

[@WoogaChan]

My teacher used this for our cancelled lesson

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Thanks

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[@chriswong1439]

Thank you for your video!! But I have a question. Can I say that Southern Blotting is a technique for detecting RFLP? or RFLP itself is a different technique?

[@animatedbiologywitharpan]

In short Rflp use southern blot method. Watch the video : kzbin.info/www/bejne/pGPFh3mZf7Vgfrs

[@chriswong1439]

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[@tehreemsajjad653]

Rflp used in SNP analysis??

[@animatedbiologywitharpan]

If SNP alters a restriction sequence can be genotyped by 'natural PCR' after restriction digestion.

[@tehreemsajjad653]

So it's mean we use SNP analysis for RFLP?

[@tehreemsajjad653]

Did you know SNP analysis techniques??

[@tehreemsajjad653]

Sir reply plz

[@tamilbiology5078]

Can we detect point mutations with this technique?

[@animatedbiologywitharpan]

Not reliably ....point mutation might change a restriction cleavage site hence the restriction fragments in theory should be of different length in control and mutant. But now a days pcr based methods / sequencing methods are used

[@walaasaeed6960]

Thanks 👍🏻

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[@walaasaeed6960]

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[@gulpypup1069]

may I know what international issues of RFLP if there is one?

[@animatedbiologywitharpan]

RFLP is a labor-intensive procedure that does not have the resolving power anywhere closer that of WGS and SNP analysis.

[@reshu6012]

Sir it is possible to produce large fragments of DNA using PCR no sir From the available crime scene samples?

[@animatedbiologywitharpan]

Pcr can amplify upto 500bp dna reliably beyond that efficiency drops badly. So in short yes it could be done but for short dna fragments but not the entire genome.

[@rashmisekar3852]

Can linkage mapping be done using this method? If so, how do we find out if a person is homozygous or heterozygous for a particular gene?

[@animatedbiologywitharpan]

Noting can be done very accurately with this method. Its obsolete because of highthroughput sequencing. Most RFLP markers are co-dominant (both alleles in heterozygous sample will be detected) and highly locus-specific.

[@pjshady7465]

3 minutes but video is 6 minutes 😂

[@animatedbiologywitharpan]

I hope it is informative enough